In silico identification of a putative lncRNA-miRNA regulatory network associated with mucin regulation in colon cancer

Journal of Cell and Molecular Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 25 بهمن 1402

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2024.86231.1086

نویسندگان

Sayyed Emad Aldin Tayyebi¹؛ Mahyar Heydarpour²؛ Hesam Dehghani^{* 3}

¹Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad

²Department of Medicine, Division of Endocrinology, Brigham & Women's Hospital, Harvard Medical School, Boston 02115, MA, USA

³Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

Colorectal cancer is one of the most common cancers and is one of the leading causes of cancer-related deaths worldwide. The underlying biological mechanisms for the development of colorectal cancer are largely unidentified. Several genes have been identified that are most likely involved in the pathogenesis of colorectal cancer disease. However, some other genes might have less evident functions. One gene family with prominent functional roles in the normal colon is mucin. Multiple studies have demonstrated the involvement of mucins in the pathogenesis of human malignancies. Therefore, due to the lack of an inclusive investigation for mucins' expression, mechanism of action, and their involvement in the underlying biology, diagnosis, and prognosis of colon adenocarcinoma, we sought to unearth their potential involvement and their related regulatory networks in this disease. In this investigation, through a stepwise manner, a plethora of databases and algorithmic tools were applied. Due to a significant upregulation at both mRNA and protein levels and following a thorough evaluation of diagnostic and prognostic values in colon adenocarcinoma, MUC13 was determined to be the most relevant regulatory mucin in colon carcinoma. Altogether, these findings indicate a putative ncRNA-mRNA network, including hsa-mir-136-5p, hsa-mir-27a-3p, NEAT1, and XIST to be involved in regulating MUC13 in colon cancer. This stepwise investigation implies that MUC13 may have a crucial role in the underlying molecular mechanisms for the initiation or progression of colon cancer. In addition, it provides insights into molecular mechanisms and possible regulatory non-coding RNA networks that might be responsible for regulating MUC13 expression.

کلیدواژه‌ها

colon cancer؛ lncRNAs؛ miRNAs؛ mucins؛ MUC13؛ regulatory networks