شناسایی و بررسی مولکولی ویروس موزائیک نیشکر (Sugarcane mosaic virus; SCMV) در استان مازندران

پژوهش های حفاظت گیاهان ایران

مقاله 3، دوره 30، شماره 4، اسفند 1395، صفحه 639-645   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v30i4.50484

نویسندگان

زهره مرادی^{* 1}؛ محسن مهرور¹؛ احسان نظیفی²؛ محمد زکی عقل¹

¹دانشگاه فردوسی مشهد

²دانشگاه مازندران

چکیده

ویروس موزائیک نیشکر (Sugarcane mosaic virus; SCMV) یکی از بیمارگرهای بسیار مهم و خسارت زا در مناطق کشت ذرت و نیشکر در دنیا است. به منظور ردیابی و شناسایی این ویروس، در سال های 1393-1392 تعداد 45 نمونه مشکوک به آلودگی از مزارع نیشکر و ذرت در استان مازندران جمع آوری شد. در واکنش زنجیره ای پلیمراز با نسخه برداری معکوس (Reverse transcription-PCR, RT-PCR) با استفاده از یک جفت آغازگر اختصاصی مربوط به ژن پروتئین پوششی، قطعه ای به طول900 جفت باز در 35 نمونه تکثیر گردید. محصول PCR مربوط به چهار جدایه پس از همسانه سازی در ناقلpTG19-T ، توالی یابی شده و با سایر توالی های موجود در بانک ژن مقایسه گردید. در آنالیز فیلوژنتیکی، توالی نوکلئوتیدی 36 جدایه ویروس موزائیک نیشکر در دو گروه ( I وII ) و چهار زیر گروه قرار گرفتند و جدایه های مازندران نیز در کنار جدایه های خوزستان و مصر در زیرگروه IA قرار گرفتند. آنالیز فیلوژنتیکی جدایه های تعیین توالی شده در ناحیه ژنی پروتئین پوششی نشان داد که چهار جدایه مازندران با سایر جدایه‌های ویروس موزائیک نیشکر بین 78 تا 99 % در سطح نوکلئوتیدی مشابهت دارند که بیشترین شباهت با جدایه EGY7-1 از مصر (بین 6/97 تا 99 %) و جدایه های khzQ86و khzL66 از خوزستان در ایران (بین 6/96 تا 8/98 %) و کمترین شباهت با جدایه BD8 از چین (بین 78 تا 8/78 %) می باشد. همچنین تشابه توالی نوکلئوتیدی و آمینواسیدی این چهار جدایه با یکدیگر به ترتیب بین 9/98-97 و 100-3/99 % تعیین گردید. این اولین گزارش از وجود SCMV در استان مازندران براساس ژن پروتئین پوششی است.

کلیدواژه‌ها

آنالیز فیلوژنتیکی؛ ذرت و نیشکر؛ ژن پروتئین پوششی؛ مازندران؛ ویروس موزائیک نیشکر

مراجع

1- Adams M.J., Antoniw J.F., and Fauquet C.M. 2005. Molecular criteria for genus and species discrimination within the family Potyviridae. Archives of Virology, 150: 459–479.

2- Alegria O.M., Royer M., Bousalem M., Chatenet M., Peterschmitt M., Girard J-C., and Rott P. 2003. Genetic diversity in the coat protein coding region of eighty-six Sugarcane mosaic virus isolates from eight countries, particularly from Cameroon and Congo. Archives of Virology, 148: 357–372.

3- Amiri F., and Izadpanah K. 1993. Purification, serology and transmission of Sugarcane mosaic virus in Khuzestan. p. 126. Proceedings of the 11th Iranian plant protection congress, 23-27 Aug. 1993. Rasht, Iran. (In Persian)

4- Atreya P.L., Lopez-moya J.J., Chu M., Atreya C.D., and Pirone T.P. 1995. Mutational analysis of the coat protein N-terminal amino acids involved in potyviral transmission by aphids. Journal of General Virology, 76: 265–270.

5- Barat Shooshtari M., Ahmadian S., Asfia Gh. Sugarcane in Iran, (Aeej press, Tehran, 2008), 337 p (In Persian)

6- Berger P.H., Wyatt S.D., Shiel P.J., Silbernagel M.J., and Druffel K. 1997. Phylogenetic analysis of the Potyviridae with emphasison legume-infecting potyviruses. Archives of Virology, 142: 1979±1999.

7- Berger P.H., Adams M.J., Brunt A.A., Hill J.H., Hammond J., Jordan R.L., Morales R.J., Ohki S.T., Rybicki E., Uyeda I., and Vetten H.J. 2005. The Potyviridae. In: Fauquet C, Mayo M, Maniloff F, Desselberger U, Ball L (eds) Virus taxonomy-classification and nomenclature of viruses, 8th report of the ICTV. Elsevier, San Diego, pp 819–841.

8- Brandes E.W. 1919. The mosaic disease of sugarcane and other grasses. Technical Bulletin, US Department of Agriculture, 829, 1-26.

9- Chen J., Chen J., and Adams M.J. 2002. Characterization of potyviruses from sugarcane and maize in China. Archives of Virology, 147: 1237-1246.

10- Dolja V.V., Haldeman R., Robertson N.L., Dougherty W.G., and Carrington J.C. 1994. Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus in plants. EMBO Journal, 13: 1482–1491.

11- Dujovny G., Sasaya T., Koganesawa H., Usugi T., Shohara K., and Lenardon S.L. 2000. Molecular characterization of a new potyvirus infecting sunflower. Archives of Virology, 145: 2249–2258.

12- Fan Z.F., Wang W.J., Jiang X., Liang X.M., Wang F.R., and Li H.F. 2004. Natural infection of maize by Pennisetum mosaic virus in China. Plant Pathology, 53: p 796.

13- Fuchs E., and Grüntzig M. 1995. Influence of Sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) on the growth and yield of two maize varieties. Journal of plant disease and Protection, 102: 44-50.

14- Ghasemi S. 2005. Grouping of poaceae potyviruses in Iran on the basis of serological relationship and sequence of the 3' region of the genome and study of VPg-HC-Pro interaction of Potato virus Y using yeast two hybrid system. PhD Thesis, Shiraz University.

15- Gomeza M., Ragob A.M., and Serino G. 2009. Rapid identification of viruses causing sugarcane mosaic by direct sequencing of RT-PCR products from crude extracts: A method for large scale virus surveys. Journal of Virological Methods, 157: 188–194.

16- Goodman B.S. 1999. A study of South African strains of Sugarcane Mosaic Potyvirus (SCMV) identified by sequence analysis of the 5′ region of the coat protein gene. M.S. thesis, Department of Biotechnology, Durban University of Technology, Durban, South Africa.

17- Gotz R., and Maiss E. 2002. The complete sequence of the genome of Cocksfoot streak virus (CSV), a grass infecting Potyvirus. Archives of Virology, 147: 1573–1583.

18- Grisham M.P. 2000. Mosaic. In: Rott P, Bailey RA, Comstock JC, Croft BJ, Saumtally AS (ed) A guide to sugarcane diseases. Cirad/Issct, Montpellier, France, pp 249–254.

19- Hull R. 2014. Plant virology (5th ed.). New York: Academic Press.1098 p.

20- Janson B.F., and Ellett C.W. 1963. A new corn disease in Ohio. Plant Disease reporter journal, 47: 1107-1108.

21- Koike H., and Gillaspie A.G. 1989. Mosaic. In: Diseases of Sugarcane-Major Diseases. (Ricaud, C., Eagan, B. T. and Gillaspie, A. G., eds.) Sientific Publishers, Amsterdam. Pp: 301-322.

22- Masumi M., Zare A., Ghasemi S., and Izadpanah K. 2004. Relationship between two dominant strains of Sugarcane mosaic virus (SCMV) from Khuzestan and other SCMV strains based on nucleotide sequence of 3ʹ region of the genome. p. 314. Proceedings of the 16th Iranian plant protection congress, Vol. 2. 28 Aug-1 Sep. 2004. Tabriz, Iran. (In Persian).

23- Masumi M., Zare A., and Izadpanah K. 2006. Revision of Sugarcane mosaic virus strain grouping in the world on the basis of phylogenetic analysis of N-terminus of coat protein gene. Pp: 175-176. Proceedings of the 3rd Iranian congress of Virology, Jan 2006 Tehran, Iran. (In Persian)

24- Masumi M., Zare A., and Izadpanah K.A. 2007. Taxonomic position of two Iranian isolates of Sugarcane mosaic virus (SCMV) based on sequence of the 3'-region of the genome. Iranian Journal of Plant Pathology, 43: 1 (169); 1-16. (in Persian with English abstract)

25- Mirghasempour A., Hosseini A., Babaeizad V., and Hosseini S. 2014. Identification of causal agent of mosaic disease in sugarcane fields of Mazandaran province, Iran Proceedings of the 2nd national conference on applied researches in agriculture sciences, Tehran, Iran. (in Persian with English abstract)

26- Mohammadi M.R., Koohi-Habibi M., and Mosahebi G. 2009. Identification of the Prevalent Potyvirus on Maize in Corn Fields of Tehran Province and a Study on some of its Properties. Iranian Journal of Plant Protection Science, 40(1):43-53. (in Persian with English abstract)

27- Perera M.F., Filippone M.P., Ramallo C.J., Cuenya M.I., Garcia M.L., Ploper L.D., and Castagnaro A.P. 2009. Genetic diversity among viruses associated with sugarcane mosaic disease in Tucuman, Argentina. Phytopathology, 99(1): 38-49.

28- Seifers D.L., Salomon R., Marie-Jeanne V., Alliot B., Signoret P., Haber S., Loboda A., Ens W., She Y.M., and Standing K.G. 2000. Characterization of a novel potyvirus isolated from maize in Israel. Phytopathology, 90: 505-513.

29- Shukla D.D., Tosic M., Jilka J., Ford R.E., Toler R.W., and Langham M. 1989. Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology, 79: 223-229.

30- Shukla D.D., Ward C.W., and Brunt A.A. 1994. The Sugarcane mosaic virus subgroup. In: The Potyviridae. CAB International,Wallingdorf, United Kingdom, pp. 360–371.

31- Tamura K., Stecher G., Peterson D., Filipski A., and Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30: 2725-2729.

32- Wu L., Zu X., Wang S., and Chen Y. 2012. Sugarcane mosaic virus – Long history but still a threat to industry. Crop Protection, 42: 74-78.

33- Xu D.L., Park J.W., Mirkov T.E., and Zhou G.H. 2008. Viruses causing mosaic disease in sugarcane and their genetic diversity in southern. China. Archives of Virology, 153: 1031-1039.

34- Yang Z.N., and Mirkov T.E. 1997. Sequence and relationships of Sugarcane mosaic and sorghum mosaic virus strains and development of RT-PCR-based RFLPs for strain discrimination. Phytopathology, 87: 932–939.

35- Yousefi M. 2009. A comprehensive description of Agriculture, Agricultural Engineering (breeding, agriculture, science and technology of seed, ecological agriculture). Arshad press. 254 p.

36- Zhu M., Chen Y., Ding X-S., Webb S. L., Zhou T., Nelson R. S., and Fan Z. 2014. Maize elongin C interacts with the viral genome-linked protein, VPg, of sugarcane mosaic virus and facilitates virus infection. New Phytologist, 203 (4): 1291-1304.