اثر تنش خشکی بر متغیرهای جوانه‌زنی بذر و صفات فیزیولوژیکی گیاه ختمی زینتی (Alcea rosea L.)

علوم باغبانی

مقاله 3، دوره 35، شماره 4 - شماره پیاپی 52، اسفند 1400، صفحه 479-492   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jhs.2021.60838.0

نویسندگان

تکتم اورعی؛ محمود شور^* ؛ علی تهرانی فر؛ سید حسین نعمتی

گروه علوم باغبانی و فضای سبز، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

گل ختمی (Alcea rosea L.) یکی از گیاهان با ارزش زینتی می‌باشد که 36 گونه از آن در ایران کشت و کار می‌شود. با توجه به این مسئله که جوانه‌زنی بذور در طبیعت با تنش خشکی همراه است لذا میتوان با مطالعه­ی تغییرات فیزولوژیکی و آنتی اکسیدانی گامی مهم در راستای احیای رویشگاه‌های آن برداشت. بدین منظور آزمایشی در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه دانشکده کشاورزی دانشگاه فروسی مشهد اجرا شد. تیمارهای آزمایش شامل دو اکوتیپ مشهد و تهران با پنج سطح خشکی (صفر، 2-، 4-، 6- و 8- بار) بود. نتایج نشان داد که اثر اکوتیپ و سطوح خشکی بر تمام صفات اندازه‌گیری شده معنی‌دار بود. اکوتیپ مشهد دارای برتری معنی‌داری نسبت به اکوتیپ تهران بود و بذر آنها توانست در تیمارهای 6- و 8- بار به ترتیب 28 و 24 درصد جوانه بزند. میزان طول ریشه‌چه و ساقه‌چه و وزن اکوتیپ تهران با افزایش پتانسیل اسمزی نسبت به اکوتیپ مشهد بیشتر کاهش یافت. از طرفی فعالیت آنزیم‌های مورد بررسی نشان داد که در هر دو اکوتیپ فعالیت سوپراکسید دیسموتاز و پراکسیداز کاهش یافت اما فعالیت آنزیم کاتالاز با کاهش بیشتر پتانسیل اسمزی افزایش یافت. میزان پرولین و مالو‌ن‌دی‌آلدهید در اکوتیپ مشهد در تیمار 8- بار نسبت به شاهد به‌ترتیب 12 و 1/5 برابر شد. به نظر می‌رسد که اکوتیپ مشهد دارای بیشترین تحمل به خشکی را در مقایسه با اکوتیپ تهران در مرحله جوانه‌زنی دارد. از طرفی همبستگی مثبت و معنی‌داری بین فعالیت آنزیم سوپراکسید دیسموتاز و پراکسیداز با درصد جوانه‌زنی مشاهده شد.

کلیدواژه‌ها

پتانسیل اسمزی؛ ختمی زینتی؛ درصد جوانه‌زنی؛ شاخص‌های فیزیولوژیکی و بیوشیمیایی

مراجع

1. Abdollahi J., Tafaroji S.H., Ebrahimi M., Ramshini H.A., Dehghani Tafti A.R., Mortazavian M., Eftekhari M., Dezfoulian A.H., and Bagheri A. 2012. Effect of drought stress on germination and seedling growth of Salvia African Journal of Agricultural Research 7: 5719-5725.
2. Abido W.A.E., and Zsombik L. 2018. Effect of water stress on germination of some Hungarian wheat landraces varieties. Acta Ecologica Sinica 38: 422-428.
3. Achakzai A.K.K. 2014. Imbibition, germination and lipid mobilization response by sunflower subjected to salt stress. Pakistan Journal of Botany 46: 1995-2002.
4. Agrawal R.L. 1991. Seed Technology. Oxford & IBH Publishing, 658p.
5. Ahmed B., Roy C.D., Subramaniam S.A., Ganie D., Kwatra D., Dixon D., Anant A., Zarqar M.A., and Umar S. 2016. An ornamental plant targets epigenetic signaling to block cancer stem cell driven colon carcinogenesis. Carcinogenesis 37: 385-396.
6. Akhavan Barmaki M., Azanivand H., Osare M.H., Jafari Y.A., and Tavili A. 2013. Evaluation of drought stress effects on germination indices of four genotypes grassland of Bromus tomentellus. Agricultural Water Management Journal 66: 167-177. (In Persian with English abstract)
7. Akhavan Barmaki M., Hashemi M., Azarnivand H., Osare M.H., Jafari A.A., and Tavili A. 2013. Effect of drought stress on growth and germination characteristics of three genotype of Agropyron trichophorum. Desert Ecosystem Engineering Journal 2: 9-14. (In Persian with English abstract)
8. Alscher R., Heath A., Erturk N., and Heath L.S. 2002 Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany 53: 1331-1341.
9. Bagheri H., Ghazi Khanloosani Y., Zangani E., Andelibi B._, Azimi Moghadam M.R., and Jamshidi S. 2012. Seed germination indices and initial growth of safflower seedlings with different thousand kernel weights under drought stress. Agroecology Journal 8: 1-12. (In Persian with English abstract)
10. Bates L.S., Waldren R.P., and Teare I.D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205-207.
11. Burt S.A., and Reinders R.D. 2003. Antibacterial activity of selected plant essential oils against Escherichia coli O157: H7. Letters in Applied Microbiology 36: 162-167.
12. Chen K., and Arora R. 2011. Dynamics of the antioxidant system during seed osmopriming, post-priming germination, and seedling establishment in Spinach (Spinacia oleracea). Plant Science 180: 212-20.
13. Chen Z., Wang Z., Yang Y., Li M., and Xu B. 2018. Abscisic acid and brassinolide combined application synergistically enhances drought tolerance and photosynthesis of tall fescue under water stress. Scientica Horticulturae 228: 1-9.
14. Ebadi M.T., Azizi M., and Farzane A. 2011. Effect of drought stress on germination factors of four improved cultivars of german chamomile (Matricaria recutita ). Journal of Plant Product 18: 119-132. (In Persian with English abstract)
15. Fabian A., Jager K., and Barnabas B. 2008. Effects of drought and combined drought and heat stress on germination ability and seminal root growth of wheat (Triticum aestivum) seedlings. Journal of Acta Biologica 52: 157-159.
16. Fathi A., and Barari Tari D. 2016. Effect of drought stress and its mechanism in plants. International Journal of Life Sciences 10: 1-6.
17. Gao J.M., Xiao Q., Ding L.P., Chen M.J., Yin L., Li J.Z., Zhou S., and He G. 2008. Differential responses of lipid peroxidation and antioxidants in Alternanthera philoxeroides and Oryza sativa subjected to drought stress. Plant Growth Regulation 56: 89-95.
18. Ghodke P.H., Andhale P.S., Gijare U.M., Thangasamy A., Khade Y.P., Mahajan V., and Singh M. 2018. Physiological and biochemical responses in onion crop to drought stress. International Journal of Current Microbiology and Applied Sciences 7: 2054-2062.
19. Giannopolitis C.N., and Ries S.K. 1977. Superoxide dismutases. I. Occurrence in higher plants. Plant. Physiology 59: 309-314.
20. Gill S.S., and Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48:909-930.
21. Guo Q., Wang Y., Zhang H., Qu G., Wang T., Sun Q. and Liang D. 2017. Alleviation of adverse effects of drought stress on wheat seed germination using atmospheric dielectric barrier discharge plasma treatment. Scientific Reports 7: 1666-1680.
22. Hasanuzzaman M., Anwar Hossain M., Teixeira daSilva J.A., and Fujita M. Plant Response and Tolerance to Abiotic Oxidative Stress: Antioxidant Defense Is a Key Factor. Crop Stress and its Management: Perspectives and Strategies, pp 261-315.
23. Heath R.L., and Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125: 189-198.
24. Hussain L., Akash M.S.H., Tahir M., Rehman K., and Ahmed K.Z. 2014 Hepatoprotective effects of methanolic extract of Alcea rosea against acetaminophen-induced hepatotoxicity in mice. Bangladesh Journal of Pharmacology 9: 322-327.
25. In, B.C., Motomura S., Inamoto K., Doi M., and Mori G. 2007. Multivariente analysis of relation between preharvest environmental factors, postharvest morphological and physiological factors and vase life of cut Asomi Red Roses. Jpn. Soc. Hortic. Sci. 76: 66-72.
26. 2014. In: R.K. Pachauri and L.A. Meyer (eds.), Climate Change 2014: Synthesis Report. Contribution of Working Groups I, II and III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. IPCC, Geneva, Switzerland, 151 pp.
27. Jajarmi V. 2009. Effect of water stress on germination indices in seven wheat cultivar. World Academy of Science Engineering and Technology 49: 105-106.
28. Khalid M.N., Iqbal H.F., Tahir A., and Ahmad A.N. 2001. Germination potential of chickpeas (Cicer arietinum) under saline condition. Journal of Biological Science 4: 395-396.
29. Kusvuran S., and Dasgan H.Y. 2017. Drought induced physiological and biochemical responses in Solanum lycopersicum genotypes differing to tolerance. Acta Scientiarum Polonorum Hortorum Cultus Horticulture 16: 19-27.
30. Li H., Li X., Zhang D., Liu H. and Guan K. 2013. Effects of drought stress on the seed germination and early seedling growth of the endemic desert plant Eremosparton songoricum (Fabaceae(. Excil Journal 12: 89-101.
31. Lim T.K. 2014. Edible medicinal and nonmedicinal plants. Flowers 8: 292-299.
32. Liu M., Li M., Liu K., and Sui N. 2015. Effects of drought stress on seed germination and seedling growth of different maize varieties. Journal of Agricultural of Science 7: 231-240.
33. Luck H. 1974. Catalase. In Methods of Enzymatic Analysis, Vol. II, edited by J Bergmeyer and M Grabi, Academic Press, New York. Pp 885-890.
34. Mantovani A., and Iglesias R.R. 2010. The effect of water stress on seed germination of three terrestrial bromeliads from restinga. Brazilian Journal of Botany 33: 201-205.
35. Michel B.E., and Kaufman M.R. 1973. The osmotic potential of polyethylene glycol 6000. Physiology 51: 914-916.
36. Noman A., Aqeel M., Deng J., Khalid N., Sanaullah T., and Shuilin H. 2017. Biotechnological advancements for improving floral attributes in ornamental plants. Frontiers in Plant Science8: 1-15.
37. Pakravan M., and Ghahreman A. 2003: Some new combinations and synonyms in Alcea (Malvaceae) from Iran. Annalen des Naturhistorischen Museums in Wien 104: 713-716.
38. Penfield S. 2017. Seed dormancy and germination. Current Biology 27: 874-878.
39. Şevik H., and Ertürk N. 2015. Effects of drought stress on germination in fourteen provenances of Pinus Brutia seeds in Turkey. Turkish Journal of Agriculture - Food Science and Technology 3: 294-299.
40. Seyyednejad S.M., Koochak H., Darabpour E., and Motamedi H. 2010. A survey on Hibiscus rosa sinensis, Alcea rosea and Malva neglecta Wallr as antibacterial agents. Asian Pacific Journal of Tropical Medicine 3: 351-355.
41. Seyyed-Sharifi R., and Seyyed-Sharifi R. 2009. The effects of polyethyleneglycol on germination and seedling growth of carthamus cultivars. Iranian Journal of Biology 21: 400-410. (In Persian with English abstract)
42. Stefania Toscano S., Romana D., Tribulata A., and Patane C. 2017. Effect of drought stress on seed germination of ornamental sunflowers. Acta Physiologiae Plantarum 39:1-12.
43. Sun Y.D., Du X.H., Zhang W.J., Sun L., and Li R. 2011. Seed germination and physiological characteristics of Amaranthus mangostanus under drought stress. Advanced Mater Research Switzerland 183: 1071–1074.
44. Tajamolian M., Sodaei zadeh H., and Shamsaie M. 2017. Evaluation of the effects of drought stress of mother plants on some seed germination indices of Satureja hortensis. Desert Management 8: 27-35. (In Persian with English abstract)
45. Wang W.B., Kim Y.H., Lee H.S., Kim K.Y., Deng X.P., and Kwak S.S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry 47: 570-577.
46. Yamada M., Morishita H., Urano K., Shiozaki N., Yamaguchi-Shinozaki K., Shinozaki K., and Yoshiba Y. 2005. Effects of free proline accumulation in petunias under drought stress. Journal of Experimental Botany 56: 1975-1981.
47. Yan D., Duermeyer L., Leoveanu C., and Nambara E. 2014. The functions of the endosperm during seed germination. Plant Cell Physiology 55: 1521-1533.
48. Zamani Z., Amiri H., and Ismaili A. 20018. Effect of drought stress on germination characteristics of two populations of Fenugreek (Trigonella foenumsubsp.graceum L.). Nova Biologica Reperta 5: 183-191. (In Persian with English abstract)