Interaction of Central Kisspeptin with Melanocortin, GABAergic, Corticotrophin, and NPY Systems on Food Intake in Chickens

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 10 اردیبهشت 1401  

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2022.74653.1109

نویسندگان

Ahmadreza Kord¹؛ bita vazir ¹؛ Morteza Zendedel kheybari²؛ Vahab Babapour²؛ Ahmad Asghari³

¹Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, Iran

³Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده

Kisspeptin is a key component of reproduction that can directly affect food intake in mammals. There is evidence suggesting that the melanocortin, GABAergic, corticotrophin and neuropeptide Y (NPY), have a mediatory role in reward. Accordingly, in this study, a total of 10 experiments were carried out to investigate theinterplay between kisspeptin and these systems for control of food intake in neonatal layer-type chickens. In the first experiment, chickens were intracerebroventricular (ICV) injected with saline and Metastin (Kisspeptin 0.25, 50, and 1 nmol). In the second experiment, saline, Metastin (1 nmol), BIBP-3226 (NPY1 receptor antagonist, 1.25 nmol), and co-injection of Metastin + BIBP-3226 were injected. Experiments 3-10 were similar to experiment 1, except that chickens received BIIE 0246 (NPY2 receptor antagonist, 1.25 nmol), CGP71683A (NPY5 receptor antagonist, 50 μg), Picrotoxin (GABAA receptor antagonist, 1.25 nmol), CGP54626 (GABAB receptor antagonist, 21 µg), astressin-B (CRF1 / CRF2 receptor antagonist, 30 µg), Astressin2-B (CRF2 receptor antagonist, 30 µg), SHU9119 (MC3 / MC4 receptor antagonist, 0.5 nmol), and MCL0020 (MC3 / MC4 receptor antagonist, 0.5 nmol) instead of BIBP-3226. Food intake was subsequently assessed at 120 min after the injection. Based on the findings, Metastin (0.25, 50, and 1 nmol) enhanced food intake in a dose- dependent manner (P<0.05). Co-injection of the Metastin + BIBP-3226 inhibited Metastin-induced hyperphagia (P<0.05). As Metastin was co-administered with Picrotoxin, the hyperphagic effect of the Metastin was blocked (P<0.05). These results showed that the effect of kisspeptin on food intake might be mediated by NPY1 and GABAA receptors in chickens.

کلیدواژه‌ها

Kisspeptin؛ GABA؛ Melanocortin؛ NPY؛ Corticotropin؛ Dietary intake؛ Layer chicken