Effects of Organic and Chemical Fertilizers on Leaf Yield, Essential Oil Content and Composition of Lemon Verbena (Lippia citriodora Kunth)

علوم باغبانی

Article 2, Volume 30, Issue 2, April 2016, Pages 293-302   

Document Type: Research Article

DOI: 10.22067/jhorts4.v30i2.41574

Authors

Mohammadtaghi Ebadi; Majid Azizi¹; Fatemeh Sefidkon²; Noorollah Ahmadi^{* 3}

¹Ferdowsi University of Mashhad

²Institute of Forests and Rangelands, Tehran

³Tarbiat Modares University

Abstract

Introduction: Organic fertilizers with beneficial effects on soil structure and nutrient availability help maintain yield and quality, and they are less costly than synthetic fertilizers. Vermicompost and vermiwash are two organic fertilizers that they contain a biologically active mixture of bacteria, enzymes and phytohormones, also these organic fertilizers can supply the nutritional needs of plants. Lemon verbena (Lippia citriodora Kunth, Verbenaceae) is an evergreen perennial aromatic plant. The lemon-scented essential oil from the lemon verbena has been widely used for its digestive, relaxing, antimalarial and lemony flavor properties. In order to decrease the use of chemical fertilizers for reduction of environmental pollution, this research was undertaken to determine effects of vermicompost and vermiwash in comparison with chemical fertilizer on leaf yield, essential oil content and composition of lemon verbena.
Materials and Methods: A pot experiment based on a completely randomized design with six treatments and three replications on Lemon verbena was carried out in the experimental greenhouse of the Department of Horticulture Sciences, Tarbiat Modares University, 2012. Treatments consisted of 10, 20 and 30 % by volume of vermicompost and vermiwash (with an addition to irrigation in three steps, including: two weeks after the establishment of plants in pots, the appearing of branches and three weeks before harvest), complete fertilizer and control without any fertilizer. Each replication contained six pots and each pot contained one plant of Lemon verbena provided from Institute of Medicinal Plants, Karaj, therefore 108 pots were used in this experiment. The pots were filled up by a mixture contained 3/5 soil and 2/5 sand (v/v). After three months, plant aerial parts were harvested concomitantly at starting of the flowering stage. Aerial parts were dried at room temperature for 72 hours and dry weights of dried branches and leaves were measured. For isolation of essential oils, about 20 g of dried leaves (three replications of each treatment) was subjected to hydro-distillation for 2 h with a Clevenger apparatus. The extracted essential oils were dried over anhydrous sodium sulphate and then they analyzed by GC and GC/MS. The data were subjected to analysis of variance using SAS software and means compared with Duncan's multiple range test.
Results and Discussion: The results showed that differences between treated plants with organic fertilizers were not significant for aerial part yield, but leaf yield and essential oil content were affected by fertilizer type (p

Keywords

Citral; Medicinal plant; Vermicompost; Vermiwash

References

1- Adams R.P. 2001. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Crop, NewYork. 456 p.

2- Akbarinia A., Ghalavand A., Sefidkon F., Rezaei M.B., and Sharifi A. 2004. Study on the effect of different rates of chemical fertilizer, manure and mixture of them on seed yield and main, compositions of essential oil of ajowan (Trachyspermum copticum). Pajouhesh-Va-Sazandegi, 61: 32-41. (in Persian with English abstract)

3- Alavi L., Barzegar M., Jabbari A., and Naghdibadi H. 2011. The effect of heat treatment on chemical composition and antioxidant property of Lippia citriodora essential oil. Journal of Medicinal Plants, 10: 65-75.

4- Anwar M., Patra D.D., Chand S., Alpesh K., Naqvi A.A., and Khanuja S.P.S. 2005. Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation, and oil quality of French basil. Communications in Soil Science and Plant Analysis, 36 (13-14): 1737-1746.

5- Atiyeh R.M., Arancon N.Q., Edwards C.A., and Metzger J.D. 2001. The influence of earthworm processed pig manure on the growth and productivity of marigolds. Bioresource Technology, 81 (2): 103-108.

6- Atiyeh R.M., Subler S., Edwards C.A., Bachman G., Metzger J.D., and Shuster W. 2000. Effects of vermicomposts and composts on plant growth in horticultural container media and soil. Pedobiologia, 44 (5): 579-590.

7- Ayyobi H., Peyvast G.A., and Olfati J.A. 2013. Effect of vermicompost and vermicompost extract on oil yield and quality of peppermint (Mentha piperita L.). Journal of Agricultural Sciences, 58 (1): 51-60.

8- Azizi M., Baghani M., Lackzian A., and Aroiee H. 2007. Effect of different level of vermicompost and vermiwash spraying on morphological traits and essential oils content of Ocimum basilicum. Agricultural Sciences and Technology, 21: 41-52. (in Persian with English abstract)

9- Bremness L. 1999. Herbs. Eyewitness Handbook, London, 176 p.

10- Carnat A., Carnat A.P., Fraisse D., and Lamaison J.L. 1999. The aromatic and polyphenolic composition of lemon verbena tea. Fitoterapia, 70: 44-49.

11- Darzi M.T., Hadjseyed Hadi M.R., and Rejali F. 2011. Effects of vermicompost and phosphate biofertilizer application on yield and yield components in Anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants, 26: 452-465. (in Persian with English abstract)

12- Duarte M.C., Figueira G.M., Sartoratto A., Rehder V.L., and Delarmelina C. 2005. Anti-Candida activity of Brazilian medicinal plants. Journal of Ethnopharmacology, 97 (2): 305-311.

13- Duschatzky C.B., Martinez A.N., Almeida N.V., and Bonivardo S.L. 2004. Nematicidal activity of the essential oils of several Argentina plants against the root-knot nematode. Journal of Essential Oil Research, 16 (6): 626-628.

14- Ghasemi Dehkordi N. 2000. Iranian Herbal Pharmacopeia. Ministry of Health and Medical Education publication, Tehran. 795p. (in Persian)

15- Ghazi Manas M., Banj Shafiee Sh., Hajseyed Hadi M.R., and Darzi M.T. 2013. Effects of vermicompost and nitrogen on qualitative and quantitative yield of chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants, 29: 269-280. (in Persian with English abstract)

16- Jahan M., Amiri M.B., Shabahang J., and Tahami M.K. 2013. The effects of simultaneous application of different organic and biological fertilizers on quantitative and qualitative characteristics of medicinal pumpkin (Cucurbita pepo L.). Iranian Journal of Field Crops Research, 11: 73-87. (in Persian with English abstract)

17- Kiafar R., Akbarzadeh M., and Mahboub Khommami A. 2013. Investigation of the effect of some organic fertilizers on the oil of lemon verbena (Lippia citriodora L.) and its antibacterial effects. International Journal of Farming and Allied Sciences, 2 (20): 866-871.

18- Kintzios S.E. 2002. Oregano: The Genera Origanum and Lippia. CRC press, NewYork. 296 p.

19- Lopez A.G., Theumer M.G., Zygadlo J.A. and Rubinstein H.R. 2004. Aromatic plants essential oils activity on Fusarium verticillioides Fumonisin B (1) production in corn grain. Mycopathologia, 158 (3): 343-349.

20- Mafakheri S., Omidbaigi R., Sefidkon F. and Rejali F. 2012. Effect of vermicompost, biophosphate and azotobacter on quantity and quality of essential oil of Dracocephalum moldavica L. Iranian Journal of Medicinal and Aromatic Plants, 27: 596-605. (in Persian with English abstract)

21- Moradi R., Nasiri Mahallati M., Rezvani Moghaddam P., Lakzian A., and Nejad Ali A. 2011. The effect of application of organic and biological fertilizers on quantity and quality essential oil of Foeniculum vulgare Mill. Journal of Horticulture Science, 25: 25-33. (in Persian with English abstract)

22- Mozaffarian V. 2009. Dictionary of Iranian Plant Names: Latin-English-Persian. Farhang Moaser Publication, Tehran. 671p. (in Persian)

23- Nath G., and Singh K. 2011. Effect of foliar spray of biopesticides and vermiwash of animal, agro and kitchen wastes on soybean (Glycine max L.) crop. Botany Research International, 4 (3): 52-57.

24- Nath G., and Singh K. 2012. Effect of vermiwash of different vermicomposts on the kharif crops. Journal of Central European Agriculture, 13(2): 379-402.

25- Nemati H., Azizi M., Mohammadi S., and Karimpour S. 2013. The study on the effect of spraying with different concentrations of vermicompost extract (vermiwash) on the morphological traits, yield and percentage of essential oil of lemon balm (Melissa officinalis L.). Journal of Horticulture Science (Agricultural Sciences and Technology), 27: 411-417. (in Persian with English abstract)

26- Ohno T., Kita M., Yamaoka Y., Imamura S., Yamamoto T., Mitsufuji S., Kodama T., Kashima K., and Imanishi J.O. 2003. Antimicrobial activity of essential oils against Helicobacter pylori. Helicobacter, 8 (3): 207-215.

27- Omidbaigi R. 2006. Production and Processing of Medicinal Plants. Vol 1. Behnashr Publication, Mashhad. 397p. (in Persian)

28- Ono M., Oda E., Tanaka T., Iida Y., Yamasaki T., Masuoka C., Ikeda T., and Nohara T. 2008. DPPH radical-scavenging effect on some constituents from the aerial parts of Lippia triphylla. Journal of Natural Medicines, 62 (1): 101-106.

29- Sahoo S. 2001. Conservation and Utilization of Medicinal and Aromatic Plants. Allied publishers, New Delhi, 423 p.

30- Santos-Gomes P.C., Fernandes-Ferreira M., and Vicente A.M.S. 2005. Composition of the essential oils from flowers and leaves of Vervain (Aloysia triphylla Britton) Grown in Portugal. Journal of Essential Oil Research, 17 (1): 73-78.

31- Valentão P., Fernandes E., Carvalho F., Andrade P.B., Seabra R.M., and Bastos M.L. 2002. Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biological & Pharmaceutical Bulletin, 25 (10): 1324-1327.