Synthesis and study of the pH effect on the antibacterial activity of ZnSe nanoparticles against the Escherichia coli and Pseudomonas aeruginosa gram-negative bacteria by using disc-diffusion bioassay

مهندسی متالورژی و مواد

Article 2, Volume 33, Issue 1 - Serial Number 25, May 2021, Pages 15-24   

Document Type: Original Articles

DOI: 10.22067/jmme.2021.58903.0

Authors

Dariush Souri^{* 1}; Saeid Mohaghegh Nejad²; Mehdi Ghabooli³

¹Faculty of Science, Department of Physics, Malayer University.

²Department of Physics, Malayer University

³Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University.

Abstract

In the present research, ZnSe nanoparticles were synthesized by aqueous method at pHs of 8, 10.2, 11.2 and 12.2 and were grown by microwave irradiation at the times of 0 and 6 minutes. In the synthesis process, Se and Zn ions sources were added to each other at the presence of thioglycolic acid as capping agent . Antibacterial properties of these nanoparticles have been carried out by using disc diffusion method (in solid Muller-Hinton-Agar culture) against the Escherichia coli and Pseudomonas aeruginosa gram-negative bacteria in the loading volume of 140 µL of nanoparticles. Generally, results showed that by increasing the radiation time from 0 to 6 min, bacterial growth inhibition zone increased for Pseudomonas aeruginosa bacteria in compare with Escherichia coli bacteria; the highest antibacterial activity is devoted to the cases of pH=10.2 and 11.2, and these samples can be introduced as new agents in the field of nano-biotechnology applications.

Keywords

Gram-negative bacteria; ZnSe nanoparticles; microwave irradiation; disc-diffusion method; antibacterial property

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