Prevalence assessment of Salmonella serovars in apparently healthy pet dogs in Tehran, Iran.

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 10 اردیبهشت 1401  

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2022.73966.1102

نویسندگان

Atuosa Akbari Khakrizi¹؛ Ramak Yahyaraeyat ²؛ Iradj Ashrafi Tamai¹؛ Babak Beikzadeh³؛ Taghi Zahraei Salehi¹

¹Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

²Department of Microbiology and Immunologu, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

³Department of Cell & Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, Iran.

چکیده

Salmonellosis is considered to be a zoonotic disease. Since pet care has been popular recently, the possibility of transmitting the disease through oral-fecal contact is unavoidable. On the other hand, excessive use of human antibiotics to treat animals resulted in the emergence of antibiotic-resistant Salmonella serotypes. This study aimed to assess the prevalence of bacteria and antibiotic resistance to select the appropriate antibiotic for disease control. In this study, the presence of Salmonella serovars in 256 pet dogs' fecal samples was investigated by enrichment and selective culture. Then, the existence of virulence and antibiotic resistance genes, as well as phenotypic antimicrobial resistance, were assessed. Of the total of 256 fecal samples, 21 sample (8.2%) of pet dogs were positive for Salmonella, including S. Typhimurium, S. Enteritidis, S. Infantis, and S. Senftenberg. Based on our findings, all serovars carried virulence genes invA, invF, sitC, fimA and S. Typhimurium resistant to Ampicillin (100%), Tetracycline (50%), Oxytetracycline (75%), Florfenicol (50%) and Lincospectin (100%). While S. Enteritidis, S. Infantis and S. Senftenberg were sensitive to Ampicillin, Amikacin, Gentamicin and Ciprofloxacin. S. Infantis was also sensitive to all antibiotics. In conclusion, our findings suggest that pet dogs are potential sources of Salmonella strains that carry resistance and virulence genes. Thus, healthy pet dogs could play an important role in human Salmonellosis.

کلیدواژه‌ها

antibiotic resistance gene؛ pet dog؛ Salmonella؛ virulence gene