Helicobacter Detection in the Stomach of Apparently Normal Donkeys: Sampling, Diagnostic Methods, and Implications for Equine Glandular Gastric Disease and Serum Antioxidant Status

Iranian Journal of Veterinary Science and Technology

دوره 16، شماره 2 - شماره پیاپی 35، شهریور 2024، صفحه 50-56   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2024.87403.1366

نویسندگان

Masoud Ahmadnejad¹؛ Ghader Jalilzadeh-Amin^{* 2}؛ Monireh Khordadmehr³

¹Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.

²Department of Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

³Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

چکیده

The involvement of Helicobacter-like agents in EGGD is not clear. Much evidence supports the presence of Helicobacter in the horse’s stomach, but it is not so clear about the donkey. The present study was conducted to evaluate the presence of Helicobacter in the stomach of donkeys and assess the best method for its identification and its possible participation in EGGD and serum antioxidant status. Gastric juice sampling and biopsy from NMP and PA were performed by gastroscopic method from 12 donkeys. Histopathology and RUT were used to identify Helicobacter. TAC, THIOL, NO, and DPPH were measured to assess the antioxidant status of serum. Helicobacter was detected only by the RUT method in one donkey (8.33%), but it is unlikely that this infection was effective in causing EGGD. The RUT results for the three samples of gastric juice, NMP, and PA were not different. Histopathology of NMP and PA did not show Helicobacter infection. Helicobacter is present in the stomach of a donkey but does not change the antioxidant status of serum. The RUT is more efficient than H&E histopathology for the assessment of H. pylori in the equine stomach, and the RUT of gastric juice is preferable to tissue samples because it is easy, fast, and non-invasive. In conclusion, further studies using more accurate methods are recommended to evaluate the effect of Helicobacter in the gastric diseases of donkeys and other equine.
 

کلیدواژه‌ها

Equine Gastric Ulcer Syndrome؛ Donkey؛ Microbiome؛ Antioxidant

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مراجع

1. Sykes BWW, Hewetson M, Hepburn RJJ, Luthersson N, Tamzali Y. European College of Equine Internal Medicine Consensus Statement-Equine Gastric Ulcer Syndrome in Adult Horses. J Vet Intern Med. 2015;29:1288–99. Doi:10.1111/jvim.13578.
2. Voss SJ, McGuinness DH, Weir W, Sutton DGM. A study comparing the healthy and diseased equine glandular gastric microbiota sampled with sheathed transendoscopic cytology brushes. J Equine Vet Sci. 2022;116:104002. Doi:10.1016/J.JEVS.2022.104002.
3. Paul LJ, Ericsson AC, Andrews FM, Keowen ML, Morales Yniguez F, Garza F, Banse HE. Gastric microbiome in horses with and without equine glandular gastric disease. J Vet Intern Med. 2021;35:2458–64. Doi:10.1111/JVIM.16241.
4. Rendle D, Bowen M, Brazil T, Conwell R, Hallowell G, Hepburn R, Hewetson M, Sykes B. Recommendations for the management of equine glandular gastric disease. UK-Vet Equine. 2018;2:2–11. Doi:10.12968/ukve.2018.2.s1.3.
5. Hewetson M. Managing glandular gastric disease in horses. In Pract. 2021;43:217–28. Doi:10.1002/INPR.40.
6. Husted L, Jensen TK, Olsen SN, Mølbak L. Examination of equine glandular stomach lesions for bacteria, including Helicobacter spp by fluorescence in situ hybridisation. BMC Microbiol. 2010;10:1–8. Doi:10.1186/1471-2180-10-84/FIGURES/6.
7. Graham DY. History of Helicobacter pylori, duodenal ulcer, gastric ulcer and gastric cancer. World J Gastroenterol. 2014;20:5191. Doi:10.3748/WJG.V20.I18.5191.
8. Simpson K, Neiger R, DeNovo R, Sherding R. The relationship of Helicobacter spp. infection to gastric disease in dogs and cats. J Vet Intern Med. 2000;14:223–7. Doi:10.1111/j.1939-1676.2000.tb02243.x.
9. Sykes B, Jokisalo JM. Rethinking equine gastric ulcer syndrome: Part 3 – Equine glandular gastric ulcer syndrome (EGGUS). Equine Vet Educ. 2015;27:372–5. Doi:10.1111/EVE.12287.
10. Hepburn RJ, Mrcvs B, Furr Dvm MO. Investigation into the Presence of Helicobacter in the Equine Stomach by Urease Testing and Polymerase Chain Reaction and Further Investigation into the Application of the 13C-Urea Blood Test to the Horse. 2004.
11. Moyaert H, Decostere A, Pasmans F, Baele M, Ceelen L, Smits K, Ducatelle R, Haesebrouck F. Acute in vivo interactions of Helicobacter equorum with its equine host. Equine Vet J. 2007;39:370–2. Doi:10.2746/042516407X206391.
12. Fonseca CB, Braga L, Silva Costa Ferreira C, Emerin Pinna A, Silva Leite J, Ferreira AM. Immunohistochemical Identification of Helicobacter Spp and Evaluation of the Degree of Inflammation in the Region of the Margo Plicatus of the Equine Stomach. SSRN Electron J. 2022. Doi:10.2139/ssrn.3983892.
13. Rezazadeh F, Pourebrahimi N, Ghotaslou R, Nasab MG, Memar MY. Frequency of endoscopic findings of equine squamous gastric disease (ESGD) in rural horses and its association with Helicobacter pylori virulence genes. Vet Med (Praha). 2022;67:61–9. Doi:10.17221/154/2020-VETMED.
14. Moyaert H, Decostere A, Vandamme P, Debruyne L, Mast J, Baele M, Ceelen L, Ducatelle R, Haesebrouck F. Helicobacter equorum sp. nov., a urease-negative Helicobacter species isolated from horse faeces. Int J Syst Evol Microbiol. 2007;57:213–8. Doi:10.1099/IJS.0.64279-0/CITE/REFWORKS.
15. Dong HJ, Ho H, Hwang H, Kim Y, Han J, Lee I, Cho S. Diversity of the Gastric Microbiota in Thoroughbred Racehorses Having Gastric Ulcer. J Microbiol Biotechnol. 2016;26:763–74. Doi:10.4014/JMB.1507.07054.
16. Contreras M, Morales A, García-Amado MA, De Vera M, Bermúdez V, Gueneau P. Detection of Helicobacter-like DNA in the gastric mucosa of Thoroughbred horses. Lett Appl Microbiol.2007;45:553–7. Doi:10.1111/j.1472-765X.2007.02227.x.
17. Fox JG. The non-H pylori helicobacters: Their expanding role in gastrointestinal and systemic diseases. Gut. 2002;50:273–83. Doi:10.1136/gut.50.2.273.
18. Abelardo MB, Garcia F, Bermudez V. Detection of helicobacter-like organisms in Thoroughbred horses from Venezuela. Brazilian J Vet Pathol. 2010;3:52–5.
19. Sprayberry KA, Robinson NE. Robinson’s Current Therapy in Equine Medicine: Seventh Edition. 2014. Doi:10.1016/C2011-0-05761-7.
20. Morales A, Lamprea Garrido A, Méndez Sánchez A. Gastric Ulcers Syndrome in Donkeys. Rev Med Vet (Bogota). 2015:31–5.
21. Rezazadeh F, Gharehaghajlou Y. Endoscopic Finding of Gastric Ulcer in Rural Horse and Relation with Gasterophilus spp. Iran J Vet Med. 2020;14:45–52. Doi:10.22059/ijvm.2019.277457.1004975.
22. Baykan AR, Biçer C, Gerçeker E, Erel Ö, Cerrah S, Albayrak B, Utlu M, Kargılı A. Thiol/disulphide homeostasis in Helicobacter pylori infected patients. Eur Res J. 2019. Doi:10.18621/eurj.443557.
23. Zuluaga-Cabrera AM, Jaramillo-Morales C, Martínez-Aranzales JR, Zuluaga Cabrera AM, Jaramillo Morales C, Martínez Aranzales JR. Presence of Helicobacter spp. in dental tartar and gastric mucosa, and its relationship with EGUS in horses from a public slaughterhouse. Rev Colomb Ciencias Pecu. 2022;35:109–17. Doi:10.17533/udea.rccp.v35n1a06.
24. Bezdekova B, Futas J. Helicobacter species and gastric ulceration in horses: a clinical study. Vet Med (Praha). 2009;54:577–82.
25. Fernandes KA, Rogers CW, Gee EK, Kittelmann S, Bolwell CF, Bermingham EN, Biggs PJ, Thomas DG. Resilience of Faecal Microbiota in Stabled Thoroughbred Horses Following Abrupt Dietary Transition between Freshly Cut Pasture and Three Forage-Based Diets. Anim  an Open Access J. from MDPI 2021;11. Doi:10.3390/ANI11092611.
26. Perkins GA, den Bakker HC, Burton AJ, Erb HN, McDonough SP, McDonough PL, Parker J, Rosenthal RL, Wiedmann M, Dowd SE, Simpson KW. Equine stomachs harbor an abundant and diverse mucosal microbiota. Appl Environ Microbiol. 2012;78:2522–32. Doi:10.1128/AEM.06252-11.
27. Liu G, Bou G, Su S, Xing J, Qu H, Zhang X, Wang X, Zhao Y, Dugarjaviin M. Microbial diversity within the digestive tract contents of Dezhou donkeys. PLoS One. 2019;14:e0226186. Doi:10.1371/journal.pone.0226186.
28. Xing J, Liu G, Zhang X, Bai D, Yu J, Li L, Wang X, Su S, Zhao Y, Bou G, Dugarjaviin M. The Composition and Predictive Function of the Fecal Microbiota Differ Between Young and Adult Donkeys. Front Microbiol. 2020;11:3016. Doi:10.3389/FMICB.2020.596394/BIBTEX.
29. Liu X, Fan H, Ding X, Hong Z, Nei Y, Liu Z, Li G, Guo H. Analysis of the gut microbiota by high-throughput sequencing of the v5-v6 regions of the 16s rRNA gene in donkey. Curr Microbiol. 2014;68:657–62. Doi:10.1007/s00284-014-0528-5.
30. Liu H, Zhao X, Han X, Xu S, Zhao L, Hu L, Xu T, Zhao N, Zhang X, Chen D, He F, Chen X. Comparative study of gut microbiota in Tibetan wild asses (Equus kiang) and domestic donkeys (Equus asinus) on the Qinghai-Tibet plateau. PeerJ. 2020;2020:e9032. Doi:10.7717/PEERJ.9032/SUPP-18.
31. Dechant FX, Dechant R, Kandulski A, Selgrad M, Weber F, Reischl U, Wilczek W, Mueller M, Weigand K. Accuracy of Different Rapid Urease Tests in Comparison with Histopathology in Patients with Endoscopic Signs of Gastritis. Digestion. 2020;101:184–90. Doi:10.1159/000497810.
32. Karthikeyan R, Sundaravadanan BS. Rapid Urease Test vs Histopathological Examination: A Prospective Study to Compare the Accuracy of Both Tests in the Diagnosis of Helicobacter pylori. J Pharm Res Int. 2021:320–5. Doi:10.9734/jpri/2021/v33i49b33369.
33. Noh CK, Lee GH, Park JW, Roh J, Han JH, Lee E, Park B, Lim SG, Shin SJ, Cheong JY, Kim JH, Lee KM. Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa. Sci Rep. 2020;10:1–10. Doi:10.1038/s41598-020-75528-1.
34. Martineau H, Thompson H, Taylor D. Pathology of gastritis and gastric ulceration in the horse. Part 1: Range of lesions present in 21 mature individuals. Equine Vet J. 2009;41:638–44. Doi:10.2746/042516409X464816.
35. Andrews F, Bernard W, Byars N, Cohen T, Divers C, MacAllister A, McGladdery A, Merritt M, Murray, Orsini J, Snyder J, Vatistas N. Recommendations for the diagnosis and treatment of equine gastric ulcer syndrome (EGUS): The Equine Gastric Ulcer Council. Equine Vet Educ. 1999;11:262–72. Doi:10.1111/j.2042-3292.1999.tb00961.x.
36. Pearson RA, Ouassat M. A guide to live weight estimation and body condition scoring of donkeys. University of Edinburgh, Centre for Tropical Veterinary Medicine; 2000.
37. Cecchini S, Fazio F. Assessment of total antioxidant capacity in serum of heathy and stressed hens. Animals. 2020;10:1–8. Doi:10.3390/ani10112019.
38. Lopez-Sanroman F. Endoscopy of the upper airways and the proximal digestive tract in the donkey (Equus asinus). J Equine Vet Sci. 2001;21:17–20. Doi:10.1016/S0737-0806(01)70233-X.
39. Lucas Castillo JA, Gozalo-Marcilla M, Werneck Fonseca M, Possebon FS, da Rosa AC, de Araujo Aguiar AJ. Sedative and cardiorespiratory effects of low doses of xylazine with and without acepromazine in Nordestino donkeys. Equine Vet J. 2018;50:831–5. Doi:10.1111/evj.12835.
40. Ahmadnejad M, jalilzadeh-amin G, Sykes BW. Prophylactic effects of Glycyrrhiza glabra root extract on phenylbutazone-induced Equine Glandular Gastric Disease (EGGD). J Equine Vet Sci. 2022:104088. Doi:10.1016/J.JEVS.2022.104088.
41. Sykes BW, Jokisalo JM. Rethinking equine gastric ulcer syndrome: Part 1 – Terminology, clinical signs and diagnosis. Equine Vet Educ 2014;26:543–7. Doi:10.1111/EVE.12236.