Evaluation of antioxidant potential and antimicrobial activity of Mocheh (Lepidium draba) extract “in vitro”

مجله پژوهشهای علوم و صنایع غذایی ایران

Article 2, Volume 17, Issue 3 - Serial Number 69, July and August 2021, Pages 17-24   

Document Type: Research Article-en

DOI: 10.22067/ifstrj.v18i1.86954

Authors

Sahar Roshanak¹; Behrooz Alizadeh Behbahani²; Fakhri Shahidi^{* 1}; Farideh Tabatabaei Yazdi¹; Alireza Vasiee¹; Neda Hashemi¹

¹Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

²Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

Abstract

Usage of natural products like herbals, provide unlimited opportunities for novel and suitable additives. Mocheh can be used in fresh form or as an ingredient in soup and salad. This study was aimed to determine the antimicrobial and antioxidant activities of Mocheh (Lepidium draba) extract. The antimicrobial activity of Mocheh extract was tested against Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Aspergillus niger. The extract showed a strong antimicrobial activity with a concentration dependence and a broad antimicrobial spectrum for all tested microorganism species. The results showed that MIC of leaf extract of Lepidium draba on Aspergillus niger, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus was 128, 128, 128 and 128 mg/mL respectively. The results showed that MBC/MFC of leaf extract of Mocheh on the examined microorganisms was 256, 256, 256 and 256 mg/mL respectively. The values obtained for total flavonoid content and total phenolic contents were 22.13 µg/mL and 18.88 mg/mL, respectively. Measured value in the radical scavenging activity was IC₅₀= 168/21 µL/mL. The results showed that Mocheh leaf aqueous extract is a novel source of natural antimicrobial and antioxidant agents for the food and pharmaceutical industries.

Keywords

Mocheh; Extract; Gram-positive and Gram-negative bacteria; Antioxidant activity

References

Al-Marzoqi, A., Al-Khafaji, K. R., & Kadhim, R. (2015). Influence of the crude Phenolic, Alkaloid and Terpenoid compounds extracts of Cardaria draba (Lepidium draba L.) on Human Pathogenic Bacteria. World Journal of Pharmaceutical Research 4(6), 456-460.

Amin Mir, M., Sawhney, S., & Manmohan, S. J. (2016). Antimicrobial Activity of Various Extracts of Taraxacum officinale. Journal of Microbial and Biochemical Technology, 8(3), 210-215.

Ardestani, A., & Yazdanparast, R. (2007). Antioxidant and free radical scavenging potential of Achillea santolina extracts. Food chemistry, 104(1), 21-29.

Baba, S. A., & Malik, S. A. (2015). Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4), 449-454.

Bazzaz, B. S., & Haririzadeh, G. (2003). Screening of Iranian Plants for Antimicrobial Activity. Pharmaceutical Biology, 41(8), 573-583.

Brantner, A., & Grein, E. (1994). Antibacterial activity of plant extracts used externally in traditional medicine. Journal of Ethnopharmacology, 44(1), 35-40.

Bursal, E., & Köksal, E. (2011). Evaluation of reducing power and radical scavenging activities of water and ethanol extracts from sumac (Rhus coriaria L.). Food Research International, 44(7), 2217-2221.

Capannesi, C., Palchetti, I., Mascini, M., & Parenti, A. (2000). Electrochemical sensor and biosensor for polyphenols detection in olive oils. Food Chemistry, 71(4), 553-562.

Chyad, A. (2017). Evaluation of anticancer, analgesic and anti-inflammatory activities of the ethanolic extract of Lepidium draba Linn. leaves. Adv. Anim. Vet. Sci, 5(1), 7-13.

Haghighi, M., & Mozafariyan, M. (2011). The introduction of extinct endemic vegetables of Iran. Journal of Medicinal Plants Research, 5(33), 7085-7107.

Hussein, H. M. (2016). Determination of phytochemical composition and ten elements content (CD, CA, CR, CO, FE, PB, MG, MN, NI AND ZN) of Cardaria Draba  by GC-MS, FT-IR and AAS techniques. International Journal of Pharma and Bio Sciences, 7(3), (B)1009-1017.

Klančnik, A., Piskernik, S., Jeršek, B., & Možina, S. S. (2010). Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. Journal of Microbiological Methods, 81(2), 121-126.

Kossah, R., Zhang, H., & Chen, W. (2011). Antimicrobial and antioxidant activities of Chinese sumac (Rhus typhina L.) fruit extract. Food Control, 22(1), 128-132.

Kuete, V. (2010). Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Medica, 76(14), 1479-1491.

Kuete, V., & Efferth, T. (2010). Cameroonian medicinal plants: pharmacology and derived natural products. Frontiers in pharmacology, 1, 123-132.

Radonić, A., Blažević, I., Mastelić, J., Zekić, M., Skočibušić, M., & Maravić, A. (2011). Phytochemical analysis and antimicrobial activity of Cardaria draba (L.) Desv. volatiles. Chemistry & biodiversity, 8(6), 1170-1181.

Shan, B., Cai, Y.-Z., Brooks, J. D., & Corke, H. (2007). The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of Food Microbiology, 117(1), 112-119.

Sigstedt, S. C., Hooten, C. J., Callewaert, M. C., Jenkins, A. R., Romero, A. E., Pullin, M. J., Kornienko, A., Lowrey, T. K., Slambrouck, S. V., & Steelant, W. F. (2008). Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells. International journal of oncology, 32(5), 1085-1090.

Sohail, M., Iqbal, Z., Afzal, M., Afzal, A., Ur Rahman, I., & Shad, S. (2014). In vitro antibacterial study of Taraxacum officinale leaves extracts against different bacterial pathogenic strains. Journal of Pharmacognosy and Phytochemistry, 3(2), 15-17.

Tchinda, C. F., Voukeng, I. K., Beng, V. P., & Kuete, V. (2017). Antibacterial activities of the methanol extracts of Albizia adianthifolia, Alchornea laxiflora, Laportea ovalifolia and three other Cameroonian plants against multi-drug resistant Gram-negative bacteria. Saudi Journal of Biological Sciences, 24(4), 950-955.

Wendakoon, C., Calderon, P., & Gagnon, D. (2012). Evaluation of selected medicinal plants extracted in different ethanol concentrations for antibacterial activity against human pathogens. Journal of Medicinally Active Plants, 1(2), 60-68.