Karyological Data of Tanacetum polycephalum Schultz-bip. and T. parthenium Schultz-bip. (Asteraceae) Populations

Journal of Cell and Molecular Research

Article 2, Volume 9, Issue 2 - Serial Number 18, January 0, Pages 59-66   

Document Type: Research Articles

DOI: 10.22067/jcmr.v9i2.59568

Abstract

Chromosome numbers in 19 populations of Tanacetum polycephalum and Tanacetum parthenium from natural resources gene bank, that collected from different regions of Iran, were determined for the first time. The samples prepared by using root tips. After pretreatment, fixation, hydrolysis and staining, the microscopic samples prepared by squash method, metaphases were captured using an optical microscope. The best metaphases plates were selected and used for karyotype analyses. In all of populations the basic chromosome number was x=9 and the populations showed two ploidy levels (diploid & tetraploid). The type of the most chromosomes in all of the populations was metacentric (m) and sub-metacentric (sm) and located in 2A and 2B except for T. parthenium (Yazd, Taft) with 5m+3sm+1st karyotype formula and 2C Stebbins classes. In addition, T. polycephalum (Esfahan, Golpayegan) with the highest value of AR and A1 had karyotype heterogeneity, also T. polycephalum (Esfahan, Golpayegan) and T. polycephalum (West-Azerbaijan, Uromeyeh) had the highest value of chromosome length (TL). Detailed karyotype allows us to group the different populations based on Stebbins classes and asymmetry indices.

Keywords

Chromosome numbers; Ploidy levels, Karyotype; Tanacetum polycephalum; Tanacetum parthenium; Microscopic samples; Squash method

References

1.Altınordu F. and Martin E. (2014) New chromosome counts, karyotype analyses and asymmetry indices in some taxa of genus Senecio L. and related genera Tephroseris (Rchb.) Rchb. and Turanecio Hamzaoglu belong to tribe Senecioneae (Asteraceae) from Turkey. Plant Systematics and Evolution 300: 2205–2216.

2.Carr GD., King RM., Powell AM. and Robinson H. (1999) Chromosome number in Compositeae XVIII. American Journal of Botany 86: 1003-1013.

3.Chehregani A., Atri M., Sarmadi J. and Asgari M. (2011) Chromosome number variation in Tanacetum polycephalum Schultz bip. (l.) (Asteraceae) in west of Iran. Caryologia 64(3): 302-308.

4.Chehregani A. and Hajisadeghian S. (2009) New chromosome counts in some species of Asteraceae from Iran. Nordic Journal of Botany 27: 247-250.

5.Chehregani A., Heidarian M. and Jalali F. (2013) Cytological Investigations on Achillea wilhelmsii C. Koch (Asteraceae) in the Western part of Iran. Chromosome Botany 8:13-16.

6.Chehregani A. and Mehanfar N. (2008) New chromosome counts in the tribe Anthemideae (Asteraceae) from Iran). Cytologia 73(2):189–196.

7.Goldblatt P. and Johnson DE. (1979) The family of Asteraceae. Index to plant chromosome numbers. Missouri Botanical Garden, St. Louis. Available in www.tropicos.org.

8.Guerra M. (2008) Chromosome numbers in plant cytotaxonomy: concepts and implications. Cytogenetic and Genome Research 120: 339–350.

9.Heywood VH. and Humphries CJ. (1977) Anthemideae-systematic review. In: Heywood,V.H.R., Harborne, J.B. and Turner, B.L. (eds). The biology and chemistry of the Compositae. Volume II. Academic Press, Great Britain. pp. 851-897.

10.Hulten E. (1968) Flora of Alaska and Neighboring territories. A manual of the vascular plants. Stanford University Press, Stanford, California. 1008 pp.

11.Huziwara Y. (1962) Karyotype analysis in some genera of compositae. VШ Further studies on the chromosomes of Aster. American Journal of Botany 49:116-119.

12.Inceer H. and Hayirlioglu-Ayaz S. (2007) Chromosome numbers in the tribe Anthemideae (Asteraceae) from north-east Anatolia. Botanical Journal of Linnaean Society 153(2): 203–211.

13.Javadi H., Alizadeh MA., Emami H. and Salehi Shanjani P. (2013) Cytogenetics investigation in different populations of Anthemis tinctoria L. (yellow chamomile) accessions from Iran. Chromosome Science 16: 43-49.

14.Levan A., Fredge K. and Sanberge A.A. (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201-220.

15.Louzada RB., Palma-Silva‌‌‌‌ C., Corrêa AM., Kaltchuk-Santos E. and Wanderley MDGL. (2010) Chromosome number of Orthophytum species (Bromeliaceae). Kew Bull 65: 53-58.

16.Mitich LW. (1992) Intriguing world of weeds-Tansy. Weed Technology 6: 242-244.

17.Mozaffarian V. (2006) A Dictionary of Iranian Plant Names. Latin-English-Persian. Fahang Moaser, 671 pp.

18.Nezhadali A. and Zarrabi-Shirvan B. (2010) Separation, identification and determination of volatile compounds of Ziziphora persica bunge using HS-SPME/GCMS. International Journal of Environmental Science and Development 1(2): 115–118.

19.Peruzzi L., Goralski G., Joachimiak AJ. and Bedini, G. (2012) Does actually mean chromosome number increase with latitude in vascular plants? An answer from the comparison of Italian, Slovak and Polish floras. Comparative Cytogenetics 6: 371–377.

20.Romero Zarco C. (1986) A new method for estimating karyotype asymmetry. Taxon 36: 526-530.

21.Sonboli A., Olanj N. and Pourmirzaei A. (2011) Biosystematics and phylogeny of Tanacetum fisherae, a new record from Iran. Rostaniha (Botanical Journal of Iran) 12 (2): 165-175.

22.Soreng R. J. and Cope E. A. (1991) On the taxonomy of cultivated species of the Chrysanthemum genus-complex (Anthemideae: Compositae). Baileya 23:145-165.

23.Stace CA. (2000) Cytology and cytogenetics as a fundamental resource for 20th and 21th centuries. Taxon 49: 451-477.

24.Stebbins GL. (1971) Chromosomal evolution in higher plants. Edward Arnold Publisher, London, Ltd. p.216.

25.Taner Ö., Gezer E. and Martin E. (2014) Karyotype analyses on the genus Lallemantia fisch. & C. A. Mey. (Lamiaceae) from Turkey. Cytologia 79(4): 553–559.

26.Torrel M., Valles J., Garcia-Jakas N., Mozaffarian V. and Gab-rielian E. (2001) New or rare chromosome counts in the genus Artemisia L. (Asteraceae, Anthemideae) from Armenia and Iran. Botanical Journal of the Linnean Society 135: 51–60.

27.Tutin T., Heywood V., Burges N., Moore D., Valentine D., Walters S. and Webb D. (1976) Flora europaea: plantaginaceae to compositae (and Rubiaceae). Vol. 4. Cambridge University Press, Cambridge.

28.Valles J., Garnatje T., Garcia S., Sanz M. and Ko-Robkov A. A. (2005) Chromosome numbers in the tribes Anthemideae and Inuleae (Asteraceae) in Kazakhstan. Botanical Journal of Linean Society 148: 77-85.

29.Volkova S. A. and oyko E. V. (1986) Chromosome numbers in some species of Asteraceae from the southern part of the Soviet Far East. Botanicheskii Zhurnal 71: 16-93.

30.Watanabe K. (2002) Index to chromosome numbers in Asteraceae. Available in http://www-asteraceae.cla.kobe-u.ac.jp/ index.html.

31.Watson L. E. (1754) Flora of North America, Tanacetum. Vol, 19, 20 and 21. Available in www.eFloras.org.

32.Yang Z., Zhang L., Zhao H., Yang R., Ding C., Zhou Y. and Wan D. (2009) Chromosome numbers of some species of Salvia (Lamiaceae) from the Sichuan Province, China. Nordic Journal of Botany 27: 287–291.

33.Yousefzadeh K. Hoshmand S. and Zinali H. (2010) Karyological study four speices of Anthemis from Iran. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 18(1): 55-62.

34.Zuo L. and Yuan Q. (2011) The difference between the heterogeneity of the centromeric index and intrachromosomal asymmetry. Plant Systematics and Evolution 297: 141–145.