تأثیر مکمل ویتامین E جیره غذایی بر کیفیت‌ اسپرم تازه خروس‌های مادر گوشتی سویه راس 308 چالش یافته با تنش اکسیداتیو

پژوهشهای علوم دامی ایران

مقاله 6، دوره 15، شماره 2 - شماره پیاپی 54، تیر 1402، صفحه 211-224   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ijasr.2023.75961.1070

نویسندگان

ذبیح اله نعمتی^* ؛ نامدار کامرانی؛ محمد ستاری؛ امیر کریمی؛ مقصود بشارتی

گروه علوم دامی،دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، تبریز، ایران.

چکیده

هدف از انجام این آزمایش، بررسی اثر افزودن ویتامین E به جیره غذایی بر کیفیت اسپرم در خروس‌های تحت تنش اکسیداتیو بود. تعداد 18 قطعه از خروس گله مادر گوشتی سویه راس 308 در سن 28 هفتگی به‌صورت تصادفی در سه گروه آزمایشی شامل گروه شاهد، گروه دگزامتازون (تزریق زیر پوستی چهار میلی‌گرم دگزامتازون به‌ازای کیلوگرم وزن بدن) و گروه دگزامتازون دریافت‌کننده جیره مکمل شده با ۲۰۰ میلی‌گرم ویتامین E درکیلوگرم خوراک، اختصاص داده شد. هر گروه آزمایشی شامل شش پرنده بود. اسپرم­گیری از خروس‌ها با استفاده از روش ماساژ شکمی انجام و خصوصیات کیفی و وضعیت آنتی­اکسیدانی نمونه­های اسپرم به‌روش استاندارد ارزیابی شد. نتایج نشان­داد، القای تنش اکسیداتیو با تزریق دگزامتازون سبب افزایش مالون دی­آلدهید و کاهش یکپارچگی غشای پلاسمای، فعالیت آنزیم‌های گلوتاتیون پراکسیداز، سوپر­اکسید دیسموتاز و شاخص­های حرکتی اسپرم، در مقایسه با گروه شاهد شد. افزودن ویتامین E به جیره­غذایی خروس‌ها سبب بهبود اثرات منفی تنش اکسیداتیو بر شاخص­های حرکتی اسپرم، وضعیت آنتی‌اکسیدانی اسپرم (ظرفیت آنتی‌اکسیدانی و آنزیم‌های سوپراکسیداز و گلوتاتیون پراکسیداز)، قابلیت زنده‌مانی و یکپارچکی غشای اسپرم شد. می­توان نتیجه گرفت، گنجاندن ویتامین E در جیره غذایی می­تواند سبب بهبود خصوصیات حرکتی و وضعیت آنتی­اکسیدانی اسپرم در خروس‌های تحت تنش شود.

کلیدواژه‌ها

خروس مادر گوشتی؛ تنش اکسیداتیو؛ کیفیت اسپرم؛ ویتامین E

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