The effects of apple pulp and probiotic on performance, egg quality traits and blood parameters of laying hens

پژوهشهای علوم دامی ایران

Article 8, Volume 8, Issue 2 - Serial Number 26, June 2016, Pages 340-350   

Document Type: Poultry Nutrition

DOI: 10.22067/ijasr.v8i2.47865

Authors

Shabaz Noranian^* ; Ali Nobakht

Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Maragheh Branch, Maragheh, Iran

Abstract

Introduction Apple is one of the most important fruits that is produced in the large amount in Iran. It is a good source of vitamins and minerals and active fiber. Most of the apples that product in Iran are use in food industry for producing different kinds of apple juices. After Juicing, more than 20% of apple, remain as waste. The remained matter contain considerable amount of vitamins and minerals that usually found in fresh apple, moreover it is rich source of pectin and crude fiber. Generally this byproduct discharge to environment and cause some serious environmental problems. It is thought that use of apple pulp as a part of apple waste in laying hens diets not only prevent some environmental problems, but also can improve their performance, egg quality traits, and blood biochemical parameters and reduce the production cost. The current study has been designed to investigate these traits.
Materials and Methods This experiment was carried out on 192 Hi-line (W36) laying hens in a completely randomized design as (2*2) factorial arrangement with two levels of apple pulp (0 and 4%) and two levels of probiotic (protexin) (0 and 0.005%) in 4 treatments, 4 replicates and 12 birds per replicate for 12 weeks (65-76 weeks).
Results and Discussion Using apple pulp and probiotic in diets improved the egg production performance, egg quality traits and blood parameters of laying hens (P0.05). Probiotic improved egg weight, egg production percentage, egg mass, feed conversion ratio and Haugh unit. In interaction effects, using apple pulp and probiotic improved the performance and egg quality traits of laying hens. The highest egg weight, egg production, egg mass and the best feed conversion were obtained with diet containing 4% apple pulp and 0.005% probiotic. Also the highest amount of albumin, eggshell thickness and Haugh unit were observed with 4% apple pulp and 0.05% probiotic. Apple pulp decreased the blood levels of triglyceride, cholesterol and albumin and increased HDL (P>0.05). In interaction effect, using apple pulp with probiotic reduced the amount of triglyceride and albumin meanwhile the amount of blood cholesterol increased (P>0.05). Apple pulp contains considerable amount of essentials nutrients such as vitamins and minerals and secondary substances like antioxidants, crude fiber and pectin. Use of apple pulp in hens diets improved the digestive tract health and highly amount of these compositions transfer to birds body and improve their performance, egg quality and blood parameters. The high amount of dietary fiber in apple pulp may be the main reason of blood parameters improving. The fibrous diets not only caused the secretion of bile increase but also increased the amount of cholesterol excretion compared to normal condition. Probiotic created a good environment in digestive tract by decreasing the population of harmful microorganisms, increasing the number of gram positive microorganisms and reduced pH of digesta in gut which can have positive effects on performance of laying hens. In diets containing probiotic, only productive parameters improved, whereas egg quality and blood biochemical parameters were not affected, meanwhile incorporated probiotic in apple pulp treatments, effectively improved egg quality traits and blood biochemical parameters more than performance. Apple pulp contain some composition that their digestion have some difficulties for laying hens, so, incorporation of probiotics such as protexin in diets those containing highly amount of undesirable composition such as fiber and pectin, can have good effects on egg production, egg traits and can improve blood biochemical parameters and be useful for efficient use of apple pulp by hens
Conclusion It can be resulted that using 4% of apple pulp in laying hens not only had no adverse effects on performance, egg quality traits and blood biochemical parameters, but also could effectively improve most of them, in contrary with more fibrous feeds that increased the amount of feed intake in bird, using 4% apple pulp did not have significant effects on the amount of daily feed intake in laying hens. Using 0.005% of probiotic can improve the performance, egg traits and blood parameters in laying hens.

Keywords

Apple pulp; Blood metabolites; Egg quality traits; laying hens; Performance

References

1- Afshar Mazandaran, N., and A. Rajab. 2001. Probiotics and there using in poultry nutrition. Norbakhish Publisher, pp: 88-95. (In Persian)

2- Ahmad, G. A. 2004. Simultaneous use of apple pomace and molasses as a source of energy for broiler. Indian. Journal of Poultry Science. 39 (2): 179-181.

3- Amer, N., E. J. Al-Hilfy., and M. Al- Taie. 2011. Effect of apple- lite contained of apple fibers and gel pectin on body weight, lipid profiles, kidney function and histological structure of kidney in male albino rats. Danish Journa of Physiology Science, 8 (2): 178-187.

4- AOAC. 2002. Official Methods of Analysis of the Association of official analytical. Eds. Washington DC.

5- Bardos, L., and B. Bender. 2012. Effect of apple cider vinegar on plasma lipids (Model experiment in mice). Potravinarstvo, 6 (1): 1-4.

6- Carlm, P. 1984. Influence of caecectomy and source of dietary fiber of starch on excretion of endogenous amino acids by laying hens. British Journal of Nutrition, 51: 541-548.

7- Fanimo, A. O., O. O. Oduguwa., A. A. Alade., T. O. Ogunnaike., and A. K. Adesehinwa. 2003. Growth performance, nutrient digestibility and carcass characteristic of growing rabbits fed cashew apple waste, Livestock Research and Rural Development, 15 (8): 15-23.

8- Farkhoy, M., F, Sigharody., and F. Niknafas. 1994. Poultry breeding. Second Edition. Coasar Publication, pp: 150-266. (In Persian)

9- Ghaemi, H., A. Nobakht., and S. Razzagzadeh. 2014. The effect of apple pulp and multi enzyme on performance and blood parameters in native laying hens, Journal of Farm Animal Nutrition and Physiology, 9/1: 10-21.

10- Haddadin, M.S.Y., S. M., Abdulrahim., E. A. R. Hashlamoun., and R. K. Robinson. 1996. The effects of lactobacillus acidophilus on production and chemical composition of hen eggs. Poultry Science, 75: 491-494.

11- Kabir, S., M. M., Rahman, M. B. Rahman, and S. U. Ahmad. 2004. The dynamics of probiotics on growth performance and immune response in broiler. . Poultry Science, 3: 61-64.

12- Kalavathy, R., N. Abdullah., and S. Jalaludin, S. 2003. Effects of lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. Br. . Poultry Science, 44: 139 -144.

13- Khayat Nouri, M., and A. Kargari Rezapour. 2011. Effect of apple (Malus domestica) supplementation on serum lipids and lipoproteins level in cholesterol –fed male rat. Middlest Journal Science Research, 9 (6): 744-748.

14- Matoo, F. A., G. A. Beat., M. T. Banday., and T. A. S. Ganaie. 2001. Performance of broilers fed on apple pomace diets supplemented with enzyme (S). Indian Journal of Animal Nutrition, 18 (4): 349-352.

15- National Research Council, NRC. 1994. Nutrient requirements of poultry. 9th rev.ed. National Academy Press. Washington. DC.

16 Nazifi, S. 1997. Hematology and clinical biochemistry of birds. First Edition. Shiraz University Publicationو pp, 173-290. (In Persian)

17- Safamehr, A. R., and A. Nobakht. 2008. Effect of probiotic (Protexin) on performance, blood biochemical parameters and egg quality in laying hens. Journal of Argiculture Science, 4: 61-71. . (In Persian)

18- SAS Institute. 2005. SAS Users guide: Statistics. Version 9.12. SAS Institute Inc., Cary, NC. pp: 126-178.

19- Valizadeh, M. and M. Moghaddam. 1994. Experimental designs in agriculture (1). Pishtaz Ekem publication, pp: 75-100. (In Persian)

20- Yousefi, M., and K. Karkoodi. 2007. Effect of probiotic Thepax® and Saccharomyces cerevisiae supplementation on performance and egg quality of laying hens. International Journal of Poultry Science, 6: 28. 28.

21- Zafar, f., M. Idrees., and Z. Ahmad. 2005. Use of apple byproducts in poultry rations of broiler chicks in Karachi. Pakistan Journal of Physiology, 1: 13-17.