Effects of Caffeine Ingestions Concomitant to Incremental Running Test on Lipid Peroxidation and Enzymatic Antioxidant in Active Men

Research in Exercise and Sports Medicine

دوره 5، شماره 1، شهریور 2014، صفحه 9-15   

نوع مقاله: Original Article

چکیده

Purpose: The aim of the current research was to assess effects of caffeine (5 and 9 mg.kg -1 ) ingestions 
concomitant to incremental running test on malondialdehyde (MDA) as lipid peroxidation marker and 
superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) as enzymatic antioxidants. 
Material and Methods: Ten subjects were tested on a treadmill until exhaustion on three separate occasions 
between which there were intervals of 5 days. Every volunteer underwent three conditions containing placebo 
or caffeine doses that were consumed one hour before exercise (pre-test). Blood samples were collected pretest, before caffeine or placebo consumption and immediately after exercise (post-test). 
Results: Results showed that in comparison to placebo condition, caffeine doses decreased MDA levels and 
increased GPx activities significantly (P< 0/05). Regardless of 5 mg.kg -1 caffeine ingestion that had no 
significant effect on CAT and SOD activities (P< 0/05), 9 mg.kg -1 caffeine intake significantly increased and 
decreased CAT and SOD respectively (P< 0/05), when compared to placebo. For MDA levels, there was no 
significant difference between caffeine doses (P< 0/05), but these differences for CAT, SOD and GPx were 
significant (P< 0/05). 
Discussion and Conclusion: In conclusion caffeine doses approximately had equal effects on lipid 
peroxidation decline, while these effects on enzymatic antioxidant with 9 mg.kg -1 caffeine intake were better 
than 5 mg.kg-1 caffeine

کلیدواژه‌ها

Oxidative stress؛ Free radicals؛ Progressive exercise؛ Bruce protocol؛ Caffeine doses