Gene silencing method based on DNA

Journal of Cell and Molecular Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 15 آبان 1402

نوع مقاله: Review / Mini-Review

شناسه دیجیتال (DOI): 10.22067/jcmr.2023.84052.1077

نویسندگان

Mitra Riasi¹؛ Elnaz Karbaschian²؛ Ali Javadmanesh^{* 1}

¹Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad

²Department of Animal Science, Faculty of Agriculture, Ferdoswi University of Mashhad

چکیده

DNA-based approaches can now be utilized as low-risk methods to change gene expression. It appears that this approach has the power to partially replace RNA-based approaches for altering gene expression, which in the majority of cases lead to immunological responses in patients. When utilized as a technique to silence target gene expression, DNA interference (DNAi) is a single-stranded DNA created to complement the upstream region of a gene. This DNAi molecule is stabilized using a variety of chemical changes, including phosphorothioates, methylphosphonatesetc, etc. Several studies of the effective application of DNA-based methods in eukaryotic cell lines and in the therapy of various disorders, such as Duchenne muscular dystrophy, cancer, etc., have been mentioned. Understanding the DNAi process, its transfer carriers, stabilization techniques, and their limitations is crucial for advancing these applications and predicting the future of DNAi in both basic science and the treatment of disorders brought on by abnormal gene expression. The objective of this review is mainly introducing benefits of using DNAi in gene silencing. In this review, different applications of DNAi in drug discovery and treatment, criteria of designing DNAi, possible modifications, introducing different types of carriers and limitations of DNAi administration have been discussed. Understanding the DNAi process, its transfer carriers, stabilization techniques, and their limitations is crucial for advancing these applications and predicting the future of DNAi in both basic science and the treatment of disorders brought on by abnormal gene expression. The objective of this review is mainly introducing benefits of using DNAi in gene silencing. In this review, different applications of DNAi in drug discovery and treatment, criteria of designing DNAi, possible modifications, introducing different types of carriers and limitations of DNAi administration have been discussed.

کلیدواژه‌ها

DNAi؛ gene silencing؛ phosphorothioates؛ gene therapy