The Effect of Phenological Stages of Salvia abrotanoides (Kar.) Sytsma on Antibacterial and Antioxidant Potential, Total Phenol and Flavonoid Content of Roots and Aerial Parts

Journal of Cell and Molecular Research

مقاله 5، دوره 15، شماره 2 - شماره پیاپی 30، تیر 2024، صفحه 77-88   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2024.85578.1081

نویسندگان

Arehzoo Zaker^* ؛ Hamed Norouzi Taheri

Department of Biology Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran

چکیده

This study aimed to evaluate the antibacterial and antioxidant activities of methanol and ethyl acetate extracts of 
the roots and aerial parts of Salvia abrotanoides obtained at different phenological stages (vegetative, flowering, and 
seeding) and to determine their total phenol and flavonoid content. Disc diffusion and micro-dilution methods evaluated 
antibacterial activity against eight bacterial strains. Folin-Ciocalteu and aluminum chloride colorimetric methods were 
used to determine the content of total phenol and flavonoids, respectively. The antioxidant potential of the extracts was 
measured using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Staphylococcus aureus and Pseudomonas aeruginosa
were the most sensitive and resistant bacteria to the extracts, respectively. The strongest antibacterial activity against 
multi-drug resistant bacteria was recorded for methicillin-resistant Staphylococcus aureus treated with ethyl acetate 
extract of the root at the seeding stage, in which MIC and MBC values were 30.33 and 40.00 mg/mL, respectively. The 
highest content of total phenol (557.51 mg GAE/g DW) and flavonoids (236.40 mg QE/g DW) was found in the ethyl 
acetate extract of the aerial parts in the seeding phase. The aerial parts had more total phenolic and flavonoid content at 
different phenological stages than the root. The antioxidant capacity of the aerial part was also better that of the roots. 
The ethyl acetate extract of the aerial part at the seeding phase presented the highest DPPH scavenging activity (92.51 ± 
1.25 %). The results showed that S. abrotanoides extracts, especially at the seeding phase, have good potential as a source of antioxidant, antibacterial, and bioactive compounds and can be considered good candidates in the development of new drugs or as the main source of food preservative compounds.

کلیدواژه‌ها

Antibacterial؛ Bio compounds؛ Drug-resistant bacteria؛ MRSA؛ Phenology؛ Salvia