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Mirzaei Aghjehgheshlagh, F., Nori, A., Navidshad, B., kaviani Feizi, L., Karamati jabehdar, S. (2023). Dietary Effects of Pomegranate Pomace Powder with or without Tannase Enzyme on Growth Performance and some Blood Parameters of Moghani Male Lambs. , 15(4), 475-488. doi: 10.22067/ijasr.2023.80199.1116
Farzad Mirzaei Aghjehgheshlagh; Ali Nori; Bahmam Navidshad; leila kaviani Feizi; Samira Karamati jabehdar. "Dietary Effects of Pomegranate Pomace Powder with or without Tannase Enzyme on Growth Performance and some Blood Parameters of Moghani Male Lambs". , 15, 4, 2023, 475-488. doi: 10.22067/ijasr.2023.80199.1116
Mirzaei Aghjehgheshlagh, F., Nori, A., Navidshad, B., kaviani Feizi, L., Karamati jabehdar, S. (2023). 'Dietary Effects of Pomegranate Pomace Powder with or without Tannase Enzyme on Growth Performance and some Blood Parameters of Moghani Male Lambs', , 15(4), pp. 475-488. doi: 10.22067/ijasr.2023.80199.1116
Mirzaei Aghjehgheshlagh, F., Nori, A., Navidshad, B., kaviani Feizi, L., Karamati jabehdar, S. Dietary Effects of Pomegranate Pomace Powder with or without Tannase Enzyme on Growth Performance and some Blood Parameters of Moghani Male Lambs. , 2023; 15(4): 475-488. doi: 10.22067/ijasr.2023.80199.1116

Dietary Effects of Pomegranate Pomace Powder with or without Tannase Enzyme on Growth Performance and some Blood Parameters of Moghani Male Lambs

پژوهشهای علوم دامی ایران
Article 1, Volume 15, Issue 4 - Serial Number 56, December 2023, Pages 475-488    PDF (780.75 K)
Document Type: Research Articles
DOI: 10.22067/ijasr.2023.80199.1116
Authors
Farzad Mirzaei Aghjehgheshlagh* 1; Ali Nori2; Bahmam Navidshad1; leila kaviani Feizi1; Samira Karamati jabehdar2
1Department of animal science, University of Mohaghegh Ardabili, Ardabil, Iran
2Department of Animal Science, Mohaghegh Ardabili University, Ardabil, Iran
Abstract
Introduction: One of the important by-products is the pomegranate pulps which is pulp left after pomegranate juice. The presence of significant amounts of biologically active compounds such as phenolic acid, flavonoids, and tannins in pomegranate fruit ensures its high nutritional value. Pomegranate peel is a part of the fruit that has very high antioxidant properties and contains high amounts of polyphenols such as tannins. There have been numerous reports of the negative effects of tannins on consumer animals. One of the compounds that can bind to tannins and reduce their harmful effects is the tannase enzyme. So, this study aimed to investigate the effects of using different levels of pomegranate pulp without or in combination with tannase enzyme in the diet of fattening lambs on performance, nutrient digestibility, and some blood parameters.
Materials and Methods: After preparing pomegranate pulp, drying it, and preparing tannase enzyme, 25 male Moghani lambs with an average weight of 30±2 kg and the average age is about 7 months, were used in four treatments in a completely randomized design. experimental treatments include 1- control, 2- 2.5% pomegranate pulp powder, 3- 5% pomegranate pulp powder, 4- 2.5% pomegranate pulp powder + 0.05 % dry matter of tannase enzyme 5- 5% pomegranate pulp powder + 0.05 % dry matter of tannase enzyme. During the experimental period, the performance parameters including feed intake, weight gain, and feed conversion ratio were measured. The concentration of blood parameters and the activity of liver enzymes containing ALP, ALT, and AST in the blood of lambs were determined. The Data obtained were analyzed using SAS (9/1) statistical software.
Results and Discussion: The experimental treatments demonstrated no significant impact on the weight gain and feed intake of the lambs. However, when examining the amount of feed consumption and daily feed consumption, higher values were observed during the second 30 days of the rearing period in comparison to the initial 30 days.While nutrient digestibility remained largely unaffected, a notable trend in the digestibility of dry matter was observed. Further comparisons between treatments revealed a higher digestibility of organic matter, neutral detergent fiber (NDF), acid detergent fiber (ADF), and ash in the group receiving 2.5% pomegranate pomace. These findings provide insights into the potential influence of pomegranate pomace supplementation on specific aspects of nutrient utilization in lamb diets. Fat in the treatment of 2.5% pomace with enzyme and digestibility of crude protein was higher in the treatment of 5% pomace with the enzyme. The comparison of different pomace levels showed that the use of high levels of pomegranate pomace (5%) increased blood glucose and the lowest blood urea concentration was obtained by feeding 5% pomace. The lowest concentrations of HDL and LDL in the blood also belonged to the control treatment. The use of tannase enzymes in the diet also increased the concentration of glucose parameters and decreased blood urea. The highest amount of glucose of the studied lambs were observed in the first thirty days of sampling. In the present study, the use of pomegranate pomace increased glucose in both rearing periods and decreased blood triglyceride levels in the second rearing period. Alanine phosphatase (ALP) enzyme activity was significant in the second period, the breeding period (P<0.05). The highest level of activity of this enzyme was observed in the blood of lambs fed with 2.5% of dung. The activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was not significant between sampling periods (P<0.05). Feeding different levels of pomegranate pomace powder with or without tannase enzyme had no significant effect on blood malondialdehyde concentration as an antioxidant index of the blood of fattening lambs. However, the comparison between the experimental treatments showed that the highest level of this index was found in the blood samples obtained from lambs fed with a diet containing 5% of pomegranate pomace powder + 0.05% of the dry matter of the tannase enzyme diet.
Conclusion: The inclusion of different levels of pomegranate pomace in the diet led to a significant difference in the activity of the alanine phosphatase enzyme among the experimental treatments (P<0.05) and increased blood glucose (P<0.05). None of the functional parameters, including weight gain, and feed consumption, were affected by the use of pomegranate pulp with or without tannase enzyme during the entire experimental period. However, the food conversion coefficient improved in the treatments containing pomegranate pomace with tannin enzyme. The highest amount of antioxidant index related to the diet containing 5% of pomegranate pomace powder + 0.05% of the diet's dry matter was tannase enzyme. Therefore, the level of 5% of pomegranate pomace along with the tannase enzyme increases animal health and improves animal growth performance.
 
Keywords
Blood parameters; Fattening lamb; Growth performance; Pomegranate pulp; Tannase
References
  1. Al-Dujaili, E., & Smail, N. (2012). Pomegranate juice intake enhances salivary testosterone dose and improves mood and wellbeing in healthy men and women. Endocrine Abstracts, 28,3-13.
  2. AOAC International. (2000). Official Methods of Analysis. 17th Ed, AOAC International, Gaithersburg, MD.
  3. Barry, T. N. (1985). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. British Journal of Nutrition, 54(1),211–217. http://dx.doi.org/1079/BJN19850106
  4. Broderick, G. A., Wallace, R. J., & Orskov, E. R. (1991). Control of rate and extent of protein degradation. In: Tsuda, T., Sasaki, Y., Kawashima, R. (Eds), Physiological Aspects of Digestion and Metabolism in Ruminants. Academic Press, Inc. San Diego, 541-592.
  5. Buccioni, A. Pauselli, M., Viti, C., Minieri, S., Pallara, G., & Roscini, V.. (2015). Milk fatty acid composition, rumen microbial population, and animal performances in response to diets rich in linoleic acid supplemented with chestnut or quebracho tannins in dairy ewes. Journal of Dairy Science, 98(2),1145–1156. http://dx.doi.org/3168/jds.2014-8651
  6. Chamorro, S., Viveros, A., Centeno, C., Romero, C., Arijia, I., & Brenes, A. (2013). Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks. Animal, 7(4), 555-561. http://dx.doi.org/1017/S1751731112001851
  7. Devatkal, S. K., & Naveena, B. (2010). Effect of salt, kinnow and pomegranatefruit by-product powders on color and oxidative stability of raw ground goat meat during refrigerated storage. Meat Science, 85(2),306-311. http://dx.doi.org/1016/j.meatsci.2010.01.019
  8. Emami, A., Ganjkhanlou, M., Fathi-Nasri, M. H., Zali, A., & Rashidi, L. (2015). Pomegranate seed pulp as a novel replacement of dietary cereal grains for kids. Small Ruminant Research, 123(2-3),238-245.  http://dx.doi.org/1016/j.smallrumres.2014.12.001
  9. Ghasemi, G. H., Fathi-Nasri, M. H., Modaresi, S., & Rashidi, L. (2016). The effect of replacing of some of cereal grains of diet with ensiled pomegranate seed pulp on dry matter intake and production performance of south Khorasan crossbred goats. Journal of Animal Science Researches, 2(1) 93-104. (In Persian).
  10. Getachew, G., Pittroff, W., Depeters, E. J., Putnam, D. H., Dandekar, A., & Goyal, S. (2008). Influence of tannic acid application on alfalfa hay: In vitro rumen fermentation, serum metabolites and nitrogen balance in sheep. Animal, 2(3), 381-390. http://dx.doi.org/1017/S1751731107001486
  11. Gil, M. I., Tomas-Barberan, F. A., Hess-Pierce, B., Holcroft, D. M., & Kader, A. A. (2000). Antioxidant activity of pomegranate juice and its relationshipwith phenolic composition and processing. Journal of Agricultural Food and Chemistry, 48(10), 4581-4589. http://dx.doi.org/1021/jf000404a
  12. Guo, C. J., Yang, J. J., Wei, J. Y., Li, Y. F., Xu, J., & Jiang, Y. G. (2003). Antioxidant activities of pulp, pulp and seed fractions of common fruits as deter-mined by FRAP assay. Nutritional Research, 23(12), 1719-1726. http://dx.doi.org/101016/j.nutres.2003.08.005
  13. Hagerman, A. E. & Robbins, C. T. (1987). Implications of soluble tannin protein complexes for tannin analysis and plant defense mechanisms. Journal of Chemistry and Ecology, 13(5), 1243-1247. http://dx.doi.org/1007/BF01020552
  14. Hervas, G., Frutos, P., Giraldez, F. J., Mantecon, A. R., & del Pino, M. C. A. (2003). Effect of different doses of quebracho tannins extract on rumen fermentation in ewes. Animal Feed Science Technology, 109(1-4), 65-78. http://dx.doi.org/1016/s0377-8401(03)00208-6
  15. Ibrahim, W. H., Habib, H. M., Chow, C. K., & Bruckner, G. G. (2008). Isoflavone-rich soy isolate reduces lipid peroxidation in mouse liver. International Journal for Vitamin and Nutrition Research, 78(45), 217-222. http://dx.doi.org/1024/0300-9831.78.45.217
  16. Jabbar naser, H. (2010). Importance of conversion industry waste and agricultural waste in livestock and poultry feed production. Livestock and Agro-Industry Magazine, 124,89-98. (In Persian).
  17. Khosravi, F., & Fathi-Nasri, M. H. (2012). Effect of drying and ensiling of pomegranate seed pulp on its chemical composition and ruminal degradability parameters. Animal Production, 14 (2), 51-61. (In Persian).
  18. Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate pulp extract in comparison with pomegranate pulp extract. Food Chemistry, 96(2), 254-260. http://dx.doi.org/1016/j.foodchem.2005.02.033
  19. Mahapatra, K., Nanda, R., Bag, S., Banerjee, R., Pandey, A., & Szakacs, G. (2005). Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori Nakazawa. Processes in Biochemistry, 40 (10), 3251-3254.
  20. Makkar, H. P. S., Singh, B., & Negi, S. S. (1989). Relationship of rumen degradability with biomass accumulation, cell wall constituents and tannin levels in some tree leaves. Animal Production, 49(2), 299-303.
  21. Modaresi S. J., Valizadeh, R., Danesh-Mesgaran, M., & Fathi-Nasri, M. H. (2015). Effect of feeding pomegranate seed pulp silageon blood metabolites, carcass characteristics and performance of kids. Journal of Ruminant Research, 3(2): 77-91. (In Persian).
  22. Mousa, M. R. M. (2011). Effect of feeding Acacia as supplements on the nutrient digestion, growth performance, carcass traits and some blood constituents of Awassi lambs under the conditions of North Sinai. Asian Journal of Animal Sciences, 5(2), 102-117. http://dx.doi.org/3923/ajas.2011.102117
  23. Mueller-Harvey, I. (2006). Unravelling the conundrum of tannins in animal nutrition and health. Journal of Science of Food and Agriculture, 86 (13), 2010-2037. http://dx.doi.org/1002/jsfa.2577
  24. Raghuwanshi, S., Misra, S., & Saxena, R. K. (2014). Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants. Journal of Animal Science and Biotechnology, 5(1), 1-8. http://dx.doi.org/1186/2049-1891-5-13
  25. Sadq, S. M., Ramzi, D. O. M., Hamasalim, H. J., & Ahmed, K. A. (2016). Growth performance and digestibility in Karadi lambs receiving different levels of pomegranate pulps. Open Journal of Animal Sciences, 6(01), 16. http://dx.doi.org/4236/ojas.2016.61003
  26. Sallam, S. M. A., Attia, M. F. A., Nour El-Din, A. N. M., El-Zarkouny, S. Z., Saber, A. M., El-Zaiat, H. M., & Zeitoun, M. M.  (2019). Involvement of Quebracho tannins in diet alters productive and reproductive efficiency of postpartum buffalo cows. Animal Nutrition, 5(1), 80-86.  http://dx.doi.org/1016/j.aninu.2018.08.003
  27. Sreelatha, S. & Padma, P. (2009). Antioxidant activity and total phenolic con-tent of Moringa oleifera leaves in two stages of maturity. Plant Food Human Nutrition, 64(4), 303-311. http://dx.doi.org/1007/s11130-009-0141-0
  28. Shakeri, P., Riasi, A., Alikhani, M., Fazaeli, H., & Ghorbani, G. R. (2012). Effects of feeding pistachio by-products silage on growth performance, serum metabolites and urine characteristics in Holstein male calves. Journal of Animal Physiology and Animal Nutrition, 97(6), 1022–1029. http://dx.doi.org/1111/jpn.12005
  29. Shabtay, A., Harel, A., Yaakov, T., Alla, O., Ayala, M., Pnina, W., Zwika, G., Yaira, C., Arieh, B., Ido, I., & Zohar, K. (2008). Nutritive and anti-oxidative potential of fresh and stored pomegranate industrial byproduct as novel beef cattle feed. Journal of Agricultural and Food Chemistry, 56(21), 10063-10070. http://dx.doi.org/1021/jf8016095
  30. Shabtay, A., Nikbachat, M., Zenou, A., Yosef, E., Arkin, O., Sneer, O., & Miron, J.. (2012). Effects of adding a concentrated pomegranate extract to the ration of lactating cows on performance and udder health parameters. Animal Feed Science and Technology, 175(1-2), 24-32. http://dx.doi.org/22059/jap.2012.32042
  31. Szczechowiak, J., Szkudelska, K., Szumacher-Strabel, M., Sadkowski, S., Gwozdz, K., El-Sherbiny Kzlowska, M., Rodriguez, V., & Cieslak, A. (2017). Blood hormones, metabolic parameters and fatty acid proportion in dairy cows fed condensed tannins and oils blend. Annals of Animal Science, 18(1), 155-166. http://dx.doi.org/1515/aoas-2017-0039.
  32. Van Keulen, J., & Young, B. A. (1977). Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44(1): 282–287. http://doi.org/2527/jas 1997.442282x
  33. Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal’s nutrition. Journal of Dairy Science, 74: 3583-3592. http://doi.org/10.3168/jds.S0022- 0302(91)78551-2
  34. Walum, E. (1998). Artery stenosis (CAS) reduces common carotid intima-media thickness (IMT), blood pressure and LDL oxidation, Environmental Health Perspectives. Clinical Nutrition, 23(1), 423-433.
  35. Yanez-Ruiz, D. R., Scollan, N. D., Merry, R. J., & Newbold, C. J. (2006). Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their soluble carbohydrate content. British Journal of Nutrition. 96(5), 861-869. http://dx.doi.org/1017/bjn20061927
  36. Zare, A. (2015). The effect of feeding silage pomegranate pulp (mixture of peel and seeds) and dried pomegranate seed pulp on the fattening performance of Mehrban lambs. M.Sc. Thesis, Shiraz University, Iran. (In Persian).
  37. Zurek, E., Foxcroft, G. R., & Kennelly, J. J. (1995). Metabolic status and interval to first ovulation in postpartum dairy cows. Journal of Dairy Science, 78(9), 1909-1920. http://dx.doi.org/3168/jds.S0022-0302(95)76816-3
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