Detection of mutant infectious bronchitis viruses of GI-23 lineage from commercial chicken flocks in Khorasan Razavi province, Iran in 2019

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 12 اسفند 1399  

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2021.68272.1009

نویسندگان

Seyed-Elias Tabatabaeizadeh ¹؛ Reza Toroghi¹؛ Naser Margan Azghadi²؛ Hamidreza Farzin¹؛ Shahram Sharghi²؛ Mojgan Sarani¹؛ Majid Jamshidian Mojaver¹؛ Javad Alami Abardeh²؛ Mahmoud Ghorbanzadeh²؛ Maryam Torabi²؛ Alireza Sadrebazzaz¹؛ Mojtaba Fakhraei¹؛ Nafiseh Keyvanirad²؛ Elnaz Vojudi¹؛ Majid Khodaverdi Azghandi²؛ Tabasom Mardani²

¹Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

²Veterinary Head Office of Khorasan Razavi, Mashhad, Iran

چکیده

Infectious bronchitis (IB), caused by infectious bronchitis virus (IBV), is one of the most important respiratory diseases in poultry. The implementation of preventive measures, including vaccination and biosecurity, is necessary for controlling the disease. To maintain biosecurity, it is important to identify the entry route of new viruses into a region, and characterizing markers such as unique mutations make viruses traceable. During a genotyping study for IBV infected commercial chicken flocks in Khorasan Razavi province, 11 viruses from 11 broiler and layer chicken flocks were detected in different cities by molecular method PCR. Sequencing of the S1 gene variable region followed by phylogenetic analysis showed that eight viruses are classified in GI-23 lineage (Is-Variant2), two viruses are classified in GI-1 lineage (Mass), and one virus is classified in GI-12 lineage (793B). Although detected viruses of GI-23 lineage are originated from Iran, seven viruses have synonymous (T954C and G1056A) and non-synonymous (C797T) mutations that have not been previously reported. It was found that the new genetic changes in Iranian IBVs of GI-23 lineage occurred in two different regions in Khorasan Razavi. In conclusion, this study indicates that the high prevalence of GI-23 lineage viruses in Iran may enhance the chance of virus mutations and the emergence of new viral strains, so effective vaccination and biosecurity measures are required to control the virus spread.

کلیدواژه‌ها

IBV؛ Genotyping؛ GI-23 lineage؛ Mutation؛ Iran