Vitamin E Pretreatment of Mesenchymal Stem Cells: The Interplay of Oxidative Stress and Inflammation

Journal of Cell and Molecular Research

مقاله 7، دوره 11، شماره 2 - شماره پیاپی 22، خرداد 2020، صفحه 99-107   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v11i2.85668

نویسندگان

Shadi Mehrzad¹؛ Sepideh sadat Hosseini¹؛ Madjid Momeni-Moghaddam¹؛ Moien Farshchian²؛ Halimeh Hassanzadeh²؛ Mahdi Mirahmadi²؛ Fatemeh Sadeghifar¹؛ Hamid Reza Bidkhori^{* 2}

¹Department of Biology, Faculty of science, Hakim Sabzevari University, Sabzevar, Iran

²Stem Cells and Regenerative Medicine Research Department, Academic Center for Education, Culture and Research (ACECR)-Khorasan Razavi, Mashhad, Iran

چکیده

     Oxidative stress occurs as a result of breaking down the balance between oxidants (e.g., reactive oxygen species (ROS)) and antioxidants in cells. Several studies have shown that there is a close relationship between oxidative stress and inflammation at the sites of injury. Mesenchymal stem cells (MSCs) are exposed to endogenous and exogenous oxidants generated during their ex vivo expansion or following in vivo transplantation. α-tocopherol (vitamin E) is a fat-soluble compound known for its anti-oxidant and anti-inflammatory properties. In many studies, the immunomodulatory effects of vitamin E have been observed in vivo. This study aimed to determine whether pretreatment of MSCs with antioxidants like vitamin E, will enhance the anti-inflammatory and immunomodulatory properties of these cells. For this purpose, adipose-derived MSCs (ASCs) were treated with vitamin E (600 μM) for 48 h. Quantitative PCR (qPCR) experiments were performed to evaluate the expression of genes related to inflammation (IL-1β, IL-6, IL-17, IL-10) or immunomodulation (TSG-6, COX-2, TDO2, TGF-β1). Results indicated that vitamin E significantly increased the expression of COX-2, TSG-6, and IL-1β genes at the mRNA level compared with the control group, while it significantly decreased IL-6 and TGF-β expressions. No effect was observed for IL-17, IL-10, and TDO2 genes. These results suggest that in vitro preconditioning of ASCs with vitamin E may allow the cells to improve their anti-inflammatory and immunoregulatory capacities. Vitamin E pretreatment could lead to the improvement of their therapeutic abilities in conditions that are influenced by oxidative stress.

کلیدواژه‌ها

Immunology؛ Mesenchymal Stem Cells؛ English؛ vitamin E؛ Immunomodulation؛ oxidative stress؛ Preconditioning

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