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Molecular detection and phylogenetic analysis of Bovicola caprae in West and Northwest of Iran based on the marker of cytochrome oxidase 1 | ||
Iranian Journal of Veterinary Science and Technology | ||
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 11 تیر 1401 | ||
نوع مقاله: Research Article | ||
شناسه دیجیتال (DOI): 10.22067/ijvst.2022.76724.1148 | ||
نویسندگان | ||
Mousa Tavassoli ![]() ![]() | ||
1Urmia University | ||
2Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
3Department of Food Control and hygiene, Faculty of Veterinary Medicine, Urmia University | ||
چکیده | ||
Abstract Lice are permanent, obligate ectoparasites for birds and mammals. Bovicola caprae cause hypersensitivity, irritation, dermatitis, anemia, lower weight gain, and lower productivity in goats. This study was conducted to molecular investigate B. caprae based on the mitochondrial genome in Western and Northwest of Iran. A total of 1017 samples of chewing lice collected from 10 cities of five provinces were identified using diagnostic keys. After DNA extraction and PCR, samples were sent for sequencing. Morphological results were consistent with molecular examinations. Nucleotide sequencing of samples isolated from different cities based on mitochondrial genome showed 100% intraspecific similarity. The sequences of B. caprae isolated in this study appeared in a branch next to the Canadian and Chinese samples in the phylogenetic tree with more than 90% similarity. The results of mitochondrial gene analysis in this study showed that this fragment is useful for showing intraspecific similarity, species and genus differentiation of B. caprae. | ||
کلیدواژهها | ||
cox1؛ lice؛ molecular study؛ Iran | ||
آمار تعداد مشاهده مقاله: 685 |