LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer

Journal of Cell and Molecular Research

مقاله 1، دوره 12، شماره 2 - شماره پیاپی 24، خرداد 2021، صفحه 49-55   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2021.67052.0

نویسندگان

Seyedeh Nahid Fotuhi¹؛ Mohammad Khalaj-Kondori^{* 1}؛ Hadis Karimi²

¹Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

²Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده

     Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis. However, the expression level of this lncRNA in ovarian cancer is not determined.50paired ovarian tumors and their adjusted normal tissues were included in the study. Total RNA was extracted by TRIzol^® Reagent and reverse-transcribed to cDNA using PrimeScript II cDNA synthesis kit. The expression levels of HOXD-AS1 were quantified by qRT-PCR and compared. The Roc curve analysis was used to evaluate the capacity of HOXD-AS1 as a biomarker for ovarian cancer. We observed that lncRNA HOXD-AS1 was significantly upregulated in ovarian tumors compared to their adjusted normal tissues (p <0.003). Moreover, the ROC curve analysis revealed that the lncRNA HOXD-AS1 expression level could discriminate tumoral and non-tumoral tissues with 85% sensitivity and 88% specificity. The lncRNA HOXD-AS1 expression level might be considered as a potential biomarker for ovarian cancer development.  

کلیدواژه‌ها

Ovarian cancer؛ Biomarker؛ lncRNA؛ HOXD-AS1؛ Gene expression

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