Evaluation of Fruit Quantitative and Qualitative Characteristics of some Bene Genotypes (Pistacia atlantica) at Green Stage

علوم باغبانی

Article 10, Volume 35, Issue 3 - Serial Number 51, June 2021, Pages 447-458   

Document Type: Research Article

DOI: 10.22067/jhs.2021.67091.0

Authors

Salahedin Mslahate fard^{* 1}; Hamid Hassanpour²

¹M.Sc. Graduate in Horticultural Sciences Department of Horticulture, Urmia University, Urmia

²Associate Professor, Department of Horticulture, Urmia University, Urmia

Abstract

Introduction: The pistachio genus (Pistacia) belongs to Anacardiaceae family. Three species of common pistachio (P. vera, P. khinjuk and P. atlantica) was found in Iran. Bene )P. atlantica (is a dicotyledonous tree with a height of more than 17 meters, is the most important species of Zagros region with valuable main and secondary products. This tree was also used as a rootstocks for edible pistachios and resistant to adverse environmental conditions, so that it grows in different climates in terms of land slope, soil type, different soil fertility levels, altitude (600 to 3000 meters) and temperature range of -25 to 40 °C.  Fruit and gum have different uses in food, industry and medicine. Medicinal and oral uses of Bene fruit and gum have long been reported not only in Iran, but also among the natives of all coriander-rich regions, including parts of Algeria, Turkey and Iraq. Bene fruit can be harvested at three stages; First, the unripe stage (in the form of small hollow spheres without a kernel), its skin is brittle and its color is pale green. The second stage, the kernel is completely hardened, but the shell has an ocher red color that tastes sour. The last, the ripe stage, the kernel is completely hardened and the color of the skin is blue-green.
Materials and Methods: This study was conducted to evaluate the diversity and determine some quantitative and qualitative characteristics of different Bene) P. atlantica (genotypes at the green fruit stage. For this purpose, 11 genotypes were randomly selected in an area near the city of Mirabad, West Azerbaijan Province, Iran. The fruits were harvested from each genotype and transferred to the laboratory. In this study, some quantitative traits of fruit and cluster as well as total phenol, total flavonoids and total antioxidant capacity were analyzed. Digital calipers were used to measure the length and width of fruit and clusters (mm). Also, a digital scale with an accuracy of 0.001 g was used to measure the weight of fruit and cluster. Fruit size index was calculated by multiplying the measured width by the measured length and expressed as mm². Total antioxidant capacity was assessed using the DPPH (1, 1-diphenyl-2-picrylhydrazyl) method. Folin–Ciocalteu method was used to measure the total phenol content.
Results and Discussion: The results showed that cluster weight had the highest diversity and fruit width had the least diversity among the studied traits. Traits such as cluster width, cluster length, total phenol and flavonoid content were also highly varied among genotypes. In this study, the maximum weight, length and width of fruit were 1.28 g, 8.32 and 8.62 mm, respectively. Also, the results of this study showed that there is a significant difference between genotypes in terms of all parameters measured except cluster length at the 1% and 5% of probability level. Overall, the results of this study showed high variability in the majority of traits. The highest length, width and weight of the cluster as well as the width and weight of the fruit were seen in P10 genotype and the lowest was observed in P5 and P6 genotypes. In terms of biochemical characteristics, P3 genotype had the highest total phenol (1034.5 mg gallic acid per gram of FW), total flavonoid (143.82 mg quercetin per gram FW) and antioxidant capacity (93%) and also, the lowest total phenol (370.08 mg gallic acid per gram of FW), total flavonoids (62.25 mg catechin per gram FW) was observed in P7 genotype. According to results of this study, P3 and P10 genotypes can be used for medicinal, food, industrial purposes as well as breeding purposes compared to the other genotypes.
Conclusion: In this study, it was shown that there is a high diversity in the majority of measured traits among different genotypes. The highest morphological traits were observed in P10 genotype. Also, in terms of biochemical characteristics, genotype P3 had the highest total phenol, total flavonoid content and total antioxidant capacity. According to the results, it can be concluded that the morphological characteristics as well as phenolic, flavonoid compounds and antioxidant capacity depend on various factors, including genetic factors and environmental and ecological conditions. Therefore, the observed diversity can be related to genetic structure of genotypes, which can also be considered as a kind of adaptation and physiological response of trees to environmental conditions.

Keywords

Antioxidant activity; Flavonoid; Fruit weight; Total phenol

References

• Akbari V., Jamei R., Heidari R., and Jahanban Sfahlan A. 2012. Antioxidant activity of different parts of Walnut (Juglans regia) Fruit as a function of genotype. Food Chemistry 135: 2404–2410.
• Ariza M.T., Reboredo-Rodríguez P., Cervantes L., Soria C., Martínez-Ferri E., González-Barreiro C., Cancho-Grande B., Battino M., and Simal-Gándara J. 2018. Bioaccessibility and potential bioavailability of phenolic compounds from achenes as a new target for strawberry breeding programs. Food Chemistry 248: 155-165.
• Bahrebar M., Mirzaei A., Mantegheyan E., and Bahrebar A. 2012. In vivo and in vitro antioxidant activity of hydro-alcoholic extract of Pistacia atlantica. Armaghane Danesh 17(6): 540-551. (In Persian with English abstract)
• Baqerzadeh Gh., and Nakhaee M. 2015. Quantitative and qualitative study of physical and chemical, phytochemical and antioxidant effects of pistachio leaf (Pistacia atlantica), native to Birjand city. Journal of Plant Research (Iranian Journal of Biology) 30(2): 273-279. (In Persian with English abstract)
• Benamar H., Rached W., Derdour A., and Marouf A. 2010. Screening of Algerian medicinal plants for acetylcholinesterase inhibitory activity. Journal of Biological Sciences 10(1): 1-9.
• Benhassaini H., Bendahmane M., and Benchalgo N. 2007. The chemical composition of fruits of Pistacia atlantica Subsp. Atlantica from Algeria. Chemistry of Natural Compounds 43(2): 121.
• Farhoosh R., and Tavakoli J. 2008. Physicochemical properties of kernel oil from Amygdalus scoparia growing wild in Iran. Journal of Food Lipids 15(4): 433-443.
• Ganji F., Bashtani M., Farhang Far H., and Ghiasi S. 2017. Alteration of antioxidant properties, nutritional value and rumen-intestinal digestibility of pistachio fruit (Pistacia atlantica) by nylon bags. Iranian Journal of Animal Science Research 27(3): 185-200. (In Persian with English abstract)
• Ganji F., Bashtani M., Farhang Far H., and Ghiasi S. 2018. Alteration of antioxidant properties, nutritional value and rumen-intestinal digestibility of pistachio leaf (Pistacia atlantica) by nylon bags. Iranian Journal of Animal Science Research 10(4): 477-488. (In Persian with English abstract)
• Ghasemi Pirbalouti A., and Aghaee K. 2011. Chemical composition of essential oil of Pistacia khinjuk stocks grown in Bakhtiari Zagross Mountains, Iran. Electronic Journal of Biology 7(4): 67–69.
• Gholami Sh., Hosseini S., and Sayad A. 2007. Effect of weeding, depth and time of sowing seeds on the growth of coriander seedlings in nurseries. Journal of Research and Construction 75: 71-80. (In Persian with English abstract)
• Hassanpour H., and Alizadeh S. 2016. Evaluation of phenolic compound, antioxidant activities and antioxidant enzymes of barberry genotypes in Iran. Scientia Horticulturae 200: 125–130.
• Hatamnia A.A., Abbaspour N., and Darvishzadeh R. 2014. Antioxidant activity and phenolic profile of different parts of Bene (Pistacia atlantica Kurdica) fruits. Food chemistry 145: 306-311.
• Hatamnia A., Malekzadeh P., Noorullahi Kh., and Valad Beigi T. 2015. Study of phenolic compounds content and antioxidant activity of Pistacia khinjuk Stocks in natural habitats of Ilam province. Journal of Plant Environmental Physiology 10(40): 31-40. (In Persian with English abstract)
• Kafkas S., and Prel-Treves R. 2002. Morphological and molecular phylogeny of Pistacia species in Turkey. Theoretical and Applied Genetics 102: 908-910.
• Labdelli A., Rebiai A., Tahirine M., Adda A., and Merah O. 2020. Nutritional content and antioxidant capacity of the seed and the epicarp in different ecotypes of Pistacia atlantica Subsp. Atlantica. Plants 9: 1065.
• Malekzadeh P., Hatamnia A.A.H., and Nourollahi K. 2015. Total phenolic content and antioxidant activity of fruit and leaf of Bene (Pistacia atlantica Kurdica) in Ilam province. Plant Physiology 6(1): 1543-1549.
• Mirzaei A., Mohammadi J., Mirzaei N., and Mirzaei M. 2011. The antioxidant Capacities and total phenolic contents of some medicinal plants in Iran. Journal of Fasa University of Medicinal Sciences 1(3): 160-166. (In Persian with English abstract)
• Mozaffarian V. 2004. Trees and shrubs of Iran. Contemporary Culture Publications, Tehran, 990 pages.
• Nadjat T., and Tayeb T. 2020. Effect of environmental conditions on morphological variability of leaves and fruits of five populations of Pistacia atlantica in North Algeria. Biodiversity Research and Conservation 58: 1-12.
• Pourreza M., Shaw J.D., and Zangeneh H. 2008. Sustainability of wild pistachio (Pistacia atlantica) In Zagros forests, Iran. Forest Ecology and Management 255(11): 3667-3671.
• Rigane G., Ghazghazi H., Aouadhi C., Ben Salem R., and Nasr Z. 2017. Phenolic content, antioxidant capacity and antimicrobial activity of leaf extracts from Pistacia atlantica. Natural Product Research 31(6): 696-699.
• Rostami Kia Y., Fattahi M., and Faith P. 2009. Investigation of genetic diversity of pistachio populations using leaf and fruit morphological traits. Two Quarterly Journal of Genetic Research and Range and Plant Breeding of Iran 17(2): 284-294. (In Persian with English abstract)
• Sarwari A., Dianti Tilki Gh., Rezaei M.B., and Zadbar M. 2015. The effect of some environmental factors on the quantity and quality of plant essential oil Stachys lavandifolia vahl in Khorasan Razavi province (Chenaran). Quarterly Journal of Medicinal Plants Ecophytochemistry 2(3): 1-7. (In Persian with English abstract)
• Seyedi N., jalali S.G.A., Moghaddam M., Tabari M., and Mohammadi S.A. 2011. Application of seed storage protein in inter-specific variation in three population of pistacia atlantica Iranian Journal of Plant Biology 2(6): 1-13. (In Persian with English abstract)
• Shin S.W., Ghimeray A.K., and Park C.H. 2014. Investigation of total phenolic, total flavonoid, antioxidant and allyl isothiocyanate content in the different organs of wasabi japonica grown in an organic system. African Journal of Traditional Complementary Alternative Medicines 3(11): 38-45.
• Suleiman Beigi M., and Arzegar Z. 2013. A review study on chemical properties and food index of mastic oil compared with olive, sunflower and canola oils. The ilamian traditional uses of mastic. Scientific Journal of Ilam University of Medical Sciences 21(5): 1-13. (In Persian with English abstract)
• Tabandeh Sarvai A., and Nadi H. 2018. Effect of altitude and genotype on morphological diversity of pistachio leaves (Pistacia atlantica Mutica) in the forests of southern Yazd province. Dry Canvas Research 2(8): 16-25. (In Persian with English abstract)
• Wang J., Wang J., Ye J., Vanga S.K., and Raghavan V. 2019. Influence of high-intensity ultrasound on bioactive compounds of strawberry juice: Profiles of ascorbic acid, phenolics, antioxidant activity and microstructure. Food Control 96: 128-136.
• Yousefi B. 2002. A comparative study of ecological and genetic characteristics of Torbatin tree population in Kurdistan province. Iranian Journal of Forest and Poplar Research 266(7): 65-99. (In Persian with English abstract)
• Yousefi B. 2015. Comparison of morphological and chemical properties of pistachio fruit (Pistacia atlantica) in two model habitats of Kurdistan province. Iranian Journal of Forest and Poplar Research 23(2): 368-378. (In Persian with English abstract)