تأثیر پوترسین و نیتریک‌اکسید بر بهبود ویژگی‌های مورفولوژیکی، بیوشیمیایی و پس‌از‌برداشت رز رقم ’آوالانچ‘

علوم باغبانی

مقاله 14، دوره 36، شماره 1 - شماره پیاپی 53، خرداد 1401، صفحه 193-212   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jhs.2021.69604.1036

نویسندگان

رقیه عبدی؛ زهره جبارزاده^*

گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

چکیده

گل رز (Rosa hybrida ‘Avalanche’) یکی از گل­های بریدنی­ مهم دنیاست. به­منظور بررسی تاثیر محلول‌پاشی سدیم نیتروپروساید (به­عنوان منبع نیتریک­اکسید) و پوترسین بر گل رز رقم ’آوالانچ‘ پژوهشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در شرایط کشت هیدروپونیک به صورت گلدانی در گلخانه اجرا شد. تیمارهای آزمایش شامل چهار غلظت سدیم نیتروپروساید (صفر، 50، 100 و 200 میکرومولار) و چهار غلظت پوترسین (صفر، 1، 2 و 4 میلی­مولار) بود. شاخص­های اندازه­گیری شده شامل وزن تر و خشک ساقه گل­دهنده، کلروفیل  b ,aو کلروفیل کل، کاروتنوئید و در مرحله پس از برداشت آنزیم­های گایاکول پراکسیداز و آسکوربات پراکسیداز و خمیدگی ساقه بودند. سدیم نیتروپروساید در غلظت 50 میکرومولار همراه با پوترسین 4 میلی­مولار، وزن تر و خشک ساقه گلدهنده را نسبت به شاهد افزایش داد. غلظت 100 میکرو مولار سدیم نیتروپروساید همراه با کاربرد ۴ میلی‌مولار پوترسین باعث افزایش معنی­دار میزان کلروفیل  b ,aو کلروفیل کل و کاروتنوئید نسبت به شاهد شد. لازم به ذکر است که کاربرد قبل از برداشت سدیم­نیتروپروساید و پوترسین در بهبود ویژگی­های پس از برداشت گل رز نیز تاثیر مثبت داشت، به­طوری که بیشترین میزان فعالیت آنزیم­های آنتی­اکسیدانی (آسکوربات پراکسیداز و گایاکول پراکسیداز) و کمترین خمیدگی ساقه در غلظت­های 100 و 200 میکرومولار سدیم نیتروپروساید به تنهایی و یا همراه با کاربرد پوترسین مشاهده شد. به­طور کلی، سدیم­نیتروپروساید و پوترسین تاثیر مثبت و مطلوبی در بهبود شاخص­های رشدی و پس از برداشتی گل رز داشتند ولی غلظت موثر بسته به نوع شاخص متفاوت بود.

کلیدواژه‌ها

آنزیم‌های آنتی‌اکسیدانی؛ پلی‌آمین؛ خمیدگی ساقه؛ سدیم‌نیتروپروساید

مراجع

1. Abdel Aziz N.G., Taha Lobna S., and Ibrahim Soad M.M. Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituent of Gladiolous plants at Nuberia. Ozean. Journal of Applied Sciences 2(2): 169-179.
2. Arab S., Baradaran Firoz Abadi M., Asghari A., and Rahimi M. The effect of foliar application of ascorbic acid and sodium nitroprusside on grain protein content, yield and some agronomic traits of safflower under water deficit stress. Journal of Crop Production 38(4): 93-104.
3. Asghari M. Hormones and New (Non-Classic) Plants Growth Regulators. Urmia University press. (In Persian)
4. Asna Ashari M., and Zokai Khosroshahi M. Polyamines and Horticultural Sciences. Bu-Ali Sina University, 163 p. (In Persian)
5. Ataii D., Khandan-Mirkohi A., and Naderi R. Exogenous putrescine delays senescence of Lisianthus cut flowers. Journal of Ornamental Plants 3: 167-174.
6. Badawy E.M., Kandil M.M., Mahgoub M., Shanan N., and Hegazi N. Chemical constituents of Celosia argentea var. cristata L. plants as affected by foliar application of putrescine and alpha-tocopherol. International Journal of ChemTech Research 8(12): 464-470.
7. Bani Assadi F., Safari V.R., and Maghsoudi A.A. 2015. Effect of putrescine and salinity on the morphological, biochemical, and pigments of English marigold plant (Calendula officinalis). Journal of Science and Technology of Greenhouse Cultures 6(21): 125–133. (In Persian with English abstract)
8. Bhattacharjee S.K., and Banerji B.K. 2010. The Complete Book of Roses. Jaipur 302 003 (Raj.) India. 531 p.
9. Chen D., Shao Q., Yin L., Younis A., and Zheng B. 2019. Polyamine function in plants: metabolism, regulation on development, and roles in abiotic stress responses. Frontiers in Plant Science 9: 1945. https://doi.org/10.3389/fpls.2018.01945.
10. ‏Danaee E., and Abdossi V. 2018. Effect of different concentration and application methods of polyamines (Putrescine, Spermine, Spermidine) on some morphological, physiological, and enzymatic characteristics and vase life of Rosa hybrida Dolce Vita cut flower. Journal of Ornamental Plants 8(3): 171-182.
11. Dastyaran M., and Hosseini Farahi M. 2014. The effect of humic acid and putrescin on vegetative properties and vase life of rose in a soilless system. Journal of Science and Technology of Greenhouse Cultures 20: 243–252. (In Persian with English abstract)
12. Deng Y., Wang C., Huo J., Hu W., and Liao W. 2019. The involvement of NO in ABA-delayed the senescence of cut roses by maintaining water content and antioxidant enzymes activity. Scientia Horticulturae 247: 35-41. https://doi.org/10.1016/j.scienta.2018.12.006.
13. Duan X., Su X., You Y., Qu H., Li Y., and Jiang Y. 2007. Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism. Food Chemistry 104(2): 571-576. https://doi.org/10.1016/j.foodchem.2006.12.007.
14. Eslami M., Nasibi F., Manouchehri Kalantari K., Khezri M., and Oloumi H. 2019. Effect of exogenous application of L-arginine and sodium nitroprusside on fruit abscission and physiological disorders of pistachio (Pistacia vera) Scions. International Journal of Horticultural Science and Technology 6(1): 51-62.
15. Fan H.F., Du C.X., Ding L., and Xu Y.L. 2014. Exogenous nitric oxide promotes waterlogging tolerance as related to the activities of antioxidant enzymes in cucumber seedlings. Russian Journal of Plant Physiology 61(3): 366-373. https://doi.org/1134/S1021443714030042.
16. Ferrante A., Alberici A., Antonacci S., and Serra G. 2007. Effect of promoter and inhibitors of phenylalanine ammonia-lyase enzyme on stem bending of cut gerbera flowers. In International Conference on Quality Management in Supply Chains of Ornamental 755: 471-476.
17. Fraser P.D., and Bramley P.M. 2004. The biosynthesis and nutritional uses of carotenoids. Progress in Lipid Research 43(3): 228-265. https://doi.org/10.1016/j.plipres.2003.10.002.
18. Gohari G., Alavi Z., Esfandiari E., Panahirad S., Hajihoseinlou S., and Fotopoulos V. 2020. Interaction between hydrogen peroxide and sodium nitroprusside following chemical priming of Ocimum basilicum against salt stress. Physiologia Plantarum 168(2): 361-373.‏ https://doi.org/10.1111/ppl.13020.
19. Gomez-Ros L.V., Gabaldon C., Nunez-Flores M.J.L., Gutierrez J., Herrero J., Zapata J.M., Sottomayor M., Cuello J., and Barcelo A.R. 2012. The promoter region of the Zinnia elegans basic peroxidase isoenzyme gene contains cis-elements responsive to nitric oxide and hydrogen peroxide. Planta 236(2): 327-342. https://doi.org/1007/s00425-012-1604-3. 
20. Gupta D.K., Palma J.M., and Corpas F.J. 2019. Nitric Oxide and Hydrogen Peroxide Signaling in Higher Plants. Springer, 275p.
21. Hemati E., Daneshvar M.H., and Heidari M. 2019. The roles of sodium nitroprusside, salicylic acid, and methyl jasmonate as hold solutions on vase life of Gerbera jamesonii ‘Sun Spot’. Advances in Horticultural Science 33(2): 187-195. https://doi.org/10.13128/ahs-24261.
22. Hosseini Farahi M., Khalighi A., Kholdbarin B., Mashhadi Akbar-Boojar M., and Eshghi S. 2013. Morphological responses and vase life of Rosa hybrida Dolce Vita to polyamines spray in hydroponic system. World Applied Sciences Journal 21(11): 1681-1686. https://doi.org/10.5829/idosi.wasj.2013.21.11.1868.
23. Hosseini H., and Rezaei Nezhad A. 2016. Effects of foliar application of sodium nitroprusside on drought tolerance of Marigold. Iranian Journal of Horticulture Science and Technology 17(3): 285-298. (In Persian with English abstract)
24. Jabbarzadeh M., Tehranifar A., Amiri J., and Abedi B. 2016. Investigation on the protective role of nitric oxide in reducing damages induced by salinity stress in Calendula officinalis Journal of Horticultural Science 30(2): 185-191. (In Persian with English abstract)
25. Jalili Marandi R. 2010. Physiology of Environmental Stresses and Resistance Mechanisms in Garden Plants. Jahad Daneshghahi of Urmia press, 636P. (In Persian)
26. Kahrobaiyan M., Nemati S.H., Rahemi M., Kholdebarin B., and Tehranifar A. 2019. Morphological responses of ornamental sunflower to putrescine treatment under drought conditions. Applied Ecology and Environmental Research 17(3): 6117-6127.
27. Kakkar, R.K., and Sawhney, V.K. 2003. Polyamine research in plants- a changing perspective. Physiolgia Plantarium 116:281-292. https://doi.org/1034/J.1399-3054.2002.1160302.X.
28. Kamiab F., and Zamani Bahramabadi E. 2016. The effect of different polyamines on some physiological traits as ACC oxidase and superoxide dismutase enzymes activity in Chrysanthemum morifolium Bright Golden Ann. Journal of Ornamental Plants 6(2): 83-91.
29. Kandil M.M., Soad M.M., Lbrahaim S.H., El-Hanafy S.H., and El-Sabwah M.M. 2015. Effect of putrescine and uniconazole on some flowering characteristics and some chemical constituents of Saliva splendens plant. Journal of Chemical Technology and Biotechnology 8(9): 178-186. https://doi.org/10.21608/ejarc.2012.214880.
30. Kang H.M., and Saltveit M.E. 2002. Chilling tolerance of maize, cucumber and rice seedling leaves and roots and differentially affected by salicylic acid. Plant Physiology 115: 571-576. https://doi.org/10.1034/j.1399-3054.2002.1150411.x.
31. Karamiyan R. 2019. Study of the effects of salicylic acid, sodium nitroprusside and ethanol on vase life and flower quality of cut flowers of two gerbera varieties. Journal of Plant Research 32(3): 635-646.
32. Kazemzadeh-Beneh H., Samsampour D., and Zarbakhsh S. 2018. Biochemical, physiological changes and antioxidant responses of cut gladiolus flower 'White Prosperity' induced by nitric oxide. Advances in Horticultural Science 32(3): 421-431. https://doi.org/10.13128/ahs-23361.
33. Khosh-Khui M., Sheybani B., Rohani E., and Tafazoli A. 2010. Principles of Horticulture. Shiraz University press. (In Persian)
34. Kumar S., Yadav P., Jain V., and Malhotra S.P. 2014. Isozymes of antioxidative enzymes during ripening and storage of ber (Ziziphus mauritiana ). Food Science and Technology 51: 329-334. https://doi.org/10.1007/s13197-011-0489-7.
35. Li X., Yang Y., Yao J., Chen G., Li X., Zhang Q., and Wu C. 2009. Flexible culm encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice. Plant Molecular Biology 69(6): 685-697. https://doi.org/10.1007/s11103-008-9448-8.
36. Lichtenthaler H.K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350-382. https://doi.org/10.1016/0076-6879(87)48036-1.
37. Mahgoub M.H., El-Aziz N.A., and Mazhar A.M.A. 2011. Response of Dahlia pinnata plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American-Eurasian Journal of Agricultural and Environmental Sciences 10(5): 769-775.
38. Mohammadi H., Ghorbanpour M., and Brestic M. 2018. Exogenous putrescine changes redox regulations and essential oil constituents in field-grown Thymus vulgaris under well-watered and drought stress conditions. Industrial Crops and Products 122: 119-132. https://doi.org/10.1016/j.indcrop.2018.05.064.
39. Monzon G.C., Pinedo M., Di Rienzo J., Novo-Uzal E., Pomar F., Lamattina L., and de la Canal L. 2014. Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses. Nitric Oxide 39: 20-28. https://doi.org/1016/j.niox.2014.04.004.
40. Mostafaei E., Zehtab-Salmasi S., Salehi-Lisar Y., and Ghassemi-Golezani K. 2018. Changes in photosynthetic pigments, osmolytes and antioxidants of Indian Mustard by drought and exogenous polyamines. Acta Biologica Hungarica 69(3): 313-324. https://doi.org/1556/018.68.2018.3.7.
41. Mostofi Y., Rasouli P., Naderi R., Bagheri Marandi G., and Shafiei M. 2010. Effect of nitric oxide and thidiazuron on vase life and some qualitative characteristics of cut carnation flower (Dianthus caryophyllus Nelson). Iranin Journal of Horticulture Science 41: 301-308. (In Persian with English abstract)
42. Nakano Y., and Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Journal of Plant Cell Physiology 22: 867-880. https://doi.org/10.1093/oxfordjournals.pcp.a076232.
43. Neily M.H., Matsukura C., Maucourt M., Bernillon S., Deborde C., Moing A., Yin Y., Saito T., Mori K., Asamizu E., Rolin D., Moriguchi T., and Ezura H. 2011. Enhanced polyamine accumulation alters carotenoid metabolism at the transcriptional level in tomato fruit over-expressing spermidine synthase. Journal of Plant Physiology 168: 242-252. https://doi.org/1016/j.jplph.2010.07.003.
44. Perik R.R., Razé D., Harkema H., Zhong Y., and Van Doorn W.G. 2012. Bending in cut Gerbera jamesonii flowers relates to adverse water relations and lack of stem sclerenchyma development, not to expansion of the stem central cavity or stem elongation. Postharvest Biology and Technology 74: 11-18. https://doi.org/10.1016/j.postharvbio.2012.06.009.
45. Pirzad A.R., and Mohammadzadeh S. 2018. Crop Physiology. Urmia University Press. (In Persian)
46. Salachna P., and Zawadzińska A. 2018. Effect of nitric oxide on growth, flowering and bulb yield of Eucomis autumnalis. In VII International Conference on Managing Quality in Chains (MQUIC2017) and II International Symposium on Ornamentals in 1201: 635-640.
47. Sadeghi J., Farahmand H., Nasibi F., and Hosseini F. 2016. Effect of nitric oxide on physiological and antioxidant responses and reducing stem neck of gerbera cut flower. Iranian Journal of Horticultural Sciences and Technologies 17(2): 193-208. (In Persian with English abstract)
48. Salachna P., Zawadzińska A., Wierzbiński Ł., and Senderek W. 2016. Enhancing growth in Eucomis autumnalis (Mill.) Chitt. seedlings with exogenous application of nitric oxide. Journal of Horticultural Research 24(2): 13-17.
49. Sami F., Faizan M., Faraz A., Siddiqui H., Yusuf M., and Hayat S. 2017. Nitric oxide-mediated integrative alterations in plant metabolism to confer abiotic stress tolerance, NO crosstalk with phytohormones and NO-mediated post translational modifications in modulating diverse plant stress. Nitric Oxide 73: 22-38. https://doi.org/1016/j.niox.2017.12.005.
50. Shabanian, S., Esfahani, M.N., Karamian, R., and Tran, L.S.P. 2018. Physiological and biochemical modifications by postharvest treatment with sodium nitroprusside extend vase life of cut flowers of two gerbera cultivars. Postharvest Biology and Technology 137: 1-8. https://doi.org/10.1016/j.postharvbio.2017.11.009.
51. Shi H., Liu W., Wei Y., and Ye T. 2017. Integration of auxin/indole-3-acetic acid 17 and RGA-LIKE3 confers salt stress resistance through stabilization by nitric oxide in Arabidopsis. Journal of Experimental Botany 68: 1239–1249. https://doi.org/1093/jxb/erw508.
52. Singh S., Kumar V., Kapoor D., Kumar S., Singh S., Dhanjal D.S., and Singh J. 2020. Revealing on hydrogen sulfide and nitric oxide signals co‐ordination for plant growth under stress conditions. Physiologia Plantarum 168(2): 301-317.‏ https://doi.org/1111/ppl.13002.
53. Talebi F. 2011. The effects of nitric oxide and thidiazuron on qualitative traits and shelf life of cut roses (Rosa hybrida Sensiro) (M.Sc. Thesis). Zanjan University. (In Persian)
54. Upadhyaya A., Sankhla D., Davis T.D., Sankhla N., and Smith B.N. 1985. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. Journal of Plant Physiology 121(5): 453-461. https://doi.org/10.1016/S0176-1617(85)80081-X.
55. Vuosku J., Karppinen K., Muilu-Mäkelä R., Kusano T., Sagor G.H.M., and Avia K. 2018. Scot’s pine aminopropyl transferases shed new light on evolution of the polyamine biosynthesis pathway in seed plants. Annals of Botany 121: 1243–1256. https://doi.org/10.1093/aob/mcy012.
56. Wang M., Li B., Zhu Y.C., Niu L.J., Jin X., Xu Q.Q., and Liao W.B. 2015. Effect of exogenous nitric oxide on vegetative and reproductive growth of oriental lily ‘Siberia’. Horticulture, Environment and Biotechnology 56(5): 677-686. https://doi.org/1007/s13580-015-0051-z.
57. Yousefi F., Jabbarzadeh Z., Amiri J., and Rasouli-Sadaghiani M.H. 2019. Response of Roses (Rosa hybrida ‘Herbert Stevens’) to foliar application of polyamines on root development, flowering, photosynthetic pigments, antioxidant enzymes activity and NPK. Scientific Reports 9(16025): 1-11. 025. https://doi.org/10.1038/s41598-019-52547-1.
58. Zeng C.L., Liu L., and Xu G.Q. 2011. The physiological responses of carnation cut flowers to exogenous nitric oxide. Scientia Horticulturae 127(3): 424-430. https://doi.org/10.1016/j.scienta.2010.10.024.
59. Zhou R., Li Y., Yan L., and Xie J. 2011. Effect of edible coatings on enzymes, cell membrane integrity, and cell-wall constituents in relation to brittleness and firmness of Huanghua pears (Pyrus pyrifolia Huanghua) during storage. Food Chemistry 124: 569-575. https://doi.org/10.1016/j.foodchem.2010.06.075.