The antimicrobial activity of peppermint (Mentha piperita) and pennyroyal (Mentha pulegium) essential oil on three mastitis-causing pathogens in milk

Iranian Journal of Veterinary Science and Technology

دوره 15، شماره 4 - شماره پیاپی 33، اسفند 2023، صفحه 29-36   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2023.80895.1227

نویسندگان

Reza Rahchamani^{* 1}؛ Javad Bayat Kouhsar²؛ Samira Noori¹

¹Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran.

²Graduated from Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran.

چکیده

Bovine mastitis causes a lot of economic losses, and the appearance of resistant strains of bacteria has led to the use of alternative natural bioagents for treatment. It is generally believed that high levels of fat and/or protein in foods may protect bacteria against the effects of essential oils (EOs). The purpose of this paper was to investigate the effect of EOs of Mentha piperita (peppermint) and Mentha pulegium (pennyroyal) on three bovine mastitis bacteria (Escherichia coli, Streptococcus agalactiae, and Staphylococcus aureus) in milk. Gas chromatography/mass spectrometry was used for the analysis of EOs. Antibacterial effects of the EOs on bacteria were evaluated with minimum bactericide concentration (MBC), minimum inhibitory concentration (MIC), and time-kill assay. Major components of peppermint EO were carvone (63.02%) and limonene (24.48%), and those of pennyroyal EO were pulegone (48.16%), eucalyptol (14.57%), and piperitenone (10.09%). The MIC and MBC were 0.62% and 1.25% for pennyroyal, 0.31-1.25% and 0.62-2.5% for peppermint, 0.31-0.62% and 0.62-2.5% for peppermint and pennyroyal, respectively. At 6-h, the bacterial reduction of treatments compared to the control group was significant for E. coli and S. agalactiae bacteria. The S. agalactiae and S. aureus counts significantly decreased in the peppermint and pennyroyal group at 24-h. In conclusion, peppermint and pennyroyal EO showed an antibacterial effect on these three bacteria and can be evaluated as an adjunct or alternative to antibiotics in the treatment of bovine mastitis.

کلیدواژه‌ها

Antibacterial effect؛ mastitis؛ Mentha piperita؛ Mentha pulegium؛ milk

سایر فایل های مرتبط با مقاله

4-1227- persian Abs-corrected-MT-F.pdf

مراجع

1.     Montironi ID, Cariddi LN, Reinoso EB. Evaluation of the antimicrobial efficacy of Minthostachys verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis. Rev Argent Microbiol. 2016;48(3):210–6. doi:10.1016/j.ram.2016.04.005.
2.     Alekish MO, Ismail ZB, Awawdeh MS, Shatnawi S. Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis. Vet World. 2017;10(8):895–900. doi:10.14202/vetworld.2017.895-900.
3.     Perini S, Piccoli RH, Nunes CA, Bruhn FRP, Custódio DAC, Costa GM. Antimicrobial activity of essential oils against pathogens isolated from Bovine Mastitis. J Nat Prod Plant Resour. 2014;4(2):6–15.
4.    Ananda Baskaran S, Kazmer GW, Hinckley L, Andrew SM, Venkitanarayanan K. Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogens in vitro. J Dairy Sci. 2009; 92(4): 1423–1429. 
5.     Desam NR, Al-Rajab AJ, Sharma M, Mylabathula MM, Gowkanapalli RR, Albratty M. Chemical constituents, in vitro antibacterial and antifungal activity of Mentha × Piperita L. (peppermint) essential oils. J King Saud Univ. 2019;31(4):528–33. doi:10.1016/j.jksus.2017.07.013.
6.     Saharkhiz MJ, Motamedi M, Zomorodian K, Pakshir K, Miri R, Hemyari K. Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil of Mentha piperita L. . ISRN Pharm. 2012; 2012:1–6. doi:10.5402/2012/718645.
7.     Parham S, Kharazi AZ, Bakhsheshi-Rad HR, Nur H, Ismail AF, Sharif S, et al. Antioxidant, antimicrobial and antiviral properties of herbal materials. Antioxidants. 2020;9(12):1–36. doi:10.3390/antiox9121309.
8.     Contreras GA, Rodríguez JM. Mastitis: Comparative etiology and epidemiology. J Mammary Gland Biol Neoplasia. 2011;16(4):339–56. doi:10.1007/s10911-011-9234-0.
9.     Zhu H, Du M, Fox L, Zhu MJ. Bactericidal effects of Cinnamon cassia oil against bovine mastitis bacterial pathogens. Food Control. 2016;66:291–9.doi:10.1016/j.foodcont.2016.02.013.
10.     Noori S, Rahchamani R, Kohsar JB, Binabaj FB. Antibacterial effect of Lavandula stoechas and Origanum majorana essential oils against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. Iran J Vet Sci Technol. 2019;11(1):35-41. doi:10.22067/veterinary.v1i11.77679.
11.     Zarooni S, Rahchamani R, Ghanbari F, Khanahmadi A. Antibacterial effect of Satureja hortensis and Salvia officinalis essential oils against major bovine mastitis bacteria. Iran J Vet Sci Technol. 2021;13(1):75–81.doi:10.22067/ijvst.2021.68752.1017.
12.     Kot B, Wierzchowska K, Piechota M, Czerniewicz P, Chrzanowski G. Antimicrobial activity of five essential oils from lamiaceae against multidrug-resistant Staphylococcus aureus. Nat Prod Res. 2019;33(24):3587–91. doi:10.1080/14786419.2018.1486314.
13.     Sartoratto A, Machado ALM, Delarmelina C, Figueira GM, Duarte MCT, Rehder VLG. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian J Microbiol. 2004; 35(4):275–80. doi:10.1590/S1517-83822004000300001.
14.     Moghaddam M, Pourbaige M, Tabar HK, Farhadi N, Hosseini SMA. Composition and antifungal activity of peppermint (Mentha piperita) essential oil from Iran. J Essent Oil-Bearing Plants. 2013;16(4):506–12. doi:10.1080/0972060X.2013.813265.
15.     Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry. 2006;67(12):1249–55. doi:10.1016/j.phytochem.2006.04.025.
16.    Aggarwal KK, Khanuja SPS, Ateeque A, Santha kumar TR, Vivek KG, Kumar S. Antimicrobial Activity Profiles of the Two Enantiomers of Limonene and Carvone Isolated from the Oils of Mentha Spicata and Anethum Sowa. Flavour and Fragrance J. 2002; (17): 59–63. doi:10.1002/ffj.1040.
17.    Lešnik S, Furlan V,  Bren U. Rosemary (Rosmarinus Officinalis L.): Extraction Techniques, Analytical Methods and Health-Promoting Biological Effects. Phytochemistry Rev. 2021; 20 (6): 1273-1328. doi:10.1007/s11101-021-09745-5.
18.     Dehghani N, Afsharmanesh M, Salarmoini M, Ebrahimnejad H. Characterization of pennyroyal (Mentha pulegium) essential oil as an herbal, antibacterial, and antioxidant substance. Comp Clin Path. 2018;27(6):1575–81. doi:10.1007/s00580-018-2776-4.
19.     Luís Â, Domingues F. Screening of the potential bioactivities of pennyroyal (Mentha pulegium L.) essential oil. Antibiotics. 2021;10(10):1266. doi:10.3390/antibiotics10101266.
20.     Teixeira B, Marques A, Ramos C, Batista I, Serrano C, Matos O, et al. European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Ind Crops Prod. 2012;36(1):81–7. doi:10.1016/j.indcrop.2011.08.011.
21.     Kang J, Jin W, Wang J, Sun Y, Wu X, Liu L. Antibacterial and anti-biofilm activities of peppermint essential oil against Staphylococcus aureus. Lwt- Food Sci Technol. 2019;101:639–45. doi:10.1016/j.lwt.2018.11.093.
22.     Catherine AA, Deepika H, Negi PS. Antibacterial activity of eugenol and peppermint oil in model food systems. J Essent Oil Res. 2012;24(5):481–6. doi:10.1080/10412905.2012.703513.
 23.    Mahboubi M, Haghi G. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. J Ethnopharmacol. 2008; 119: 325–7. doi: 10.1016/j.jep.2008.07.023.
24.    Rahchamani R, Noori S, Bayat Kouhsar J. Antibacterial effect of essential oils of peppermint and pennyroyal on major bovine mastitis bacteria. New Find Vet Microbio. 2023; 6(1): 64-75. doi:10.22034/NFVM.2023.385595.1175.
25.     Bouyahya A, Et-Touys A, Abrini J, Talbaoui A, Fellah H, Bakri Y, et al. Lavandula stoechas essential oil from Morocco as novel source of antileishmanial, antibacterial and antioxidant activities. Biocatal Agricul Biotechnol. 2017; 12: 179-184. doi:10.1016/j.bcab.2017.10.003.
26.    Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol. 2004; 94:223–53. doi:10.1016/j.ijfoodmicro.2004.03.022.
27.     Gaysinsky  sylvia, Taylor TM, Davidson PM, Bruce BD, Weiss J. Antimicrobial efficacy of eugenol microemulsions in milk against Listeria monocytogenes and Escherichia coli O157:H7. J Food Prot. 2007;70(11):2631–7. doi:10.4315/0362-028X-70.11.2631.
28.     Basti AA, Misaghi A, Khaschabi D. Growth response and modelling of the effects of Zataria multiflora Boiss. essential oil, pH and temperature on Salmonella Typhimurium and Staphylococcus aureus. LWT - Food Sci Technol. 2007 Aug;40(6):973–81. doi:10.1016/j.lwt.2006.07.007.