The Effect of Androgen Deprivation on the Expression of Connexin-43 mRNA in the Heart

Journal of Cell and Molecular Research

مقاله 8، دوره 13، شماره 1 - شماره پیاپی 25، آذر 2021، صفحه 65-71   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2021.71395.1010

نویسندگان

Mahnaz Ghowsi^{* 1}؛ Nazli Khajehnasiri²؛ Sajjad Sisakhtnezhad¹

¹Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran

²Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran.

چکیده

Connexin-43 (Cx-43) plays axial roles in the propagation of action potentials and contractile coupling in heart. Down-regulation of Cx-43 in heart is associated with arrhythmia, dilated cardiomyopathy and heart failure. To date, no studies have examined the effects of androgen deprivation therapy (ADT)-induced hypogonadism on the expression of Cx-43 in heart. This study investigated the effects of testosterone deprivation and its replacement with testosterone on the expression of Cx-43 mRNA and muscle-specific miRNAs miR-206 and miR-1, as two potential regulators of the Cx-43 protein expression in the ventricular tissue. Accordingly, 21 male Wistar rats were divided into three groups: Ι) Normal control, П) ORX-S: castrated rats serving as animal models for ADT and receiving the sesame oil as a solvent of testosterone enanthate for 10 weeks, and Ш) ORX-T: these animals were castrated, receiving testosterone enanthate (25 mg/kg) for 10 weeks. The relative expression of Cx-43 mRNA, miR-206 and miR-1 was determined by qRT-PCR. Cx-43 mRNA was found to be decreased in the ORX-S group. The Cx-43 mRNA was up-regulated after the administration of testosterone enanthate. There were no significant changes in miR-206 and miR-1 levels in the ORX-S and ORX-T groups when compared to the controls. Our results indicated that testosterone should be regarded as an important factor in the regulation of the Cx-43 mRNA expression in heart, and testosterone deprivation may down-regulate the Cx-43 mRNA expression; however, it doesn’t alter miR-1 and miR-206 levels. These results suggest that ADT-induced hypogonadism may put males at risk for cardiac dysfunctions.

کلیدواژه‌ها

Connexin-43؛ miR-206؛ miR-1؛ Heart؛ Testosterone؛ Androgen deprivation therapy

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