Study of lncRNA NEAT1 Gene Expression in Ovarian Cancer

Journal of Cell and Molecular Research

مقاله 8، دوره 14، شماره 1 - شماره پیاپی 27، دی 2022، صفحه 63-70   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2022.76959.1041

نویسندگان

Amin Moqadami؛ Mohammad Khalaj-Kondori^*

Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

چکیده

Long non-coding RNAs (lncRNAs) have recently emerged as effective regulatory agents in biological processes as well as in the formation of tumors. LncRNAs are important regulators of cell transformation and cancer progression. LncRNA NEAT1 is one of the most important lncRNAs, and its deregulation has been reported in a variety of human cancers. Ovarian cancer has an inverse relationship with the number of reported pregnancies and deliveries, while it has a direct relationship with infertility. This study aimed to investigate NEAT1 expression in ovarian cancer. A total of 140 tissue samples, including 70 ovarian tumors and 70 marginal samples, were included in the study. Total RNA was extracted using the RNXplus solution. The quality and quantity of the extracted RNAs were determined using gel electrophoresis and a NanoDrop device. The complementary DNA was synthesized by the reverse transcriptase enzyme, and quantitative reverse transcriptase PCR was used to quantify the expression of NEAT1. A comparison between the mean expression of NEAT1 in ovarian tumors and marginal samples showed an increase in NEAT1 expression in tumor tissue that was not statistically significant (P-value = 0.2). ROC curve analysis also showed that NEAT1 expression level might not be an informative biomarker for ovarian cancer.

کلیدواژه‌ها

NEAT1؛ Ovarian Cancer؛ qRT-PCR؛ Biomarker

مراجع

Basu A. K. (2018) DNA Damage, Mutagenesis and Cancer. International Journal of Molecular Sciences 19:970.

Chai Y., Liu J., Zhang Z. and Liu L. (2016) HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer. Cancer Med 5:1588-1598.

Chakravarty D., Sboner A., Nair S. S., Giannopoulou E., Li R., Hennig S., et al. (2014) The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer. Nat Commun 5:5383.

Chen S.-N., Chang R., Lin L.-T., Chern C.-U., Tsai H.-W., Wen Z.-H., et al. (2019) MicroRNA in Ovarian Cancer: Biology, Pathogenesis, and Therapeutic Opportunities. International Journal of Environmental Research and Public Health 16:1510.

Dong P., Xiong Y., Yue J., Hanley S. J. B., Kobayashi N., Todo Y., et al (2018) Long Non-coding RNA NEAT1: A Novel Target for Diagnosis and Therapy in Human Tumors. Front Genet 9:471.

Fotuhi S. N., Khalaj-Kondori M. and Karimi H. (2021) LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer. Journal of Cell and Molecular Research 12:49-55.

Ghafouri-Fard S. and Taheri M. (2019) Nuclear Enriched Abundant Transcript 1 (NEAT1): A long non-coding RNA with diverse functions in tumorigenesis. Biomedicine & Pharmacotherapy 111:51-59.

Ghasemi T., Khalaj-Kondori M., Hosseinpour Feizi M. A. and Asadi P. (2020) lncRNA-miRNA-mRNA interaction network for colorectal cancer; An in silico analysis. Comput Biol Chem 89:107370.

Ghasemi T., Khalaj-Kondori M., Hosseinpour Feizi M. A. and Asadi P. (2021a) Aberrant expression of lncRNAs SNHG6, TRPM2-AS1, MIR4435-2HG, and hypomethylation of TRPM2-AS1 promoter in colorectal cancer. Cell Biol Int 45:2464-2478.

Ghasemi T., Khalaj-Kondori M., Hosseinpour Feizi M. A. and Asadi P. (2021b) Long non-coding RNA AGAP2-AS1 is up regulated in colorectal cancer. Nucleosides Nucleotides Nucleic Acids 40:829-844.

Hu J., He G., Lan X., Zeng Z., Guan J., Ding Y.,  et al. (2018) Inhibition of ATG12-mediated autophagy by miR-214 enhances radiosensitivity in colorectal cancer. Oncogenesis 7:1-12.

Jarroux J., Morillon A. and Pinskaya M. 2017. History, Discovery, and Classification of lncRNAs. In Long Non Coding RNA Biology. M.R.S. Rao, editor. Springer Singapore, Singapore. 1-46.

Jin Y., Feng S. J., Qiu S., Shao N. and Zheng J. H. (2017) LncRNA MALAT1 promotes proliferation and metastasis in epithelial ovarian cancer via the PI3K-AKT pathway. Eur Rev Med Pharmacol Sci 21:3176-3184.

Karnezis A. N., Cho K. R., Gilks C. B., Pearce C. L. and Huntsman D. G. (2017) The disparate origins of ovarian cancers: pathogenesis and prevention strategies. Nature Reviews Cancer 17:65-74.

Khajehdehi M., Khalaj-Kondori M., Ghasemi T., Jahanghiri B. and Damaghi M. (2021) Long Noncoding RNAs in Gastrointestinal Cancer: Tumor Suppression Versus Tumor Promotion. Dig Dis Sci 66:381-397.

Khajehdehi M., Khalaj-Kondori M. and Hosseinpour Feizi M. A. (2022) Expression profiling of cancer-related long non-coding RNAs revealed upregulation and biomarker potential of HAR1B and JPX in colorectal cancer. Mol Biol Rep 49:6075-6084.

Khodayi-Shahrak M., Khalaj-Kondori M., Hosseinpour Feizi M. A. and Talebi M. (2022) Insights into the mechanisms of non-coding RNAs' implication in the pathogenesis of Alzheimer's disease. Excli j 21:921-940.

Khodayi M., Khalaj-Kondori M., Hoseinpour Feizi M. A., Jabarpour Bonyadi M. and Talebi M. (2022) Plasma lncRNA profiling identified BC200 and NEAT1 lncRNAs as potential blood-based biomarkers for late-onset Alzheimer’s disease. Excli j 21:772-785.

Koshiyama M., Matsumura N. and Konishi I. (2014) Recent concepts of ovarian carcinogenesis: type I and type II. Biomed Res Int 2014:934261.

Li S., Li J., Chen C., Zhang R. and Wang K. (2018) Pan-cancer analysis of long non-coding RNA NEAT1 in various cancers. Genes & diseases 5:27-35.

Liu H., Yan X. and Yu J. (2020) Long noncoding RNA NEAT1/microRNA-125a axis predicts increased major adverse cardiac and cerebrovascular event risk independently in patients with unprotected left main coronary artery disease underwent coronary artery bypass grafting. J Clin Lab Anal 34:e23299.

Liu Y., Wang Y., Fu X. and Lu Z. (2018) Long non-coding RNA NEAT1 promoted ovarian cancer cells' metastasis through regulation of miR-382-3p/ROCK1 axial. Cancer Sci 109:2188-2198.

Lo P.-K., Zhang Y., Wolfson B., Gernapudi R., Yao Y., Duru N., et al. (2016) Dysregulation of the BRCA1/long non-coding RNA NEAT1 signaling axis contributes to breast tumorigenesis. Oncotarget 7:65067.

Matulonis U. A., Sood A. K., Fallowfield L., Howitt B. E., Sehouli J. and Karlan B. Y. (2016) Ovarian cancer. Nat Rev Dis Primers 2:16061.

Momenimovahed Z., Tiznobaik A., Taheri S. and Salehiniya H. (2019) Ovarian cancer in the world: epidemiology and risk factors. Int J Womens Health 11:287-299.

Motofei I. G. (2021) Biology of cancer; from cellular and molecular mechanisms to developmental processes and adaptation. Seminars in Cancer Biology.

Nandwani A., Rathore S. and Datta M. (2021) LncRNAs in cancer: Regulatory and therapeutic implications. Cancer Letters 501:162-171.

Ostovarpour M., Khalaj-Kondori M. and Ghasemi T. (2021) Correlation between expression levels of lncRNA FER1L4 and RB1 in patients with colorectal cancer. Mol Biol Rep 48:4581-4589.

Shu C., Yan D., Mo Y., Gu J., Shah N. and He J. (2018) Long noncoding RNA lncARSR promotes epithelial ovarian cancer cell proliferation and invasion by association with HuR and miR-200 family. Am J Cancer Res 8:981-992.

Siegel R. L., Miller K. D., Fuchs H. E. and Jemal A. (2022) Cancer statistics, 2022. CA: A Cancer Journal for Clinicians 72:7-33.

Sung H., Ferlay J., Siegel R. L., Laversanne M., Soerjomataram I., Jemal A., et al. (2021) Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians 71:209-249.

Thankachan S., Bhardwaj B. K., Venkatesh T. and Suresh P. S. (2021) Long Non-coding RNA NEAT1 as an Emerging Biomarker in Breast and Gynecologic Cancers: a Systematic Overview. Reproductive Sciences 28:2436-2447.

Torre L. A., Trabert B., DeSantis C. E., Miller K. D., Samimi G., Runowicz C. D., et al. (2018) Ovarian cancer statistics, 2018. CA Cancer J Clin 68:284-296.

34- Wang J.-y., Lu A.-q. and Chen L.-j. (2019) LncRNAs in ovarian cancer. Clinica Chimica Acta 490:17-27.

Wang X., Yang B., She Y. and Ye Y. (2018) The lncRNA TP73‐AS1 promotes ovarian cancer cell proliferation and metastasis via modulation of MMP2 and MMP9. Journal of cellular biochemistry 119:7790-7799.

West J. A., Davis C. P., Sunwoo H., Simon M. D., Sadreyev R. I., Wang P. I., Tolstorukov M. Y. and Kingston R. E. (2014) The long noncoding RNAs NEAT1 and MALAT1 bind active chromatin sites. Mol Cell 55:791-802.

Xi J., Feng J. and Zeng S. (2017) Long noncoding RNA lncBRM facilitates the proliferation, migration and invasion of ovarian cancer cells via upregulation of Sox4. American Journal of Cancer Research 7:2180.

Xu H., Sun X., Huang Y., Si Q. and Li M. (2020) Long non‑coding RNA NEAT1 modifies cell proliferation, colony formation, apoptosis, migration and invasion via the miR‑4500/BZW1 axis in ovarian cancer. Mol Med Rep 22:3347-3357.

Xu Q. F., Tang Y. X. and Wang X. (2018) LncRNA EBIC promoted proliferation, metastasis and cisplatin resistance of ovarian cancer cells and predicted poor survival in ovarian cancer patients. Eur Rev Med Pharmacol Sci 22:4440-4447.

Yang C., Li Z., Li Y., Xu R., Wang Y., Tian Y. and Chen W. (2017) Long non-coding RNA NEAT1 overexpression is associated with poor prognosis in cancer patients: a systematic review and meta-analysis. Oncotarget 8:2672-2680.

ZadehRashki N., Shahmohammadi Z., Damrodi Z., Boozarpour S., Negahdari A., Mansour Moshtaghi N., et al. (2022) LncRNAs as Regulators of the STAT3 Signaling Pathway in Cancer. Journal of Cell and Molecular Research 13:137-150.

Zaorsky N. G., Khunsriraksakul C., Acri S. L., Liu D. J., Ba D. M., Lin J. L., et al. (2021) Medical Service Use and Charges for Cancer Care in 2018 for Privately Insured Patients Younger Than 65 Years in the US. JAMA Network Open 4:e2127784-e2127784.

Zhang P., Wu W., Chen Q. and Chen M. (2019) Non-Coding RNAs and their Integrated Networks. J Integr Bioinform 16.

Zhang Y., Dun Y., Zhou S. and Huang X. H. (2017) LncRNA HOXD-AS1 promotes epithelial ovarian cancer cells proliferation and invasion by targeting miR-133a-3p and activating Wnt/β-catenin signaling pathway. Biomed Pharmacother 96:1216-1221.

Zhen L., Yun-Hui L., Hong-Yu D., Jun M. and Yi-Long Y. (2016) Long noncoding RNA NEAT1 promotes glioma pathogenesis by regulating miR-449b-5p/c-Met axis. Tumour Biol 37:673-683.