Isolation and identification of antioxidants components From Cumin seed (Cuminum cyminum)

مجله پژوهشهای علوم و صنایع غذایی ایران

Article 2, Volume 12, Issue 6 - Serial Number 42, January and February 2017, Pages 742-749   

Document Type: Research Article-en

DOI: 10.22067/ifstrj.v1395i0.53312

Authors

Soudabeh Einafshar^{* 1}; Hashem Pourazerang²; Reza Farhoosh³; Javad Asili⁴

¹Khorasan Razavi Agricultural and Natural Resources Research and Education Center

²Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran.

³Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran.

⁴Department of pharmacognosy

Abstract

Natural materials are complicated compositions; therefore, a rapid screening of the active antioxidants is being challenged in the literature. Different polarity solvents were applied to isolate four fractions (F1, F2, F3 and F4) of methanolic extract of Cuminum cyminum. Their anti-oxidative properties were tested using radical scavenging and FRAP assays. F3 (with IC50=0.006mg/mL and FRAP= 521.95 mmolFe2+/L) was significantly the most active fraction. Guided isolation through bio-autography on TLC using 1, 1- diphenyl- 2 – picryl- hydrazyl radical (DPPH) as a detection reagent led to the isolation of two antioxidant compounds from F3. F3 was injected to a preparative HPLC with the proper mobile phase (acetonitril: methanol/ water) and isolated two main compounds. These compounds were identified as Luteolin 7 glugoside and Apigenin 7 glucoside by means of 1HNMR and 13CNMR and compare them with references.

Keywords

Antioxidant; Apigenin; Cuminum cyminum; Luteolin

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