Therapeutic intervention for the treatment of Caseous lymphadenitis using intra-abscess instillation of Ozone or hydrogen peroxide in small ruminants

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 مرداد 1402   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2023.82086.1246

نویسندگان

Gholam Ali Khorshidian¹؛ Ramin Moradi¹؛ Omid Rajabi²؛ Behnaz Norouzi³؛ Mahdi Askari Badouei⁴؛ Kamran Sharifi^{* 1}

¹Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad , Iran

²Department of Pharmaceutical control, school of pharmacy, Mashhad university of medical sciences (mums), Iran

³Dr. Norouzi Private veterinary Practice, Hadji Abad, South Khorasan province, Iran

⁴Department of Pathobiology, school of Veterinary Medicine, Ferdowsi University of Mashhad, Iran

چکیده

Background- Caseous lymphadenitis (CLA) is an economically and zoonotically important disease in the world. The lack of a therapeutic procedure limits the treatment mainly to surgical intervention.Objectives- Therapeutic efficacy of intra-abscess instillation of olive oil containing Ozone (O3-oil) and glycerin-containing hydrogen peroxide (H2O2-gly) in CLA in small ruminants was tested.Methods- 180 affected sheep and goats were allocated to 5 groups as follows: 1) negative control (no intervention-NC), 2) positive control group 1 (injection of olive oil, PC1), 3) positive control group 2 (injection of glycerin), 4) injection of O3-oil, 5) injection of H2O2-gly. Samples of abscess contents were gathered for microbiological examination prior to injection, as well. Volumes of abscesses (VAs) were measured initially (T0), then with two-week intervals at Time 1 (T1) and Time 2 (T2).Results- At T0 and T2 VAs were as follows, respectively: NC (2.9±0.5; 3.5±0.5), PC1(3.4±0.7; 6.6±1), PC2 (3.1±0.7; 3.3±0.9), O3-oil (3.3±0.4; 0.4±0.4), and H2O2-gly (4.6±0.4, 1.5±0.4), showing statistically significant decreases in VAs, merely in treatment groups. Corynebacterium pseudotuberculosis was recovered in 48.3% of bacteriological samples.Conclusions- The results suggest that O3-oil and H2O2-gly would be reliable therapeutic agents in treating and control of CLA. Ozone showed higher efficacy and more rapid shrinkage/recession of the abscesses.

کلیدواژه‌ها

Abscess؛ Caseous lymphadenitis؛ Ozone؛ Hydrogen Peroxide؛ Small Ruminants؛ Treatment