Effects of Biochar with Vitamin C as Replacement of Dietary Mineral Supplements on Performance and Egg Shell Quality of Laying Hens

پژوهشهای علوم دامی ایران

Article 7, Volume 14, Issue 3 - Serial Number 51, September 2022, Pages 399-412   

Document Type: Research Articles

DOI: 10.22067/ijasr.2021.70097.1021

Authors

Fatemeh Ahmadi; Mohsen Afsharmanesh^* ; Mohammad salarmoini

Department of Animal Science. College of Agriculture. Shahid Bahonar University of Kerman. Kerman. Iran

Abstract

Introduction: Humate material was used as feed additives in poultry nutrition. The biochar as a useful fertilizer in agriculture industry is a humate material that increases growth of plants. This fertilizer can as an additive be use in the poultry and animals feed, due to has many minerals material and two organic acid (humic and fulvic) in its compound. The reduce cost of diet in poultry industry for egg production, is an important goal. The biochar as a dietary additive is easily derived from burn organic material and agriculture byproduct. In recent years, several studies have been conducted to determine the effect of humic acid as an additive on performance poultry. In a study, dietary supplementation of laying hens with humic acid improves the performance and egg shape index. Investigating the effect of biochar as replacement of dietary mineral supplements on performance and egg shell quality of laying hens is rare. One of the properties of vitamin C (VC) is chelating properties, and with this property, it increases the intestinal absorption of minerals in birds. Therefore, this study was conducted to investigate the effects of biochar (as a humate material) in five levels and vitamin C in two levels as replacement of dietary mineral supplements on performance and egg shell quality of laying hens.                       
Materials and Methods: The current experiment was conducted to evaluate the effects of biochar with vitamin C as replacement of mineral supplement in diet of laying hen. A total of 400 laying hens of Bovans strain, were randomly assigned to 10 experimental groups in a 5×2 factorial treatment arrangement with five replications and eight laying hens in each replicate during three 30-day periods. Factors tested included biochar levels (0, 25, 50, 75, and 100% replacement with mineral supplements of diet) and vitamin C levels (0 and 100 mg/kg of diet). The feed intake, feed conversion ratio and daily weight gain were determined. Records of the feed intake were taken on by daily basis. Feed conversion ratio was calculated as grams of feed consumed to grams of egg mass. To determine egg shell quality characteristics, the shape index was calculated by using egg height and egg width. The egg specific gravity was evaluated by floating eggs in soluble of water and salt with different density. The egg breaking strength was measured by resistance meter.
Results and Discussion: Replacing biochar levels from 25 to 100% mineral supplement of diet, with and without vitamin C, had no effect on performance characteristics (feed intake, feed conversion ratio and daily weight gain) and egg shell quality traits (egg shape index, egg specific gravity and egg breaking strength) in total of period of the experiment (50 to 61 weeks of age). The levels of 25 and 50% biochar, plus vitamin C in diet decreased feed intake in the first period. Whereas, the level of 75% biochar, plus vitamin C in diet increased feed intake in this period. The egg shape index with the addition of vitamin C in diet decreased in the third period. The reason of no significant effect of biochar on performance and egg shell quality can be due to the different sources and amounts of biochar in poultry diet. The reason of no significant effect of vitamin C on performance and egg shell quality in laying hens can be due to the bird environmental temperature. The use of vitamin C in diet of laying hens under heat stress improves their performance. Whereas, this study was performed in normal environmental temperature. The second reason is the chelating properties of vitamin C,  that can be increased absorption minerals in birds intestinal. But due to advanced digestive tract of laying hens, maybe decrease its properties.   
Conclusion: The results of present study showed that biochar across all levels had similar cost with control group, and it had no effect on egg shell quality and performance of laying hens. There was no significant interaction between biochar and vitamin C regrding to performance, egg shell quality traits and cost of diet during 50 to 61 weeks of age, too. Therefore, biochar can be completely replaced with dietary mineral supplements of laying hens due to its low cost, high availability, easy production without adverse effects on performance and egg quality traits.

Keywords

Biochar; Egg shell quality; Humic acid; Laying hen; Vitamin C

References

1. Ahmadi, M., & Karimi Torshizi, A. )2015(. Evaluation of vermicompost supplementation in Japanese quail diet. Iranian Journal of Animal Science Research, 7(1), 66-76. (In Persian).
2. Ajuwon, K. M., Matanmi, O., & Daniyan, O. (2002). Effect of water sources and ascorbic supplementation on egg quality and production parameters of laying hens. Livestock Research for Rural Development, 14, 6.       
3. Arif, M., Rehman, A., & Saeed, M. (2016). Impacts of dietary humic acid supplementation on growth performance, some blood metabolites and carcass traits of broiler chicks. Indian Journal of Animal Sciences, 86(9), 1073–1078.
4. Avci, M., Denek, N., & Kaplan, O. (2007). Effects of humic acid at different levels on growth performance, carcass yields and some biochemical parameters of quails. Journal of Animal Veterinary Advances, 6, 1-4.
5. Bailey, C. , White, K. E., & Donke, S. L. (1996). Evaluation of menefeehumate TM on the performance of broilers. Poultry Science, 75, 84.
6. Beesley, L., & Dickinson, N. (2011). Carbon and trace element fluxes in the pore water of an urban soil following greenwaste compost, woody and biochar amendments, inoculated with the earthworm lumbricus terrestris. Soil Biology and Biochemistry, 43(1), 188-196. https://doi.org/10.1016/j.soilbio.2010.09.035.
7. Ciftci, M., Ertas, O. N., & Guler, T. (2005). Effects of vitamin E and vitamin C dietary supplementation on egg production and egg quality of laying hens exposed to achronic heat stress. Revue Medicina Veterinarni, 156(2), 107-111.
8. Coskun, B., Inal, F., Erganis, O., Kuyucuoglu, Y., Ok, Ü., Çelik, I., Tiftik, A. M., & Kurtoglu, F. (1998). The effects of various level of ascorbic acid on the immunity and egg yield of laying hens. Turkish Journal Veterinarni Animal Science, 22, 29-35.
9. Dayyani, N., BeykiBandarAbadi, M., & Amirabadi Farhani, A. (2013). Cheated minerals in animal nutrition. International Journal of Advanced Biological and Biomedical Research, 1(11), 1387-1391.
10. Elalfy, M., Adly, A. A., Ismail, E., & Elalfy, O. (2015). June. Efficacy and safety of vitamin C as an adjuvant to iron chelation therapy in young patients with B-thalassemia major: A randomized prospective trial. In Haematologica (Vol. 100, pp. 131-131). Via giuseppe belli 4, 27100 pavia, italy: ferrata stortifoundation. https://doi.org/10.1111/ejh.12594.
11. Gerlach, H., & Schmidt, H. P. (2012). Biochar in poultry farming. Ithaka Journal, 1, 262–264.
12. Gross, W. (1988). Effect of ascorbic acid on antibody response of stressed and unstressed chickens. Avian Diseases, 32, 483-485.
13. Harms, R. H., Abdallah, A. G., & Slona, D. R. (1994). Error in measuring Hens. Annals of Animal Science, 15(3), 711-723.
14. Hayirli, A., Esenbuga, N., Macit, M., Lacin, E., Karaoglu, M., Karaca, H., & Yildiz, L. (2005). Nutrition practice to alleviate the adverse effects of stress on laying performance, metabolic profile and egg quality in peak producing hens. Journal of Animal Science, 18, 1310-1319.
15. Jaffar, G. H., & Blaha, J. (1996). Effect of ascorbic acid supplementation in drinking water on growth rate, feed consumption and feed efficiency of broiler chickens maintained under acute heat stress conditions. Zivocisna Vyroba, 41, 485-490.
16. Karaoglu, M., Macit, M., Esenbuga, N., Durdag, H., Turgut, L., & Bilgin, O. (2004). Effect of supplemental humate nat different levels on the growth performance, slaughter and carcasstraits of broilers. International Journal of Poultry Science, 3, 406-410.
17. Karimi Torshizi, M. A., Rahimi, S., Mojgani, N., Esmaeilkhanian, S., & Grimes, J. (2008). Screening of indigenous strains of lactic acid bacteria for development of a probiotic for Asian-Australian Journal of Animal Science, 21, 1495-1500. (In Persian).
18. Kaya, C. A., & Tuncer, S. D. (2009). The effects of humates on fattening performance, carcass quality and some blood parameters of broilers. Journal of Animal and Veterinary Advances, 8, 281-284.
19. Keshavarz, K. (1996). The effect of different levels of vitamin C and cholecalsiferol with adequate or marginal levels of dietary calcium on performance and egg shell quality of laying hens. Poultry Science, 75, 1227-1235. https://doi.org/10.3382/ps.0751227.
20. Lala, A. O., Kwelum, N. O., So, A. O. O., Ajao, A. O., & Degbenjo, A. A. A., (2017). Response of broiler chickens to varying dosage of humic acid in drinking water. National Animal Production Research Institute, 29(1), 288-294.
21. Lehmann, J., & Joseph, S. (Eds). (2009). Biochar for Environmental Management: Science and Technology. 416 p. Earthscan London and Sterling, VA.
22. Macit, M., Elebi, S. C., Senbuga, N. E., & Araca, H. K. (2009). Effects of dietary humate supplementation on performance, egg quality and egg yolk fatty acid composition in layers. Journal of the Science of Food and Agriculture, 89, 315-319. https://doi.org/10.1002/jsfa.3447.
23. Mc Dowell, L. R. (1989). Vitamins in animal nutrition comparative aspects to human nutrition Vitamin C. pages 365-387. Academic Press, London.
24. Mohammad Sadeghi, F. (2019). Study of substitutintg the mineral premixe diet with complex of humic material and its combination with probiotics on performance, intestinal morphology, immune response and meat quality of broilers. M.Sc. thesis. Shahid Bahonar University of Kerman, Iran, 228, 1-4.
25. Mohiti Asli, M., Hosseini, S. A., Lotfollahian, H., & Shariatmadari, F. (2007). Effect of probiotics, yeast, vitamin E and vitamin C supplements on performance and immune response of laying hen during high environmental temperature. International Journal of Poultry Science, 6(12), 895-900.
26. National Research Council, NRC. (1994). Nutrient requirements of poultry. 9^th National Academy Press. Washington, DC.
27. Newman, S., & Leeson, S. (1999). The effect of dietary supplementation with 1, 25 dihydroxycholecalsifrol or vitamin C on the characteristics of the tibia of older laying hens. Poultry Science, 78, 85-90. https://doi.org/10.1093/ps/78.1.85.
28. Nowaczewski, S., Kontetecka, H., Rosinski, A., Koberling, S., & Koronowski, P. (2010). Egg quality of Japanese quail depending on layer age and storage time. Folia Biologica, 58, 201-207. https://doi.org/10.3409/fb58_3-4.201-207.
29. Olobatoke, R. Y., & Mulugeta, S. D. (2011). Effect of dietary garlic powder on layer performance, fecal bacterial load, and egg quality. Poultry Science, 90(3), 665-670. https://doi.org/10.3382/ps.2010-00736.
30. Parks, C., Ferket, P. R., Thomas, L. N., & Grimes, J. L. (1986). Growth performance and immunity of turkeys fed high and low crude protein diets supplemented with Menefee humate. Poultry Science, 75, 138-143.
31. Rahmati, M. M. H., Saki, A. A., Zamani, P., Eskandarlo, A., & TahmasbPour, B. (2011). Effect of vitamin C on performance, egg characteristics and some blood biochemical parameters in laying hens. Iranian Journal of Animal Science Research, 3(3), 236-242.
32. Sahin, N., & Sahin, K. (2001). Optimal dietary concentrations of vitamin C and chromiumpicolinate for alleviating the effect of low ambient temperature (6.2°C) on egg production, some egg characteristics and nutrient digestibility in laying hens. Veterinarni Medicina, 46, 229- 236. https://doi.org/10.1002/jtra.10014.
33. Sahin, K., Sahin, N., Onderci, M., Gursu, M. F., & Issi, M. (2003). Vitamin C and E can alleviate negative effects of heat stress in Japanese quails. Food Agriculture and Environment, 2, 244-249.
34. Saki, A. A., Rahmati, M. M. H., Zamani, P., Zaboli, Kh., & Matin, H. R. H. (2010). Can vitamin C elevate laying hen performance, egg and plasma characteristics under normal environmental temperature? Italian Journal of Animal Science, 9, 3.
35. Siahpour, S., Karimi Torshizi, M. A., Shariatmadari, F., & Nick Nafs, F. (2010). Investigating the effect of mineral and vitamin supplementation time on growth and economic performance of broiler chickens. Journal of Veterinary Research, 65(1), 13-18. (In Persian).
36. Swiatkiewicz, S., Arczewska-Włosek, A., Krawczyk, J., Puchała, M., & Jozefiak, D. (2015). Dietary factors improving eggshell quality: An updated review with special emphasis on microelements and feed additives. World's Poultry Science Journal, 71, 83-94. https://doi.org/10.1017/S0043933915000082.
37. Yalçın, S., Ergün, A., Özsoy, B., Yalçın, S., Erol, H., & Onbaşılar, İ. (2006). The effects of dietary supplementation of L-carnitine and humic substances on performance, egg traits and blood parameters in laying hens. Asian-Aust. Journal Animal Science, 19(10), 1478-1483. https://doi.org/10.5713/ajas.2006.1478.
38. Yasar, S., Gokcimen, A., Altunas, I., Yonden, Z., & Petekkaya, E. (2002). Performance and ileal histomorphology of rats treated with humic acid preparations. Journal of Animal Physiology and Animal Nutrition, 86, 257-264. https://doi.org/10.1046/j.1439-0396.2002.00383.x
39. Yoruk, M., Gul, M., Hayirli, A., & Macit, M. (2004). The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens. Poultry Science Journal, 83, 84-88. https://doi.org/10.1093/ps/83.1.84.
40. Zhang, Y., Wang, N. J., Zhang, H. J., Wu, S. G., & Qi, H. G. (2017). Effect of dietary supplementation of organic or inorganic manganese on eggshell quality, ultrastructure, and components in laying hens. Poultry Science, 96, 2184-2193.