Protective effect of abscisic Acid in a spinal cord injury model mediated by suppressed neuroinflammation

Iranian Journal of Veterinary Science and Technology

دوره 14، شماره 4 - شماره پیاپی 29، بهمن 2022، صفحه 42-51   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2022.75655.1127

نویسندگان

Maryam Rezaeezadeh Roukerd¹؛ Sahel Motaghi^{* 1}؛ Balal Sadeghi²؛ Mehdi Abbasnejad³

¹Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

²Food, Hygiene and Public Health Department, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

³Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.

چکیده

Abscisic acid (ABA) is a phytohormone with modulatory roles. The anti-inflammatory effect of this hormone has been reported on different animal tissues. Immediately after spinal cord injury (SCI), neuroinflammation causes neuropathic pain and locomotor impairments. We investigated the impacts of ABA as an anti-inflammatory substance on an acute SCI model. The weight-drop contusion injury model was applied for inducing SCI in rats. The solvent, ABA (10, 15 μg/rat, IT), and MP (30 mg/kg, IP) were administered after injury. For the evaluation of proinflammatory gene expression, a real-time polymerase chain reaction was applied for the two inflammation markers tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Moreover, the tail-flick and Basso, Beattie, Bresnahan (BBB) tests were performed to determine the effects of ABA on the neuropathic pain and locomotor function in the chronic phase of injury, respectively. Our data showed that ABA reduced the gene expression of TNF-α and IL-1β in the spinal cord of injured rats. It also increased the latency response to nociceptive thermal stimuli and improved locomotor function. Our findings showed the anti-inflammatory impacts of ABA in improving neuropathic pain and locomotor functional recovery after SCI.

کلیدواژه‌ها

Abscisic acid؛ neuroinflammation؛ neuropathic pain؛ spinal cord injury

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