Molecular detection and phylogenetic analysis of Bovicola caprae in the west and northwest of Iran based on cytochrome oxidase 1 marker

Iranian Journal of Veterinary Science and Technology

دوره 14، شماره 3 - شماره پیاپی 28، آذر 2022، صفحه 11-20   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/ijvst.2022.76724.1148

نویسندگان

Khadijeh Seydigazafi¹؛ Mousa Tavassoli^{* 2}؛ Karim Mardani³

¹Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

²Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University,Urmia , Iran.

³Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia , Iran.

چکیده

Lice are permanent, obligate ectoparasites for birds and mammals. Bovicola caprae causes hypersensitivity, irritation, dermatitis, anemia, lower weight gain, and lower productivity in goats. This study was conducted to investigate B. caprae by molecular methods based on the mitochondrial genome in the West and Northwest of Iran. A total of 1017 samples of chewing lice collected from ten cities in five provinces were identified using diagnostic keys. After DNA extraction and PCR, samples were sent for sequencing. Morphological results were consistent with molecular examinations. Nucleotide sequencing of samples isolated from different cities based on mitochondrial genome showed 100% intraspecific similarity. The sequences of B. caprae isolated in this study appeared in a branch next to the Canadian and Chinese samples in the phylogenetic tree with more than 90% similarity. The results of mitochondrial gene analysis in the present research showed that this fragment is useful for showing intraspecific similarity and species and genus differentiation of B. caprae.

کلیدواژه‌ها

COX1؛ Iran؛ Lice؛ Molecular study

سایر فایل های مرتبط با مقاله

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1148_ persian abs-F.pdf

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