Effect of Winter Foliar Application of Urea on Changes in Leaf and Node Polyamines, Yield and Some Characteristics of Reproductive of Kinnow Mandarin Trees

علوم باغبانی

Article 13, Volume 37, Issue 2 - Serial Number 58, August 2023, Pages 467-479   

Document Type: Research Article

DOI: 10.22067/jhs.2022.77067.1176

Authors

Fatemeh Karamnezhad; Norollah Moallemi^* ; Esmaeil Khaleghi

Department of Horticultural Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Introduction
Nitrogen plays an important role in the uniformity and quality of citrus trees. Several studies previously reported that a low level of nitrogen in citrus trees is generally caused a reduction in yield and fruit quality (Aziz, 1997; Khan et al., 2009). In this regard, applying urea is recommended as the most suitable form of nitrogen for foliar application. The polyamines are included; putrescine, spermidine, and spermine which have been considered as plant growth regulators (Alcazar et al., 2010; Khezri et al., 2010). The role of nitrogen in vegetative and reproductive growth and yield, as well as the correlation between polyamines, flower induction and fruit set in other plants, were proved in previous studies. In this regard, the results of the current study will increase our understanding about the role of polyamines in the morphology of the tree and also the mechanism of regulating the alternate bearing of Kinnow mandarin leading to an approach method to address this problem.
 
Materials and Methods
To conduct this study a 17-year-old uniform of Kinnow Mandarin (Citrus reticulate Blanco) grafted onto Bitter orange (Citrus aurantium) rootstock, which grown in a commercial orchard, located in Dezful (Khuzestan Province in Iran). For sampling, the branches which possess leaves and nodes were selected from four sides of each tree, then leaves and nodes were collected at three different time points (one, three, and five weeks post-treatment). Samples were immediately frozen in liquid nitrogen after excision and transported to the Physiology Laboratory of fruit trees within 2h for determining the N fractions and polyamines. The concentration of N in dried leaves and nodes was determined using the colorimetry technique as described by Walling et al. (1989). The experiment was set up as a factorial treatment based on a randomized complete block design with three replications to investigate the effect of different concentrations of urea foliar application (0, 0.75%, 1.5%) on nitrogen and polyamines contents at different time points (Dec 22, Jan 5, Jan 20) followed by evaluating flower characteristics and yield in Kinnow mandarin plant. Data analysis including variance was carried out using MSTATC and SAS software. To compare the mean of polyamines and nitrogen in leaves and nodes, the cut-out method was used, and also for comparison of pistil dimensions, number of flowers, and yields, Duncan's multiple range test (DMRT) was performed.   
 
Results and Discussion
Results indicated that polyamines concentration and nitrogen decreased during the period of time and also, in most cases, polyamines concentration was lower in the nodes than the leaves. High levels of polyamines and nitrogen were obtained in leaves and nodes which were treated with the foliar application of 1.5 % urea concentration after Jan 20. The polyamines content in leaves and nodes was greatly dependent on the spraying time and urea concentration used. Spermine (Spm) was the dominant polyamines in leaves and nodes with the highest values of 44.01 nmol/gF.W, 34.41 nmol/gF.W, respectively. Regarding the fact that flower density was higher in trees that treated with urea concentration of 1.5 % after Jan 5 y than other treatments, however, their yield was lower than the trees that treated on Dec 22 with the same urea concentration. This was probably due to the flower abscission as well as the fruit abscission in June. The results of this study showed that the highest yield was obtained with 1.5 % urea concentration after foliar application on Dec 22 compared with other treatments. Regarding the fact that flower differentiation in mandarin occurs during the late January until late February (in Dezful conditions), it can be explained that the foliar application on Dec 22 was performed before differentiation and consequently, the trees have received their required nitrogen. Also, the low-temperature is considered as natural inducer of citrus flowering in the Dezful, likewise, foliar fertilizer application in winter along with the natural factor (low temperature) stimulates flowering in a larger number of buds resulting in increasing the flowering. As nitrogen promotes vegetative and reproductive growth, it can be said that increasing the nitrogen content of leaves followed by transfering to the nodes, increases the number of buds, especially reproductive buds, which leads to an increase in flowering and yield. According to this study, the foliar application of urea in winter with 1.5% concentration can increase yield in Kinnow mandarin trees. Therefore, polyamines can play an important physiological role in flower development of Kinnow mandarin.
 
Conclusion
In this study, we focused on the effect of the foliar application urea during winter on leaves and nodes of Kinnow mandarin trees and investigated the polyamines, Put, Spm, and Spd contents upon treatments. In conclusion, the application of foliar urea in winter resulted in the significant endogenous increase of polyamines and N in the leaves and nodes of Kinnow mandarin trees. Also, yield, flower density, and pistil diameter were increased by spraying urea. There is a possibility that free polyamines affect on physiological processes.

Keywords

Nitrogen; Putrescine; Spermidine; Spermine

References

1. Akbari Chermahini, S, Moallemi, N., & Zargar, A.S. (2010). Effect of winter foliar application of urea on some quantitative and qualitative characters of flower and fruit set of orange cv ‘Valencia’. The Society for Advancement of Horticulture, 12(1), 59-61.
2. Albrigo, L. (1999). Effects of foliar applications of urea or nutriphite on flowering and yields of Valencia orange trees. In Proceedings of the Florida State Horticultural Society(Vol. 112, pp. 1-4).
3. Alburquerque, N., Egea, J., Burgos, L., MartÍnez‐Romero, D., Valero, D., & Serrano, M. (2006). The influence of polyamines on apricot ovary development and fruit set. Annals of Applied Biology, 149(1), 27-33. https://doi.org/10.1111/j.1744-7348.2006.00067.x
4. Alcázar, R., Altabella, T., Marco, F., Bortolotti, C., Reymond, M., Koncz, C., & Tiburcio, A.F. (2010). Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta, 231, 1237-1249.
5. Antognoni, F., Fornalè, S., Grimmer, C., Komor, E., & Bagni, N. (1998). Long-distance translocation of polyamines in phloem and xylem of Ricinus communis plants. Planta, 204, 520-527.
6. Arias, M., Carbonell, J., & Agustí, M. (2005). Endogenous free polyamines and their role in fruit set of low and high parthenocarpic ability citrus cultivars. Journal of Plant Physiology, 162(8), 845-853. https://doi.org/10.1016/j.jplph.2005.01.011
7. Aziz, A. (2003). Spermidine and related‐metabolic inhibitors modulate sugar and amino acid levels in Vitis vinifera: possible relationships with initial fruitlet abscission. Journal of Experimental Botany, 54(381), 355-363. https://doi.org/10.1093/jxb/erg029
8. Benhamou, L., El-Otmani, M., Goumari, M., Talhi, M., Charif, L., Srairi, I., & Lovatt, C.J. (2004, February). The potential use of GA 3 and urea to manipulate flowering and reduce alternate-bearing pattern of the ‘Nour’Clementine mandarin. In  Intl. Soc. Citriculture(Vol. 2, pp. 479-483).
9. El-Otmani, M., Goumari, M., Srairi, I., Lbrek, A., Charif, L., & Lovatt, C.J. (2004). Heavy fruit load and late harvest inhibit flowering of the ‘Nour’Clementine mandarin. In Proceedings of the International Society of Citriculture(Vol. 2, pp. 525-527).
10. El-Yazal, M.A.S., & Rady, M.M. (2012). Changes in nitrogen and polyamines during breaking bud dormancy in “Anna” apple trees with foliar application of some compounds. Scientia Horticulturae, 136, 75-80. https://doi.org/10.1016/j.scienta.2012.01.001
11. Freie, R.L., & Young Jr, H.V. (1992). Florida agricultural statistics: citrus summary 1990-1991. Florida Agricultural Statistics Service, Orlando, FL.
12. Iglesias, D.J., Cercós, M., Colmenero-Flores, J.M., Naranjo, M.A., Ríos, G., Carrera, E., & Talon, M. (2007). Physiology of citrus fruiting, Brazilian Journal of Plant Physiology, 19(4), 333-362.
13. Karamnezhad, F., Moallemi, N., & Khaleghi, E. (2018). Effect of different levels of urea and different times of foliar application on biochemical characteristics in the Kinnow Mandarin trees, Iranian Journal of Horticultural Science and Technology, 19(3). (In Persian)
14. Khan, A.S., Malik, A.U., Pervez, M.A., Saleem, B.A., Rajwana, I.A., Shaheen, T., & Anwar, R. (2009). Foliar application of low-biuret urea and fruit canopy position in the tree influence the leaf nitrogen status and physico-chemical characteristics of Kinnow mandarin (Citrus reticulata Blanco). Pakistan Journal Botany,41(1), 73-85.
15. Khezri, M., Talaie, A., Javanshah, A., & Hadavi, F. (2010). Effect of exogenous application of free polyamines on physiological disorders and yield of ‘Kaleh-Ghoochi’pistachio shoots (Pistacia vera). Scientia Horticulturae, 125(3), 270-276. https://doi.org/10.1016/j.scienta.2010.03.014
16. Könrgshofer, H. (1989). Seasonal changes in polyamine content in different parts of juvenile spruce trees [Picea abies (L.) Karst.]. Journal of Plant Physiology, 134(6), 736-740.
17. Kytöviita, M.M., & Sarjala, T. (1997). Effects of defoliation and symbiosis on polyamine levels in pine and birch, Mycorrhiza, 7(2), 107-111. https://doi.org/10.1016/S0176-1617(89)80036-7
18. Lovatt, C.J., Zheng, Y., & Hake, K.D. (1988). Demonstration of a change in nitrogen metabolism influencing flower initiation in Citrus. Israel Journal of Botany, 37(2-4), 181-188.
19. Moore, G.A. (2001). Oranges and lemons: clues to the taxonomy of Citrus from molecular markers. TRENDS in Genetics, 17(9), 536-540. https://doi.org/10.1016/S0168-9525(01)02442-8
20. Pritsa, T.S., & Voyiatzis, D.G. (2004). Seasonal changes in polyamine content of vegetative and reproductive olive organs in relation to floral initiation, anthesis, and fruit development. Australian Journal of Agricultural Research, 55(10), 1039-1046.  https://doi.org/10.1071/AR04056
21. Pritsa, T.S., & Voyiatzis, D.G. (2005). Correlation of ovary and leaf spermidine and spermine content with the alternate bearing habit of olive. Journal of Plant Physiology, 162(11), 1284-1291. https://doi.org/10.1016/j.jplph.2005.01.017
22. Rabe, E., & Walt, H.P. (1993). Effects of pre-blossom low-biuret urea sprays on yield improvement in specific citrus cultivars. Citrus Journal, 3, 26-28.
23. Rao, G.N. (2007). Statistics for agricultural sciences. BS Publications.
24. Roussos, P.A., Pontikis, C.A., & Zoti, M.A. (2004). The role of free polyamines in the alternate-bearing of pistachio (Pistacia vera Pontikis). Trees, 18, 61-69.
25. Sharma, R.K., & Awasthl, R.P. (1990). Effect of growth regulators on crop regulation of kinnow (Citrus nobilis X Citrus deliciosa). Indian Journal of Horticulture, 47(2), 162-166.
26. Smith, T.A. (1985). Polyamines. Annual Review of Plant Physiology, 36(1), 117-143.
27. Sood, S., & Nagar, P.K. (2003). The effect of polyamines on leaf senescence in two diverse rose species. Plant Growth Regulation, 39, 155-160.
28. Valiente, J.I., & Albrigo, L.G. (2004). Flower bud induction of sweet orange trees [Citrus sinensis (L.) Osbeck]: effect of low temperatures, crop load, and bud age. Journal of the American Society for Horticultural Science, 129(2), 158-164. https://doi.org/10.21273/JASHS.129.2.0158
29. Walling, P.J. (1989). U.S. Patent No. 4,802,008. Washington, DC: U.S. Patent and Trademark Office.
30. Walter, H.J.P., & Geuns, J.M. (1987). High speed HPLC analysis of polyamines in plant tissues. Plant Physiology, 83(2), 232-234. https://doi.org/10.1104/pp.83.2.232