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داوری نژاد, غلامحسین, کریم پور, ساجده. (1400). تولید گیاهان خودریشه گلابی رقم ʼنطنزʽ (Pyrus communis L. cv. Natanz) در شرایط درون‌شیشه‌ای. سامانه مدیریت نشریات علمی, 35(3), 341-354. doi: 10.22067/jhs.2021.60797.0
غلامحسین داوری نژاد; ساجده کریم پور. "تولید گیاهان خودریشه گلابی رقم ʼنطنزʽ (Pyrus communis L. cv. Natanz) در شرایط درون‌شیشه‌ای". سامانه مدیریت نشریات علمی, 35, 3, 1400, 341-354. doi: 10.22067/jhs.2021.60797.0
داوری نژاد, غلامحسین, کریم پور, ساجده. (1400). 'تولید گیاهان خودریشه گلابی رقم ʼنطنزʽ (Pyrus communis L. cv. Natanz) در شرایط درون‌شیشه‌ای', سامانه مدیریت نشریات علمی, 35(3), pp. 341-354. doi: 10.22067/jhs.2021.60797.0
داوری نژاد, غلامحسین, کریم پور, ساجده. تولید گیاهان خودریشه گلابی رقم ʼنطنزʽ (Pyrus communis L. cv. Natanz) در شرایط درون‌شیشه‌ای. سامانه مدیریت نشریات علمی, 1400; 35(3): 341-354. doi: 10.22067/jhs.2021.60797.0

تولید گیاهان خودریشه گلابی رقم ʼنطنزʽ (Pyrus communis L. cv. Natanz) در شرایط درون‌شیشه‌ای

علوم باغبانی
مقاله 3، دوره 35، شماره 3 - شماره پیاپی 51، آذر 1400، صفحه 341-354    اصل مقاله (683.21 K)
نوع مقاله: مقالات پژوهشی
شناسه دیجیتال (DOI): 10.22067/jhs.2021.60797.0
نویسندگان
غلامحسین داوری نژاد* ؛ ساجده کریم پور
گروه علوم باغبانی و مهندسی فضای سبز، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
چکیده
به منظور تولید درختان خودریشه گلابی رقم ʼنطنزʽ (Pyrus communis cv. Natanz) در شرایط درون شیشه‌ای سه آزمایش جداگانه برای بهبود پرآوری شاخه (تاثیرات BAP و Fe-NaEDDHA)، استقرار مریستم (تاثیرات BAP و GA3) و ریشه‌دهی ریزشاخه‌ها (تاثیرات IBA و NAA) در قالب طرح کاملاً تصادفی به اجرا در آمد. شاخه‌ها در محیط کشت PMI (MS ×1.5 CaCl2. 2H2O, KH2PO4 and MgSO4. 7H2O) حاوی 5/1 میلی‌گرم بر لیتر BAP و صفر میلی‌گرم بر لیتر Fe-NaEDDHA پرآوری شدند (50/5 شاخه/ریزنمونه) و رشد طولی خوبی داشتند در صورتی‌که غلظت‌های پایین BAP و Fe-NaEDDHA برگ‌های بالغ بیشتری را تولید کرد. محیط کشت PMI غنی شده با 1 میلی‌گرم بر لیتر BAP بعلاوه 150 میلی‌گرم بر لیتر Fe-NaEDDHA برای پرآوری شاخه نطنز بدلیل بالاترین میزان در رشد رویشی و بالاترین کیفیت شاخه‌های پرآوری شده پیشنهاد می‌شود. محیط کشت MS حاوی 5/0 میلی‌گرم بر لیتر BAP و 5/0 میلی‌گرم بر لیتر GA3 و یا 1 میلی‌گرم بر لیتر BAP (81 درصد) و 1/0 میلی‌گرم بر لیتر GA3 (63 درصد) به ترتیب بالاترین درصد استقرار مریستم را داشتند. ریزشاخه‌های درون شیشه‌ای نطنز از طریق فروبری سریع در محلول IBA+NAA (1000+1000 میلی‌گرم بر لیتر) و سپس قرار دادن در محیط کشت PMI ریشه‌دار شدند.
کلیدواژه‌ها
ریزازدیادی؛ ریشه‌دهی؛ کشت مریستم؛ Fe-NaEDDHA
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