Intra-hippocampal injection of abscisic acid attenuates learning and memory deficits, and changes oxidative stress indices in REM sleep deprived rats

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 13 اردیبهشت 1404   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2025.86932.1355

نویسندگان

Alireza Fekrat¹؛ Mahzad Abbasnejad²؛ Razieh Kooshki^{* 3}؛ Parastoo Nikkhouy⁴؛ Mehdi Abbasnejad²

¹Department of Psychiatry, Noureh hospital, Kerman University of Medical Sciences, Kerman, Iran

²Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

³Lorestan University

⁴Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman

چکیده

This study evaluated if intra hippocampal administration of ABA is able to modulate learning and memory performance and oxidative stress biomarkers activities in cerebral cortex of rats exposed to rapid eye movement (REM) sleep deprivation. Adult male Wistar rats were cannulated in CA1 area of hippocampus. After recovery, the rats were subjected to REM sleep deprivation for 4 days. Then, the groups of REM sleep deprived (SD) rats were treated with ABA (5, 10, and 15 µg) and ABA (10 µg) + bicuculline (Bic), a competitive GABAA receptor antagonist. Memory and learning were evaluated with Morris water maze (MWM) and shuttle box tests. Moreover, alterations in the levels of catalase as antioxidant enzyme, and MDA and H2O2 as oxidants biomarkers were determined in rat brain cortex. REM SD rats indicated noteworthy learning and memory deficits in both MWM and shuttle box tests when compared to control rats. However, intra-CA1 injection of ABA (10 µg) decreased cognitive impairment in REM SD rats. Bic (1 μg/rat) was not able to change ABA (10 µg) effects. In addition, an increase in catalase activity, and decrease in MDA and H2O2 were indicated in the brain cortex of ABA (10 µg) and ABA+ Bic treated groups. Overall, the data showed ABA aptitude to attenuate REM sleep deprivation-induced learning and memory disruption and oxidative damages in rats. Manipulation of GABAA receptor failed to inhibit ABA effects in REM SD rats.

کلیدواژه‌ها

Sleep deprivation؛ Abscisic acid؛ Bicuculline؛ Learning and memory؛ Rat