5-azacytidine increase tanshinone production in endophytic fungi of Salvia abrotanoides(Kar.) Sytsma

Journal of Cell and Molecular Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 بهمن 1401

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2023.79535.1058

نویسندگان

maryam varasteh-kojourian¹؛ Ali Ganjeali^{* 2}؛ Javad asili³؛ Saeid Malekzadeh-Shafaroudi⁴؛ Akram Taleghani⁵

¹Ferdowsi University of mashhad, Mashhad, Iran

²Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

³Pharmacognosi Department, Pharmacology School, Medical Science University of Mashhad, Mashhad, Iran

⁴Department of Crop Biotechnology and Breeding Faculty of Agriculture

⁵Chemistry Department, Science school, Gonbad university, Gonbad, Iran

چکیده

Endophytic fungi are often producing host plant secondary metabolites. Tanshinones are secondary metabolites of the Salvia genus which also produced by some endophytic fungi. Efficient secondary metabolite production in endophytic fungi drops significantly after sequential subcultures. 5-azacytidine (5-AC) is an analog of the naturally occurring pyrimidine nucleoside cytidine and a DNA methyltransferase inhibitor. In this relation, 5-AC is an effective tool to induce expression of silenced secondary metabolite genes in endophytic fungi. In this experiment we isolated 4 endophytic fungi from the roots of Salvia abrotanoides which produced tanshinone. Cryptotanshinone and tanshinon IIA were produced by Penicillium canescens, Penicillium nodositatum, and Penicillium pinophilum, while Paraphoma radicina only produced tanshinon IIA. The maximum amount of tanshinones was extracted from P. pinophilum culture with 130.826 mg cryptotanshinone /g of dry weight and also 50.155 mg Tanshinone IIA/g of dry weight. These amounts were significantly more than the tanshinones produced in plant roots (0.55 mg cryptotanshinone/g of dry weight, 1.3 mg Tanshinone IIA/g of dry weight). In third subculture, tanshinone production decreased significantly. 5-azacytidine as an epigenetic modifier retrieved tanshinone production in 3d subculture of P. pinophilum. Also, 5- azacytidine treatment made a big jump in Tanshinone IIA production in P. radicina (63.176 mg TIIA/g of dry weight) besides increasing Tanshinone IIA production in P. nodositatum cultures. This is the first report using 5- azacytidine to improve tanshinone production in endophytic fungi. Our results confirm that 5- azacytidine is an efficient, easy, and quick chemical to elicitate secondary metabolite production in endophytic fungi.

کلیدواژه‌ها

Endophytic fungi؛ Tanshinone؛ 5-Azacytidine؛ Salvia abrotanoides