Effect of Tragacanth on some Morpho-physiological Characteristics related to Black Cumin (Nigella sativa L.) Yield under Drought Stress Conditions

علوم باغبانی

Article 14, Volume 35, Issue 4 - Serial Number 52, January 2022, Pages 631-645   

Document Type: Research Article

DOI: 10.22067/jhs.2021.69276.1033

Authors

Y. Sohrabi^* ; H. Kiani

Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

Abstract

Introduction: In fact, drought is stress that restricts the plant photosynthesis and also it causes of chlorophyll content changes and damage to photosynthetic structures. One of the important reasons that environmental stresses such as drought reduce the growth and photosynthesis ability of the plant is a disturbance in the balance between production and removal of free oxygen radicals. Transpiration is a necessary process for photosynthesis and growth of plants but depending on the conditions that may be harmful in some cases. Therefore, the use of anti-transpirant can be one of the most effective methods for reducing the amount of water lost through transpiration and adjustment the reduction of the yield due to water deficiency in arid and semi-arid regions. Climate change happened on earth and the intensification of stresses caused by it, especially drought stress in arid and semi-arid regions such as Iran. Therefore, finding strategies that can reduce the effects of water shortages on plant growth and yield can be very important. The aim of the present study was to investigate the effect of irrigation regimes and application of different concentrations of tragacanth (naturally dried exudate from some Astragalus species) on black cumin plant.   
Materials and Methods: This research was carried out in a factorial experiment based on completely randomized design with three replications in a greenhouse of the Agriculture College of Kurdistan University in 2018. The experimental factors were including irrigation at three levels of 100% (full irrigation), 70% (mild drought stress), and 40% (severe drought stress) of field capacity of soil and spraying with tragacanth extract at six concentrations of 0, 1.25, 2.5, 5, 7.5, and 10 g/L. Spraying of this material was done using a back sprayer (Shark model) with a constant pressure of 2.4 bar and a volume of 250 liters of water per hectare., The normality test was performed using the Mini Tab software, before the data were analyzed. After ensuring the normality of data, analysis of variance was performed using SAS ver. 9.3. LSD (Least significant difference) was used to compare the mean of treatments. The graphs are drawn using Excel software.   
Results and Discussion: The results showed that increased drought stress intensity (irrigation reduction) led to the reduced leaf relative water content, Total chlorophyll content, efficiency of photosystem II, plant height, number of capsules per plant, mean number of seeds per plant, biological yield and grain yield. The positive effects of tragacanth consumption on reducing and modifying the effects of drought stress on different levels of irrigation and different concentrations of tragacanth were different. In the present study, under full irrigation conditions, lower concentrations of tragacanth were useful, while in drought stress conditions, higher concentrations of tragacanth (except 10 g/L) were useful. In full irrigation, the concentration of 1.25 g/L was positive for all studied traits. In mild drought stress, the use of higher concentrations of tragacanth up to 5 g/L had the best effect and more concentrations resulted in a reverse effect on studied traits. In severe drought stress, the use of more concentrations of tragacanth extract was beneficial and improved the studied traits up to 7.5 g/L, but 10 g/L had a negative effect on these traits.
Conclusion: The results of this study indicated that the different effects of various concentrations of tragacanth material in different levels of irrigation on studied traits of black cumin. Therefore, it can be concluded that the application of different concentrations of tragacanth gum was completely dependent on the plant's water status. Therefore, using higher concentrations of tragacanth gum in drought stress conditions had a more positive effect on the plant, and vice versa, using a lower concentration of this material was useful in full irrigation. The effect of tragacanth gum on reducing and modifying the effects of drought stress in different plants requires further studies and extensive research. Tragacanth gum can be introduced as a new anti-transpirant agent with natural origin and its application can be useful and recommended in areas exposed to drought stress.
 

Keywords

Anti-transpirant agent; Chlorophyll; Grain yield; Photosystem II efficiency; Relative water content

References

1. AbdAllah A.M., Mashaheet A.M., Zobel R., and Burkey K.O. 2019, Physiological basis for controlling water consumption by two snap beans genotypes using different anti-transpirants, Agricultural Water Management 214: 17-27.
2. Abdullah A.S., Aziz M.M., Siddique K.H.M., and Flower K.C. 2015, Film antitranspirants increase yield in drought stressed wheat plants by maintaining high grain number, Agricultural Water Management 159: 11-18.
3. Arnon A.N. 1967. Method of extraction of chlorophyll in the plants, Agronomy Journal 23: 112-121.
4. Blum A. 2005. Drought resistance, water-use efficiency, and yield potential-are they compatible, dissonant, or mutually exclusive?, Australian Journal of Agricultural Research 56: 1159-1168.
5. Cheeseman 2016. Food Security in the Face of Salinity, Drought, Climate Change, and Population Growth. P. 111-123. In: Khan M.A., Ozturk M., Gul B., Ahmed M.Z. (eds.) Halophytes for Food Security in Dry Lands. Academic Press, San Diego.
6. Davenport D.C. 1967. Effects of chemical antitranspirants on transpiration and growth of grass, Journal of Experimental Botany 18: 332-347.
7. Davenport D.C., Hagan R.M., and Martin P.E. 1969. Antitranspirant uses and effects on plant life, California Agriculture 23(5): 14-16.
8. Del Amor F.M., and Jose Rubio S. 2009. Effects of Antitranspirant spray and potassium: calcium: magnesium ratio on photosynthesis, nutrient and water uptake, growth, and yield of sweet pepper, Journal of Plant Nutrition 32(1): 97-111.
9. Fallah, Malekzadeh S., and Pessarakli M. 2017. Seed priming improves seedling emergence and reduces oxidative stress in Nigella sativa under soil moisture stress, Journalof Plant Nutrition 41(1): 29-40.
10. Faralli M., Grove I.G., Hare M.C., Alcalde‐Barrios A., Williams K.S., Corke F.M.K., and Kettlewell P.S. 2017. Modulation of Brassica napus source-sink physiology through film antitranspirant induced drought tolerance amelioration that is dependent on the stress magnitude, Journal of Agronomyand Crop Science 203(5): 360-372.
11. Farooq M., Wahid A,. Fujita D., Basra S.M.A., and Kobayashi N. 2009, Plant drought stress: Effects, Mechanisms and Management, Agronomy for Sustainable Development 29: 185-212.
12. Flexas J., NIInemets Ü., Gallé A., Barbour M.M., Centritto M., Diaz-Espejo A., Douthe, C., Galmés J., Ribas-Carbo M., and Rodriguez P.L. 2019. Diffusional conductances to CO₂ as a target for increasing photosynthesis and photosynthetic water-use efficiency, Photosynthesis Research 65(8): 18-23.
13. Haj Seyed Hadi M.R., Darzi M.T., and Riazi G.H. 2016. Black cumin (Nigella sativa) yield affected by irrigation and plant growth promoting bacteria, Journal of Medicinal Plants and By-products 2: 125-133.
14. He J., Du Y.L., Wang T., Turner N.C., Yang R.P., Jin Y., Xi Y., Zhang C., Cui T., Fang X.W., and Li F.M. 2017. Conserved water use improves the yield performance of soybean (Glycine max (L.) Merr.) under drought, Agricultural Water Management 179: 236-245.
15. IrigoyenJ., Goicoechea N., Antolín M.C., Pascual I., Sánchez-Díaz M., Aguirreolea J., and Morales F. 2014. Growth, photosynthetic acclimation and yield quality in legumes under climate change simulations: An updated survey, Plant Science 226: 22-29.
16. Irmak A., Jones J.W., Stanley C.D., Hansen J.W., Irmak S., and Boote K.J. 1999. Some effects of an antitranspirant (Vapor Gard) on tomato growth and yield, Soil and Crop Science 58: 118-122.
17. Jaleel A., Manivannan P., Vahid A., Farooq M., Al-Juburi H.J., Somasudram R., and Vam R.P. 2009. Drought stress in plants: A review on morphological characteristics and pigments composition, Journalof Agriculture and Biology 11(1): 100-105.
18. Jeon M.W., Ali M.B., Hahn E.J., and Paek K.Y. 2006. Photosynthetic pigments, morphology and leaf gas exchange during ex-vitro acclimatization of micropropagated CAM Doritaenopsis plantlets under relative humidity and air temperature, Environmentaland Experimental Botany 55: 183-194.
19. João V.A.C., Joaquim A.G.S., Fabrício E.L.C., Juliana R.C., and Milton C.L.N. 2019. The regulation of P700 is an important photoprotective mechanism to NaCl-salinity in Jatropha curcas, Physiologia Plantarum 167(3): 404-417.
20. Kabiri R., Nasibi F., and Farahbakhsh H. 2014. Effect of exogenous salicylic acid on some physiological parameters and alleviation of drought stress in Nigella sativa plant under hydroponic culture, Plant Protection Science 50: 43-51.
21. Kettlewell P.S., Heath W.L., and Haigh I.M. 2010. Yield enhancement of droughted wheat by film antitranspirant application: rationale and evidence, Journal of Agricultural Science 1: 143-147.
22. Lawlor D.W., and Cornic G. 2002, Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants, Plant and Cell Environmental 25: 275-294.
23. Li G.M., Liu B.B., Wu Y., and Zou Z.R. 2008. Interactive effects of drought stresses and elevated CO₂ concentration on photochemistry efficiency of cucumber seedlings, Journal of Integrative Plant Biology 50(10): 1307-1317.
24. Manavalan L.P., and Nguyen H.T. 2017. Drought Tolerance in Crops: Physiology to Genomics. p. 1-23. In: Shabala S. (eds) Plant Stress Physiology. Wallingford Oxfordshire, Boston.
25. Mascher R., Nagy E., Lippmann B., Hornlein S., Fischer S., Scheiding W., Neagoe A., and Bergmann H. 2005. Improvement of tolerance to paraquat and drought in barley (Hordeum vulgare) by exogenous 2-aminoethanol: effects on superoxide dismutase activity and chloroplast ultrastructure, Plant Science 168: 691-698.
26. Miyata K., Ikeda H., and Nakaji M. 2016. Rate constants of PSII photoinhibition and its repair, and PSII fluorescence parameters in field plants in relation to their growth light environments, Plant Cell Physiology 56: 1841-1854.
27. Moftah A.E. 1997. The response of soybean plants, grown under different water regimes, to antitranspirant applications, Annals of AgriculturalSciences 35: 263-292.
28. Pessarkli M. 1999. Handbook of plant and crop stress. Marcel Dekker, New York.
29. Prakash M., and Ramachandran K. 2000. Effects of chemical ameliorants on stomatal frequency and water relations in brinjal (Solanum melongena) under moisture stress conditions, Journal Agronomy Crop Science 185: 237-239.
30. Poormansour S., Razzaghi F., Sepaskhah A., and Moosavi A. 2019. Wheat growth and yield investigation under different levels of biochar and deficit irrigation under greenhouse conditions, Journal of Water and Irrigation Management 9(1): 15-27. (In Persian with English abstract)
31. Rezaei-Chiyaneh E., Seyyedi S., Ebrahimian M., Siavash E., Moghaddam S., and Damalas C.A. 2018. Exogenous application of gamma-aminobutyric acid (GABA) alleviates the effect of water deficit stress in black cumin (Nigella sativa) Esmaeil, Industrial Cropsand Products 112: 741-748.
32. Shao H.B., Chu L.Y., Jaleel C.A., and Zhao C.X. 2008. Water-deficit stress-induced anatomical changes in higher plants, Comptes Rendus Biologies 331: 215-225.
33. Shimakawa G., and Miyake C. 2018. Oxidation of P700 Ensures Robust Photosynthesis, Frontiers in Plant Science 172(3): 1443-1450.
34. Sinclair T.R., and LudlowM. 1985. Who taught plants thermodynamics? The unful filled potential of plant water potential, Australian Journal of Plant Physiology 12: 213-217.
35. Singh S., and Singh A. 1999. Use of dust mulch and anti-transpirant for improving water use efficiency of menthol mint (Mentha arvensis), Journal of Medicinal and Aromatic Plant Science 21: 29-33.
36. Sita K., Sehgal A., Kumar J., Kumar S., Singh S., Siddique K.H.M., and Nayyar H. 2017. Identification of high-temperature tolerant lentil (Lens culinaris) genotypes through leaf and pollen traits, Frontiers in Plant Science 8: 731-744.
37. Takagi D., Ishizaki K., Hanawa H., Mabuchi T., Shimakawa G., Yamamoto H., and Miyake C. 2017. Diversity of strategies for escaping reactive oxygen species production within photosystem I among land plants: P700 oxidation system is prerequisite for alleviating photoinhibition in photosystem I, Physiology Plant 161: 56-74.
38. Thakuria R.K., Singh H., and Singh T. 2004. Effect ofirrigation and antitranspirant on biometric components, seed yield and plant water-use of spring sunflower (Helianthus annuus), Indian Journal of Agronomy 49: 121-123.
39. Tikkanen M., Mekala N.R., and Aro E.M. 2014. Photosystem II photoinhibition-repair cycle protects photosystem I irreversible damage, Biochimicaet Biophysica Acta 1837: 210-215.
40. Tiryaki I. 2016. Drought stress and tolerance mechanisms in alfalfa (Medicago sativa), KSU Journal Natural Science 19: 296-305.
41. Xu Z., and Zhou Z. 2005. Effects of water stress on photosynthesis and nitrogen metabolism in vegetative and reproductive shoots of Leymus chinensis, Photosynthetica 43(1): 29-35.
42. Yadav R.S., and Bhushan C. 2001, Effect of moisture stress on growth and yield in rice genotype, Indian Journal Agriculture Research 2: 104-107.
43. Yang W., Guo S., Li P., Song R., and Yu J. 2018. Foliar antitranspirant and soil superabsorbent hydrogel affect photosynthetic gas exchange and water use efficiency of maize grown under low rainfall conditions, Journal of the Science of Food and Agriculture 99(1): 350-359.
44. YingQ., Song L.L., JacobsD.F., Mei L., Liu P., Jin S.H., and WU J.S. 2015. Physiological response to drought stress in Camptotheca acuminata seedlings from two provenances, Frontiers in Plant Science 6: 1-6.