تاثیر نانوسیلیکون و باکتری‌های محرک رشد بر زیست‌توده، گره‌زایی و برخی صفات فیزیولوژیک خلر (Lathyrus sativus L.)

پژوهشهای زراعی ایران

مقاله 5، دوره 20، شماره 4 - شماره پیاپی 68، دی 1401، صفحه 435-449   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/jcesc.2022.75528.1149

نویسندگان

رئوف سیدشریفی^{* 1}؛ رضا سید شریفی²؛ حامد نریمانی³

¹گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی، اردبیل، ایران

²گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی، اردبیل، ایران

³دانشجوی دکترای فیزیولوژی گیاهان زراعی، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی، اردبیل، ایران

چکیده

خلر یک گیاه فراموش شده است که تولید آن در کشاورزی پایدار اهمیت دارد. از این‌رو به‌منظور بررسی تاثیر نانوسیلیکون و باکتری‌های محرک رشد بر زیست‌توده، گره‌زایی و برخی صفات فیزیولوژیک خلر، آزمایشی به‌‌صورت فاکتوریل در قالب طرح پایه بلوک‌ کامل تصادفی در سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 1400 اجرا شد. فاکتورهای آزمایشی شامل کاربرد کودهای زیستی در چهار سطح (عدم تلقیح به‌عنوان شاهد، تلقیح بذر با آزوسپریلیوم، سودوموناس، کاربرد توام آزوسپریلیوم و سودوموناس) و محلول‌پاشی نانوسیلیکون در چهار سطح (محلول‌پاشی با آب به‌عنوان شاهد و محلول‌پاشی 25، 50 و 75 میلی‌گرم در لیتر نانوسیلیکون) بود. نتایج نشان داد که کاربرد توام آزوسپریلیوم و سودوموناس و محلول‌پاشی 75 میلی‌گرم در لیتر نانوسیلیکون وزن و حجم ریشه (به‌ترتیب 4/40 و 9/41%)، تعداد گره‌های فعال (25/81%)، درصد گره‌های فعال (2/33%)، وزن خشک گره (4/37%)، شاخص کلروفیل (46%)، محتوای نسبی آب (3/46%)، هدایت روزنه‌ای (6/34%) و عملکرد کوانتومی (1/34%) را نسبت به شرایط عدم کاربرد باکتری‌های محرک رشد و نانوسیلیکون، افزایش داد. همچنین بیش‌ترین درصد پروتئین برگ و ساقه (4/23 و 7/12%) و زیست‌توده کل (7/34%) در کاربرد توام آزوسپریلیوم با سودوموناس و محلول‌پاشی 75 میلی‌گرم در لیتر نانوسیلیکون نسبت به شرایط عدم کاربرد باکتری‌های محرک رشد و نانوسیلیکون به‌دست آمد. به‌نظر می‌رسد کاربرد باکتری‌های محرک رشد و نانوسیلیکون می‌تواند زیست‌توده کل خلر را به‌واسطه گره‌زایی و بهبود صفات فیزیولوژیکی افزایش دهد.

کلیدواژه‌ها

آزوسپریلیوم؛ سودوموناس؛ عملکرد کوانتومی؛ گره‌های فعال؛ محتوای نسبی آب

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