شناسایی جهش‌های FecXI، FecXH و FecXB در ژن BMP15 در گوسفند نژادهای لک قشقایی و بهمئی در استان کهگیلویه و بویر احمد

پژوهشهای علوم دامی ایران

مقاله 4، دوره 11، شماره 2 - شماره پیاپی 38، تیر 1398، صفحه 247-257   

نوع مقاله: علمی پژوهشی- ژنتیک و اصلاح دام و طیور

شناسه دیجیتال (DOI): 10.22067/ijasr.v1397i1.70188

نویسندگان

مصطفی محقق دولت آبادی^* ؛ اسما مرادعلی‌پور؛ فرخنده منصوری؛ فرنگیس فتاحی؛ زینب صالحی

دانشگاه یاسوج

چکیده

نرخ باروری یکی از صفات مهم اقتصادی در گوسفند است، که تحت تأثیر ژنتیک و محیط می­باشد. ژنBMP15  یکی از ‌ژن‌های موثر بر این صفت می‌باشد و جهش­های مختلف در این ژن باعث تغییر راندمان صفات تولیدمثلی و رشد در گوسفند می‌شود. چنانکه باروری بالا در گوسفندان به علت جهش در جایگاه BMP15 با منشأ اووسیتی (ژن فاکتور رشد) شناخته شده است. در این مطالعه از روش PCR-RFLP برای شناسایی چند شکلی‌ جایگاه‌های ژنی FecXI، FecXH وFecXB  استفاده شد. بدین منظور از تعداد 92 رأس میش نژاد لک قشقایی و بهمئی به طور تصادفی خون‌گیری صورت گرفت و استخراج DNA با استفاده از کیت استخراجDNA  انجام شد. قطعات مورد نظر با استفاده از آغازگرهای اختصاصی (قطعه bp 204 از FecXI، قطعه bp 235 از FecXH و قطعه bp 153 از ژن FecXB)، و توسط واکنش زنجیره پلی‌مراز (PCR) تکثیر گردید. پس از مجاورت محصولات PCR با آنزیم­های اختصاصی (XbaI، SpeI وDdeI)، مشاهده گردید که همه قطعات FecXI و FecXH فاقد جایگاه برش برای آنزیم هضم‌کننده بوده و تنها یک الگوی باندی در آن­ها مشاهده شد. این مطالعه تنها وجود آلل­های تیپ وحشی را تأیید کرد. تمامی نمونه‌ها دارای ژنوتیپ هموزیگوت بودند و جهش اینوردل و هانا (جهشی که در حالت هتروزیگوت باعث افزایش میزان تخمک‌گذاری می‌شود) در آن­ها یافت نشد. براساس نتایج این مطالعه، ژن BMP15 نمی­تواند تأثیری بر میزان چند‌قلوزایی در گوسفندان مورد مطالعه داشته باشد، بنابراین باید به دنبال جهش­ها یا ژن­های دیگری بود.

کلیدواژه‌ها

پلی مورفیسم؛ دو‌قلوزایی؛ ژن BMP15؛ گوسفند؛ PCR-RFLP

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