بررسی ویژگی‌های مورفولوژیکی، عملکردی و فیتوشیمیایی گیاه دارویی پریلا ارغوانی (Perilla frutescens var. crispa) در سه مکان مختلف کشت

بوم شناسی کشاورزی

مقاله 10، دوره 14، شماره 3 - شماره پیاپی 53، مهر 1401، صفحه 549-560   

نوع مقاله: علمی - پژوهشی

شناسه دیجیتال (DOI): 10.22067/agry.2021.20226.0

نویسندگان

معصومه عیدوزایی¹؛ ابوذر علیزاده²؛ صمد نژاد ابراهیمی³؛ امیر صحرارو⁴؛ محمد حسین میرجلیلی³؛ جواد هادیان^{* 3}

¹گروه کشاورزی، پژوهشکده گیاهان و مواد اولیه دارویی دانشگاه شهید بهشتی، تهران، ایران.

²گروه کشاورزی، دانشگاه لرستان، خرم آباد، ایران و پژوهشگر جهاد دانشگاهی واحد استان لرستان، ایران.

³پژوهشکده گیاهان و مواد اولیه دارویی دانشگاه شهید بهشتی، تهران، ایران.

⁴گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران.

چکیده

به‌منظور ارزیابی سازگاری، رشد و برخی خصوصیات کمّی و کیفی گیاه دارویی پریلا ارغوانی (Perilla frutescens var. crispa) در ایران، این گیاه در سه مکان مختلف شامل ساری، رشت و شمال تهران کشت گردید. کشت گیاه دارویی پریلا ارغوانی در قالب طرح کامل تصادفی با سه تکرار در سه منطقه ساری، رشت و شمال تهران در اردیبهشت 1397 انجام شد. گیاهان در شروع گل‌دهی در مهر ماه برداشت شدند. ویژگی‌های مورفولوژیکی و عملکردی مورد مطالعه شامل ارتفاع بوته، طول گل‌آذین، تعداد ساقه، فاصله میان‌گره، قطر ساقه، عرض برگ، طول برگ، وزن تر و خشک برگ، وزن تر و خشک ساقه، وزن تر و خشک گل بودند. صفات فیتوشیمایی مورد مطالعه شامل محتوای فنل و فلاونوئید کل و میزان رزمارینک اسید بودند. نتایج تجریه واریانس نشان داد که اثر مکان‌های مختلف کشت بر همه صفات مورفولوژیکی (به‌جز وزن تر ساقه، وزن تر و خشک بوته) و صفات فیتوشیمیایی در سطح پنج درصد معنی‌دار بود. عملکرد خشک اندام دارویی (برگ و گل) در مکان­های کشت ساری (63/41و 05/32)، رشت (13/26 و71/12) و شمال تهران (99/25 و 47/ 18) گرم در مترمربع به‌دست آمد. محتوای فنل کل و رزمارینیک اسید گیاهان در رشت (به‌ترتیب مقدار027/ 49 و 29/42 میلی‌گرم بر گرم ماده خشک) بیشتر از سایر مناطق کشت بود، در حالی که فلاونوئید کل در منطقه ساری بیشترین مقدار (8/14 میلی‌گرم بر گرم ماده خشک) بود. با در نظر داشتن صفات تولیدی و فیتوشیمایی، رشت و ساری (شمال کشور) مناطق مناسبی برای کشت و تولید گیاه پریلا ارغوانی می‌باشند.

کلیدواژه‌ها

اسانس؛ رزمارینیک اسید؛ فنل و فلاونوئید کل؛ گیاهان دارویی

مراجع

Akbrian, A., Rahimmalek, M., Sabzalian, M., and Saeidi, G., 2017. Assessment of phytochemical, morphological and antioxidant variation of bilehar (Dorema aucheri) populations cultivated in different environmental conditions. JMP 2(62): 120-135. (In Persian with English Summary)

Ciocarlan, N., Sirbu, T., Stefanache, C., Ghendov, V., Necula, R., and Grigoras, V., 2014. Biological and phytochemical research on Perilla frutisence var. purputascens (HAYATA) H.W.LI in Republic of Moldova. Fiziologia şi Biochimia Plantelo. Nr. 2(323)

Cseke, L.J., Kirakosyan, A., Kaufman, P.B., Warber, S.L., Duke, J.A., and Brielmann, H.L., 2006. Natural Products from Plants 2^th Ed. CRC Press, Florida, 569 p.

Echeverrigaray, S., Fracaro, F., Dos-Santos, A.C.A., Paroul, N., Wasum, R., and Serafini, L.A., 2003. Essential oil composition of south Brazilian populations of Cunila galioides and its relation with the geographic distribution. Biochemical Systematics and Ecology 31: 467–475. https://doi.org/10.1016/S0305-1978(02)00175-8.

Esmaeili, H., Hadian, J., Mirjalili, M.H., and Rezadoost, H., 2016. Evaluation of some morphological, phytochemical and ecological characteristics of different populations of Satureja rechingeri Jamzad. Journal of Range and Watershed Management 69(1): 1-12. (In Persian with English Summary). https://doi.org/10.22059/jrwm.2016.61729

Feng, L.J., Yu, C.H., Ying, K.J., Hua, J., and Dai, X.Y., 2011. Hypolipidemic and antioxidant effects of total flavonoids of Perilla Frutescens leaves in hyperlipidemia rats induced by high-fat diet. Food Research International 44(1): 404-409.‏ https://doi.org/10.1016/j.foodres.2010.09.035

Gairola, S., Shariff, N., Bhate, A., and Prakash Kola, C., 2010. Influence of climate change on production of secondary chemicals in high altitude medicinal plants. Journal of Medicinal Plant Research 1825-1829. DOI: 10.5897/JMPR10.354.

Ghane, M.R., and Ghajar Sepanlu, M., 2018. Investigation of morphological, yield and medicinal properties of Perilla (Perilla frutscens) under chemical, biological and organic fertilizers in Mashhad Ardehal and Sensen regions of Kashan, Journal of Plant Production 25(2): 99-117. (In Persian with English Summary). https://dx.doi.org/10.22069/jopp.2018.13168.2189

Ghasemi Pirbalouti, A., Hashemi, M., Ghahfarokhi, F.T., 2013. Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops and Products 48: 43–48. https://doi.org/10.1016/j.indcrop.2013.04.004

Ghorbani, R., 2003. Organic agriculture and food production: Ecological, environmental, food safety and nutritional quality issues. Sociology, Organic Farming, Climate Change and Soil Science. Springer, Dordrecht p. 77-107.

Guan, Z., Li, S., Lin, Z., Yang, R., Zhao, Y., Liu, J., Yang, S., and Chen, A., 2014. Identification and quantification of phenolic compounds from the seed and pomace of Perilla frutescens using HPLC/PDA and HPLC-ESI/QTOF/MS/MS. Phytochemical Analysis. 25: 508-513. https://doi.org/10.1002/pca.2521

Ha, T.H., Lee, J.H., Lee, M.H., Lee, B.W., Kwon, H.S., and Park, C.H., 2012. Isolation and identification of phenolic compounds from the seeds of Perilla frutescens (L.) and their inhibitory activities against a-glucosidase and aldose reductase. Food Chemistry 135: 1317–1403. https://doi.org/10.1016/j.foodchem.2012.05.104

Hemati, K.H., Omidbeigi, R., and Bashiri Sadr, Z., 2003. Effect of climate and harvest time on the qualitative and qualitative characteristics of flavonoids of citrus varieties. Ph.D. Dissertation Tarbiat Modares University, Tehran, Iran.

Hodisan, T., Socaciu, C., Ropan, I., and Neamtu, G., 1997. Carotenoid composition of Rosa canina fruits determined by thin layer chromatography and high-performance liquid chromatography. Pharmaceutical and Biomedical Analysis 16(3): 521-528. https://doi.org/10.1016/S0731-7085(97)00099-X

Horwath, A.B., Grayer, R.J., Keith-Lucas, D.M., and Simmonds, M.S., 2008. Chemical characterization of wild populations of thymus from different climatic regions in southeast Spain. Biochemical Systematics and Ecology 36: 117–133. https://doi.org/10.1016/j.bse.2007.08.009

Jakola, L., and Hohtola, A., 2010. Effect of latitude on flavonoid biosynthesis in plants. Plant, Cell and Environmental 33(8): 1239-1247. https://doi.org/10.1111/j.1365-3040.2010.02154.x

Lee, J.H., Park, K.H., Lee, M.H., Kim, H.T., Seo, W.D., Kim, J.Y., and Ha, T.J., 2013. Identification, characterization, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars. Food Chemistry 136: 843–852. https://doi.org/10.1016/j.foodchem.2012.08.057

Mazandarani, M., Makari, S., and Bajian, G.R., 2011. Evaluation of phytochemical and antioxidant activity in different parts of Herscleum gorganicum Rech.F. in Golestan province, North of Iran. Iranian Journal Plant Physiology 2(2): 381-388.  https://doi.org 10.30495/IJPP.2012.540771

Meng, L., Lozano, Y., Bombarda, I., Gaydou, E., Li, B., 2006. Anthocyanin and flavonoid production from Perilla frutescens: pilot plant scale processing including cross-flow microfiltration and reverse osmosis. Journal Agricultural Food Chemistry. 54: 4297–4303. https://doi.org/10.1021/jf0604079

Meng, L., Lozano, Y., Bombarda, I., Gaydou, E. M., and Li, B., 2009. Polyphenol extraction from eight Perilla frutescens cultivars. Comptes Rendus Chimie 12(5): 602–611. https://doi.org/10.1016/j.crci.2008.04.011

Mirazadi, Z., Pilehvar, B., Meshkat Alsadat, M.H., and Karamian, R., 2012. Site quality and Essential oil composition of Myrtus Communis L. (case study: Cham moord site in Lorestan province). Journal of Agricultural Biotechnology 3(2): 71-79. (In Persian with English Summary)

Munoz-Bertomeu, J., Arrillaga, I., and Segura, J., 2007. Essential oil variation within and among natural populations of Lavandula latifolia and its relation to their ecological areas. Biochemical Systematics and Ecology 35: 479–488. https://doi.org/10.1016/j.bse.2007.03.006

Nooshkam, A., Mumivand, H., Hadian, J., Alemardan, A., and Morshedloo, M.R., 2017. Drug yield and essential oil and carvacrol contents of two species of Satureja (S. khuzistanica Jamzad and S. rechingeri Jamzad) cultivated in two different locations. Journal of Applied Research on Medicinal and Aromatic Plants 6, 126-130. (In Persian with English Summary). https://doi.org/10.1016/j.jarmap.2017.04.002

Parsafar, S., 2017. Evaluation of heterogeneity, growth and yield of some cultivars and populations of Solidago virgaurea L. M.Sc. Thesis, Shahid Beheshti University, Iran. (In Persian with English Summary)

Russo, A., Formisano, C., Rigano, D., Senatore, F., Delfine, S., Cardile, V., and Bruno, M., 2013. Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food and Chemical Toxicology 55: 42–47. https://doi.org/10.1016/j.fct.2012.12.036.

Samidha, K., Vrushali, K., and Vijaya, P., 2014. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. Journal of Applied Pharmaceutical Science 4(09): 061-065. http://dx.doi.org/10.7324/JAPS.2014.40911.

Sharma, R., Samant, S., Sharma, P., and Devi, S., 2012. Evaluation of antioxidant activity of Withania somnifera leaves growing in natural habitats of North-West Himalaya, India. Journal of Medicinal plants Research 6: 657-661. https://doi.org/10.5897/JMPR11.257.

Shi Jun, M., and Ju Kyong, L., 2017. Morphological variation of two cultivated types of perilla crop from different areas of China. Horticultural Science and Technology 35(4): 510-522.

Srivastava, A.R., Banerjee, A., Jacob, S., and Dunning, J., 2007. Should patients undergoing coronary artery bypass grafting with mild to moderate ischaemic mitral regurgitation also undergo mitral valve repair or replacement?. Interactive Cardiovascular and Thoracic Surgery 6(4): 538-546. https://doi.org/10.1510/icvts.2007.157891.

Telci, I., Kacar, O., Bayram, E., Arabac, O., Demirtas, I., Yilmaz, G., and Göksu, E., 2011. The effect of ecological conditions on yield and quality traits of selected peppermint (Mentha piperita L.) clones. Industrial Crops and Products 34: 1193–1197. https://doi.org/10.1016/j.indcrop.2011.04.010.

Valizadeh, J., Bagheri, A., and Mirjalili, M.H., 2015. Phytochemical investigation of Withania coagulans (Stocks) dunal in natural habits of Iran. Iranian Journal of Medicinal and Aromatics Plants 31: 406-417.

Wojdylo, A., Oszmianski, J., and Czemerys, R., 2007. Antioxidant activity and phenolic compound in 32 selected herbs. Food Chemistry 105(3): 940-949. https://doi.org/10.1016/j.foodchem.2007.04.038.

Yavari, A.R., Nazeri, V., Sefidkon, F., and Hassani, M.E., 2010. Evaluation of some ecological factors, morphological traits and essential oil productivity of Thymus migricus Klokov and Desj.-Shost. Iranian Journal of Medicinal and Aromatic Plants 26(2): 227-238. (In Persian with English Summary). http://dx.doi.org/10.22092/ijmapr.2015.11933

Yazdani, D., Rezazadeh, S., and Shahnazi, C., 2002. A review of the Papaver somniferum plant.  Journal of Medicinal Plants 5: 1-12. (In Persian with English Summary)

Zargari, A., 1992. Medicinal Plants, volume 1, Tehran University Press, Iran. 486. (In Persian)

Zhang, Z.H., VanCoillie, F.M., De Clercq, E., Ou, X., and De Wulf, R., 2013. Mountain vegetation change quantification using surface landscape metrics in Lancang watershed, China. Ecological Indicators 31: 49-58. https://doi.org/10.1016/j.ecolind.2012.11.013