Morphometric and genetic characterization of Oestrus ovis in sheep in different climatic regions of Iran

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 28 آبان 1401   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2022.77084.1153

نویسندگان

Sepideh Rajabi؛ Mousa Tavassoli^* ؛ Bijan Esmaeilnejad

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

چکیده

Oestrosis is nasal myiasis, which results from the infestation of larvae of flies that belong to the genus Oestrus. Nasal discharges and sneezing are the most common clinical signs in infected animals. Myiasis larvae were collected from sheep in different climatic regions of Iran. Morphological identification was made on the larvae by the diagnostic keys. PCR studies that targeted amplifying the partial fragment (610 bp) of  the mitochondrial COI gene confirmed the species. Genetic distances was carried out in CO1 sequences, and a phylogenetic tree was drawn. Sequencing showed no difference in the partial COI gene among the Iranian isolates and had high similarity with the sequences of O. ovis isolates from Iraq, Bosnia and Herzegovina, and Croatia. The first molecular data set for O. ovis species were provided by this study, which gives a crucial database for the phylogenetic relationships assessment and the molecular identification of these parasites.

کلیدواژه‌ها

Oestrus ovis؛ Sheeps؛ COI؛ PCR؛ Phylogeny؛ Sequence