A Multi-Faceted Approach for Prediction of Genome Safe Harbor Loci in the Chicken Genome

Journal of Cell and Molecular Research

مقاله 1، دوره 13، شماره 1 - شماره پیاپی 25، آذر 2021، صفحه 1-9  

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2021.71604.1015

نویسندگان

Nima Dehdilani^{1، 2}؛ Mohsen Fathi Najafi³؛ Hesam Dehghani ^{1، 2، 4}

¹Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

²Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Iran

³Education and Extension Organization (AREEO), Agricultural Research, Razi Vaccine and Serum Research Institute, Mashhad Branch, Mashhad, Iran

⁴Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

To achieve a reliable and persistent expression, the transgene should be precisely integrated into the genome safe harbor (GSH) loci. Little attention has been paid to find the safe harbor loci of the chicken (Gallus gallus domesticus) genome. Identification and characterization of GSH loci that allow the persistent and reliable expression of knock-in genes could be a major area of interest within the field of transgenic technology and is central to the development of transgenic livestock. Randomly integrated transgenes might encounter position effects and epigenetic silenc­ing, so unstable phenotypes, as well as unreliable and unpredictable expression of the knock-in transgene could occur. In contrast to random gene insertion, site-specific gene targeting provides a superior strategy that exploits homologous recombination to insert a transgene of interest into a pre-determined locus. In this study, based on bioinformatics, gene expression atlas, and Hi-C analyses, the GSH region was predicted in the chicken genome between DRG1 and EIF4ENIF1  genes. To do so, we introduce a fast and easy-to-use pipeline that allows the prediction of orthologue GSH loci in all organisms, especially chickens. In addition, the procedure to design targeting vectors for targeting these predicted GSH regions is described in detail. 

کلیدواژه‌ها

Genome safe harbor loci؛ Genetically engineered birds؛ transgenic chicken؛ CRISPR/Cas9؛ Gene expression atlas؛ Hi-C map

سایر فایل های مرتبط با مقاله

Supplementary-corrected proof-367-JCMR-1015.pdf

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