تأثیر اسید‌‌ سالیسیلیک و تنش شوری بر جوانه‌زنی بذر و آنتی‌اکسیدان‌های فراسودمند در جوانه‌های کلم براکلی

علوم باغبانی

مقاله 8، دوره 28، شماره 3، آذر 1393، صفحه 388-398   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jhorts4.v0i0.42863

نویسندگان

مصباح بابالار¹؛ جواد هاشمی²؛ یونس مستوفی¹

¹دانشگاه تهران

²پژوهشکده بیوتکنولوژی منطقه مرکزی کشور

چکیده

سولفورافان ماده‌ای بسیار قوی در کنترل سرطان می‌باشد. این ماده در کلم براکلی تولید می‌شود و به نظر می‌رسد تنش شوری از طریق فعال کردن مقاومت اکتسابی سیستمیک باعث بیش‌تر شدن تولید سولفورافان گردد. با هدف به‌دست آوردن غلظت مناسب شوری برای تولید حداکثر سولفورافان، به‌همراه کنترل درصد جوانه‌زنی، تیمار با NaCl در 5 سطح0، 50، 100، 150، 200 میلی‌مولار به‌همراه اسیدسالیسیلیک (0 ، 100،200 میکرومولار) مورد آزمایش قرار گرفت. برای بررسی تأثیر تیمارها بر حداکثر درصد جوانه‌زنی، سرعت افزایش و زمان لگ، مدل لجستیک استفاده شد و سولفورافان و ویتامین ث با استفاده از HPLC اندازه‌گیری شدند. اسید سالیسیلیک مقاومت به شوری را افزایش داد. ترکیب اسید سالیسیلیک و کلرید سدیم غلظت سولفورافان و ویتامین‌ث را افزایش داد. تیمار100 میکرومولار اسید سالیسیلیک به‌همراه 100 میلی‌مولار کلرید سدیم بالاترین غلظت سولفورافان و ویتامین ث، به‌همراه بهترین فاکتورهای رشدی را ایجاد نمود.

کلیدواژه‌ها

هیدرکسی بنزوئیک اسید؛ کلرید سدیم؛ فاکتورهای جوانه‌زنی؛ سولفورافان؛ ویتامین ث

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