تجزیه و تحلیل اثرات تنظیمی lncRNAها بر بافت کلیه در جوجه‌های گوشتی مبتلا به سندروم آسیت

پژوهشهای علوم دامی ایران

مقاله 6، دوره 16، شماره 2 - شماره پیاپی 58، تیر 1403، صفحه 235-247   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ijasr.2023.83528.1162

نویسندگان

کسری احمدیان¹؛ محمدرضا نصیری^{* 2}؛ کریم حسن پور³؛ علی جوادمنش⁴

¹گروه علوم دامی ، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد ، ایران

²گروه علوم دامی ، دانشکده کشاورزی ، دانشگاه فردوسی مشهد، مشهد ، ایران

³گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز ، ایران

⁴گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران.

چکیده

آسیت یک اختلال متابولیکی مهم در صنعت طیور تجاری می­باشد که بافت­های متفاوتی از جمله کلیه را درگیر می­کند. در این پژوهش، ژن­های غیررمزکننده بلند(lncRNA) مؤثر در بیماری آسیت در جوجه‌های گوشتی که اثر تنظیمی روی ژن‌های mRNA هدف دارند با استفاده از فناوری RNA-seq مورد بررسی قرار گرفت. در روز 39 آزمایش، از بافت کلیه، چهار نمونه سالم و چهار نمونه بیمار RNAکل استخراج و با استفاده از پلتفرم illumina Hiseq2500 توالی‌یابی انجام شد. بر اساس نتایج آنالیز RNA-seq، تعداد 1421 رونوشت lncRNA از 921 جایگاه ژنی شناسایی شد که با 154 ژن رمزشونده در محدوده 100 هزار باز بالادست و پایین‌دست ژن‌‌های مورد نظر ارتباط داشتند. از میان جفت ژن‌‌های رمزشونده و lncRNA، آن‌هایی که همبستگی بیانی بیشتر از مثبت 90 درصد و کمتر از منفی 90 درصد داشتند، به‌ترتیب به‌عنوان جفت ژن‌‌‌‌های با بیان هم‌راستا و غیر هم‌راستا مورد ارزیابی قرار گرفتند. بررسی عملکردی این ژن­ها نشان داد که 13 ژن به‌طور معنی­داری در مسیرهای متعدد زیستی مرتبط با بیماری مشاهده شدند. با هدف قراردادن مسیرهای مهم زیستی به‌دست آمده و ژن­های مؤثر بر آن­ها شامل C1GALT1C1، PLCD4، GCNT4، DGKA، IMPA2، GBA2، CERS5، CDH1، CSF1، CTNND1، LPAR2، DAO، PIPOX و تمرکز بر نقش آن‌ها در پیشگیری و درمان بیماری آسیت، درک بهتری از عملکرد آن‌ها را در تأمین انرژی و حفظ هموستاز کلیه، ممکن ساخته تا جهت پیشگیری و درمان بیماری آسیت، بینشی جدید برای اصلاح نژاد و کاهش مشکلات مربوط به این بیماری در گله‌های تجاری ارائه دهد.
 
 
 
 
 
 

کلیدواژه‌ها

آسیت؛ جوجه گوشتی؛ کلیه؛ lncRNA؛ RNA-seq

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