ارزیابی اثر اسید لاکتیک بر ویژگی‌های بافتی و الگوی پروتئولیز عضله دو سر ران شتر یک کوهانه

نشریه پژوهشهای علوم و صنایع غذایی ایران

مقاله 4، دوره 15، شماره 1 - شماره پیاپی 55، فروردین و اردیبهشت 1398، صفحه 37-54   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ifstrj.v0i0.64625

نویسندگان

مهسان ایرجی فر¹؛ محمد جواد وریدی^{* 1}؛ مهدی وریدی¹؛ یونس زاهدی دیزج یکان²

¹گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران.

²گروه آموزشی علوم و مهندسی صنایع غذایی، دانشگاه محقق اردبیلی، ایران.

چکیده

در این تحقیق اثر ماریناد اسید لاکتیک ( در چهار غلظت صفر، 5/0، 1 و 5/1 درصد) وکلرید سدیم (2 درصد) طی زمان‌های غوطه‌وری صفر، 24، 48 و 72 بر ویژگی‌های بافتی، حسی و الگوی پروتئولیز عضله دو سر ران (Biceps femoris) شتر یک کوهانه بررسی شد. طول سارکومر، شاخص تجزیه میوفیبریل، نیروی برشی وارنر- ‌براتزلر، میزان کلاژن، آنالیز حسی و الگوی پروتئولیز پروتئین‌های میوفیبریلی با استفاده ازSDS-PAGE  مورد بررسی قرار گرفت. نتایج نشان داد با افزایش غلظت اسید و زمان نگهداری نمونه‌ها، میزان نیروی برشی و کلاژن کاهش پیدا کرد درحالی‌که شاخص تجزیه میوفیبریل و طول سارکومر افزایش یافت. همچنین نتایج حاصل از SDS-PAGE نشان داد که اثر زمان ‌بر مساحت باند (به‌عنوان شاخصی از پروتئولیز) زنجیره سنگین میوزین معنی‌دار نبود (05/0<p). درحالی‌که غلظت اسید اثر افزایشی معنی‌داری بر مساحت زنجیره سنگین داشت (05/0>p). در رابطه با پروتئین‌های c،α -اکتینین و اکتین هم غلظت و هم زمان اثر معنی‌دار و افزایشی در مساحت داشتند (05/0>p)، اما بر سایر باندها اثر معنی‌داری گزارش نشد (05/0<p). ارزیابی حسی نیز حاکی از تاثیر معنی‌دار ماریناد بر تردی و پذیرش کلی گوشت بود، به‌طوری که غلظت 5/1 درصد اسید بیشترین پذیرش و تردی را نشان داد (05/0>p). نتایج حاصل از این مطالعه نشان داد که ماریناد اسید لاکتیک می‌تواند به‌عنوان یک روش مؤثر بر ویژگی‌های بافتی و پروتئولیز عضله دوسر ران شتر به‌کار رود.

کلیدواژه‌ها

شتر؛ عضله دوسر ران؛ اسید لاکتیک

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