The Effect of Mechanical Stress on Some Morpho-Physiological and Biochemical Characteristics of Coleus blumei Benth.

علوم باغبانی

Article 12, Volume 32, Issue 4, March 2019, Pages 629-640   

Document Type: Research Article

DOI: 10.22067/jhorts4.v32i4.72467

Authors

Atefeh Safaeifar; Abdolhossein Rezaei Nejad^* ; Sadegh Mousavifard; Faizollah Shahbazi

Lorestan University

Abstract

 
Introduction: Coleus (Coleus blumei Benth.) from Laminaceae family is an herbaceous, fast growing plant with colorful and decorative leaves which is used as an ornamental and pot plants. Producing short and compact potted plants is valuable in floral industry. A chemical procedure has long been used for this purpose. However, chemical procedure is environmentally unsafe, while it is expensive. Therefore, researches are carried out to find cheaper and safer methods. Several researches have been done on height control, such as genetic manipulation, temperature management, light quality manipulation, controlled water deficit and withholding nutrients. Controlled mechanical stresses are included the potential non-chemical methods. In the present research, using a laboratory vibration simulator, the effects of vibration stress with different frequencies and durations had been examined on growth control of coleus.
Material and Methods: The experiment was conducted at the research greenhouse of Lorestan University, Iran in 2017. Uniform rooted cuttings of Coleus were transplanted in plastic pots (15 cm diameter and height) that filled with equal proportion of soil, sand and cow manure. The experimental design was a factorial based on a completely randomized design with three replications. After plant establishment, the treatments were applied every morning, with 7.5, 10 and 12.5 Hz frequencies and 0, 5 and 10 min durations, using a laboratory vibration simulator. Treatments application lasted for four weeks and then, plant height, stem diameter, total number of leaves per plant and leaf area, total number and length of side shoots, root volume and length, leaf, stem and root fresh and dry weights, relative water content, electrolyte leakage and the amounts of photosynthetic pigments were assessed.
Results: The results showed that interaction effects of frequency and duration of mechanical stress were significant at the 0.01 level in plant height, the total number and length of side shoots, root volume, leaf, stem and root fresh and dry weights, relative water content, the amounts of photosynthetic pigments, while it was not significant on electrolyte leakage. The main effects of duration of mechanical stress were significant at the 0.01 level in total number of leaves per plant and leaf area. Mean comparisons showed that with increasing frequency and duration of mechanical stress, plant height, total number of leaves per plant and leaf area, the total number and length of side shoots, root volume and length, leaf, stem and root fresh and dry weights and relative water content decreased. The shortest plant height was found in plants that treated with 12.5 frequency for 10 min which was 31% shorter than control plants. The main effects of frequency and duration of mechanical stress were significant at the 0.01 level in stem diameter, while their interaction effect was not significant. Stem diameter reduced with increasing stress duration. The stress effect on stem diameter was similar in 7.5 and 10 Hz frequencies. However, stem diameter was lower in plants treated with 12.5 Hz. Mechanical stress decreased the total number of leaves per plant and leaf area and the effects of 5 and 10 min duration were similar. With increasing frequency and duration of mechanical stress, the amounts of photosynthetic pigments increased; so that, the highest amount of those were found in plants that treated with 12.5 Hz frequency for 10 min.
Discussion: According to the results of the present research, vibration stress could control plant growth in Coleus. Stress treatments with all frequencies and durations, decreased plant height and other growth parameters i.e. the total number of leaves per plant. Leaf area, root length, plant biomass and relative water content were also decreased, while, electrolyte leakage was not significantly affected. At the same time, mechanical stress increased the amounts of photosynthetic pigments. Plants treated with 7.5 Hz frequency for 5 min showed 16% shorter plant height compared with controls, while other plant growth characteristics including stem diameter, leaf area, electrolyte leakage, MDA content and the amounts of photosynthetic pigments were similar to those in control plants. Therefore, this treatment could be recommended as a good vibration treatment for practical use. However, based on the production conditions, other vibration frequencies and durations could be chosen. In total, vibration stress using a vibration simulator could be a proper method for producing short and compact pot plants due to its cheapness and being easy to use and an environmentally friendly method. 

Keywords

Coleus; growth control; Plant height; vibration stress

References

1- Al-khassawneh M.N., Karam S.N., and Shibli R.A. 2006. Growth and flowering of iris (Iris nigricans Dinsm). Following treatment with plant growth regulators. Journal of Scientia Horticulturae, 107: 187-193.

2- Alvarez S., Navarro A., Banon S., and Sanchez-Blanco M.J. 2009. Regulated deficit irrigation in potted Dianthus plant: Effects of severe and moderate water stress on growth and physiological responses. Journal of Scientia Horticulturae, 122: 579-585.

3- Autio J., Voipo I., and Koivunen T. 1994. Responses of aster, dusty miller, and petunia seedlings to daily exposure to mechanical stress. HortScience, 29: 1449–1452.

4- Banon S., Gonzalez A., Cano E., Franco J., and Ferandez J. 2002. Growth, development and color response of potted Dianthus caryophyllus cv. Mondrian to paclobutrazol treatment. Journal of Scientia Horticulturae, 94: 371-377.

5- Barker J.E. 1979. Growth and Wood properties of Pinus radiata in relation to applied ethylene. New Zealand Journal of Forest Science, 9: 15-19.

6- Bi G., Scagel C.F., and Harkess R. 2008. Rate of Nitrogen fertigation during vegetative growth and spray applications of urea in the fall alters growth and flowering of florists’ Hydrangeas. Journal of Hort Science, 43: 472-477.

7- Biddington N.L., and Dearman A.S. 1978. The effects of mechanically-induced stress on the growth of water and nutrient deficient lettuce and cauliflowers seedlings, Annals of Botany, 59: 679-683.

8- Biro R.L., and Jaffe M.J. 1984. Thigmorphogenesis: ethylene evolution and its role in the changes observed in mechanically perturbed bean plants. Physiologia Plantarum, 62: 289-296.

9- Bjerregaard Lund J., Blom T., and Mazanti Aaslyng J. 2007. End-of-day lighting with different Red/Far-red ratios using light-emitting diodes affects plant growth of Chrysanthemum × morifolium ramat. ‘Carlo charm’. Journal of HortiScience, 42: 1609-1611.

10- Bjorkman T. 1999. Dose and Timing of Brushing to Control Excessive Hypocotyl Elongation in Cucumber Transplants. Hortechnology, 2: 224-226.

11- Boeger M.R.T., and Poulson M.E. 2003. Morphological adaptations and photosynthetic rates of amphibious Veronica anagallis-aquatica L. (Scrophulariaceae) under different flow regimes. Aqua. Bot., 75: 123-135.

12- Brotton C.J., and Cole C.J. 2009. Brushing Using a Hand Coated With Body Lotion or in a Latex Glove Decreases African violet Plant Quality and Size. Journal of Hort Technology, 190. pp. 613-616.

13- Buege J.A., and Aust SD. 1978. Microsomal lipid peroxidation. Methods Enzyme, 52: 302-310.

14- Burnett S., Lersel M., and Thomas P. 2005. PEG-8000Alters Morphology and Nutrient concentration of Hydroponic Impatiens. Journal of Hort Science, 40: 1768-1772.

15- Chia P-L., and Kubota C. 2010. End-of-day Far-red light quality and dose requirements for tomato rootstock hypocotyl elongation. Journal of HortScience, 45: 1501-1506.

16- Dhopte A.M., and Livera M.M. 2002. Principles and techniques for plant scientist:

17- Erner Y., and Jaffe M.J. 1982. Thigmomorphogenesis: the involvement of Auxin and Abscisic Acid in Growth retardation due to Mechanical perturbation. Journal of Plant & Cell physiol, 23: 935-941.

18- Garner L.C., and Langton F.A. 1997. Brushing pansy (Viola tricolor) transplants: a flexible, effective method for cotrolling plant size, Scientia Horticulture, 70: 187-195.

19- Graham T., and Wheeler R. 2017. Mechanical stimulation modifies canopy architecture and improves volume utilization efficiency in bell pepper: implications for bioregenerative life-support and vertical farming, open Agriculture, 42-51.

20- Heuchert J.C., Marks J.S. and Mitchel C.A. 1983. Strengthening of tomato shoots by gyratory shaking. Journal of the American Society of Horticultural Science, 10: 801-805.

21- Jaffe M.J., Biro R., and Bridle K. 1980. Thigmomorphogenesis: calibration of the parameters of the sensory function in beans, Physio. Plant. 49: 410-416.

22- Jones R.S., and Mitchell C.A. 1988. A marking device for the identification and measurement of growth zones in seedlings with minimum mechanical stress. Annuals of Applied Biology, 113: 437-441.

23- Jones R.S., Goe L.L., Montgomery L., and Mitchell C.A. 1990. Seismic stress response of soybean to different photosynthetic photon flux. Annuals of Botany, 66: 617-622.

24- Kafei M., Burzoei A., Salehei M., Kamandei A., Maasumei A., and Nabati J. 2009. Physiology of Environmental Stresses in Plants. Publications University of Mashhad.

25- Kafei M., and Damghanei A. 2002. The Mechanisms of Resistance of Plants to Environmental Stresses (translation). Ferdowsi University of Mashhad Publishing house.

26- Khajepoor R., Kafei M., Nezamei A., and Khazaei H. 2017. The Effect of Wind Mechanical Stress on Some Morphologicall Traits of two Semi-Dwarf and Normal Wheat (Triticum aestivum) cultivars. Journal of Crop Production, 10: 101-114.

27- Khalighi A. 1997. Potting and Breeding Ornamental Plants of Iran. Ruzbehan Publications.

28- Latimer J., and Mitchell C.A. 1988. Effects of Mechanical stress or Abscisic Acid on Growth, water status and leaf Abscisic Acid content of Eggplant seedlings. Journal of Scientia Horticulturae, 36: 37-46.

29- Latimer J.G., Pappas T., and Mitchel C.A. 1986. Growth responses of eggplant to mechanical stress in greenhouse and outdoor environments. Journal of the American Society for Horticultural Science, 111: 694-698.

30- Lichtenthaler H.K. 1987. Chlorophylls and cartenoides: pigments of photosynthetice bio-membranes. In: Method in Enzymol. (eds.s.p.colowick and N.O.Kaplan) Academic press. NewYork, 48: 350-382.

31- Lindberg S., and Petterson S. 1985. Effects of mechanical stress on uptake and distribution of nutrients in barley. Plant and Soil, 83: 295-309.

32- Lykas Ch., and Papafotiou M. 2008. Gardenia jasminoides Height control using a photoselective polyethylene film. Journal of Hort science, 43: 2027-2033.

33- Mitchell C.A. 1977. Influence of mechanical stress on Auxin-stimulated growth of excised pea stem section. Physiologia Plantarum, 41: 129-134.

34- Nagpaly A., Singh B., Sharma S., Rani G., and Virk G. 2008. Coleus spp: micropropagation and in vitro production of secondray metabolites- Global science books, 2: 1-17.

35- Pappas T., and Mitchel G.A. 1985. Effects of seismic stress on the vegetative growth of Glycine max (L.) Merr. cv. Wells II. Plant Cell and Environment, 8: 143-148.

36- Pontinen V., and Voipio I. 1992. Different Methods of Mechanical stress in controlling the Growth of Lettuce and Cauliflower seedling. Journal of Acta Agric. Scand., Sect. B, Soil and Plant Sci. 42: 246-250.

37- Sone K., Noguchi K., and Terashima I. 2006. Mechanical and ecophysiological significance of the form of a young Acer rufinerve tree: vertical gradient in branch mechanical properties. Tree Physiology, 26: 1549-1558.

38- Suge Hiroshi. 1978. Growth and gibberellin production in Phaseolus vulgaris as affected by mechanical stress, plant & cell physio, l: 303-310.

39- Telewski F., and Jaffe M.J. 1986. Thigmomorphogenesis: the role of ethylene in the response of Pinus taeda and Abies fraseri to mechanical perturbation. Physiologia Plantarum, 66: 227-233.

40- Wang Y-H., He W-M., Dong M., Yu F-H., Zhang L-L., Cui Q-G., and Chu Y. 2008. Effects of shaking on the Growth and Mechanical properties of Hedysarum leave may be independent of water regimes. Journal of Plant Sci. 169: 503-508.

41- Yamasaki S., and Dillenburg L.C. 1999. Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilian Fisiologia Vegetal, 11: 69-75.