بررسی خصوصیات مورفو- فیزیولوژیکی نعناع فلفلی (Mentha piperita L.) تحت تأثیر منابع کربنی اتانول و متانول

علوم باغبانی

مقاله 3، دوره 36، شماره 1 - شماره پیاپی 53، خرداد 1401، صفحه 29-41   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jhs.2022.61778.0

نویسندگان

وحید اکبرپور^{* 1}؛ مصطفی مطهری نژاد²؛ محمدعلی بهمنیار³

¹گروه علوم و مهندسی باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

²گیاهان دارویی، مؤسسه آموزش عالی سنا، ساری، ایران

³گروه مهندسی علوم خاک، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

تغذیه گیاهان با الکل‌هایی نظیر اتانول و متانول به عنوان منابع کربنی یکی از روش‌های مناسب جهت افزایش خصوصیات کمّی و کیفی آن­ها می‌باشد. با توجه به اهمیت گیاه دارویی نعناع فلفلی (Mentha piperita L.) در تولید متابولیت‌های ثانویه باارزش و همچنین تأثیر اتانول و متانول بر برخی خصوصیات مورفولوژیکی و فیزیولوژیکی این گیاه، آزمایشی گلدانی در قالب فاکتوریل بر پایه طرح کاملاً تصادفی با سه تکرار در گلخانه تحقیقاتی دانشگاه شاهد در سال 1397 انجام شد. تیمارهای محلول‌پاشی شامل غلظت‌های مختلف صفر، 15، 30 و 45 درصد از اتانول و متانول بود. نتایج نشان داد که اتانول 45 درصد بیشترین تأثیر را بر درصد اسانس، عملکرد اسانس و درصد پروتئین داشت. ولی استفاده از تیمار 15 درصد اتانول بالاترین مقدار را در سایر صفات (ارتفاع بوته، فنل و فلاونوئید کل) به خود اختصاص داد که با تیمار 45 درصد متانول در یک سطح آماری قرار داشت. همچنین بیشترین تعداد برگ و کلروفیل a در تیمار تلفیقی 15 درصد اتانول و 15 درصد متانول مشاهده شد، در صورتی‌که بالاترین میزان کلروفیل b و کلروفیل کل مربوط به کاربرد تلفیقی 15 درصد اتانول و 45 درصد متانول بود. بنابراین، با توجه به اثر مثبت محلول‌پاشی اتانول و متانول در خصوصیات رشدی گیاه نعناع فلفلی، استفاده از این ترکیبات الکلی توصیه می‌گردد.

کلیدواژه‌ها

اتانول؛ متانول؛ متابولیت ثانویه؛ محلول‌پاشی؛ نعناع فلفلی

مراجع

Abbasian A., Mirshekari B., Safarzade Vishekaei M.N., Rashidi V., and Aminpanah H. 2016. Effects of the foliar application of methanol on the yield and growth of rice (Oryza sativa cv. Shiroudi). Ciencia e Investigacion Agrarian 43(1): 17-24. http://dx.doi.org/10.4067/S0718-16202016000100002.

2- Arnon A.N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal 23: 112-121.

3- Asgari A.A., and Moeinfard A. 2014. Effect of foliar spraying on plants as a new method in agriculture. 1^st National Congress of Biology and Natural Sciences of Iran, Tehran, Iran. (In Persian)

4- Atmodjo M.A., Hao Z., and Mohnen D. 2013. Evolving views of pectin biosynthesis. Annual Review of Plant Biology 64: 747-779. https://doi.org/10.1146/annurev-arplant-042811-105534.

5- Bagheri A., Nezami A., Ganjali A., and Parsa, M. 1997. Peas Cultivation. University of Mashhad Publications. 444 pp. (In Persian)

6- Bartley E.G., and Scolnik P.A. 1995. Plant carotenoids: pigments for photoprotection, visual attraction and human health. The Plant Cell 7: 1027-1038. https://doi.org/10.1105/tpc.7.7.1027.

7- Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-54. https://doi.org/10.1016/0003-2697(76)90527-3.

8- Chen L., Chan S., and Cossins E.A. 1997. Distribution of folate derivatives and enzymes for synthesis of 10 formyltetrahydrofolate in cytosolic and mitochondrial fractionsof pea leaves. Plant Physiology 115: 299-309. doi: 10.1104/pp.115.1.299.

9- Dai J., Orsat V., Raghavan G.S., and Yaylayan V. 2010. Investigation of various factors for the extraction of peppermint (Mentha piperita L.) leaves. Journal of Food Engineering 96: 540-3. https://doi.org/10.1016/j.jfoodeng.2009.08.037.

10- Deleris A., Halter T., and Navarro L. 2016. DNA methylation and demethylation in plant immunity. Annual Review Phytopathology 54: 579-603. DOI: 10.1146/annurev-phyto-080615-100308.

11- Dorokhov Y.L., Sheshukova E.V., and Komarova T.V. 2018. Methanol in plant life. Frontiers in Plant Science 9, 1623. doi: 10.3389/fpls.2018.01623.

12- Dorokhov Y.L., Shindyapina A.V., Sheshukova E.V., and Komarova T.V. 2015. Metabolic methanol: molecular pathways and physiological roles. Physiological Reviews 95: 603-644. DOI: 10.1152/physrev.00034.2014.

13- Ebrahimzadeh M.A., Hosseinimehr S.J., Hamidinia A., and Jafari M. 2010. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology Online 1: 7-14.

14- Gout E., Aubert S., Bligny R., Rebeille F., Nonomura A.R., Benson A.A., and Douce R. 2000. Metabolism of methanol in plant cells. Carbon-13 nuclear magnetic resonance studies. Plant Physiology 123: 287-296. doi: 10.1104/pp.123.1.287.

15- Hanson A.D., and Roje S. 2001. One carbon metabolism in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology 52: 119-138. DOI: 10.1146/annurev.arplant.52.1.119.

16- Ivanova E.G., Doronina N.V., and Trotsenko Y.A. 2001. Aerobic methylobacteria are capable of synthesizing auxins. Microbiology 70: 392-397. DOI: 10.1023/A:1010469708107.

17- Jafari Marandi S., and Majd A. 2008. The effect of alcoholic treatments (ethanol and methanol) on the development of vegetative meristem, formation of flower components, change in the number of flowering shoots, development of embryos and possibility of delaying aging in clove flowers. Quarterly Journal of Developmental Biology 1: 9-14. (In Persian with English abstract)

18- Khosravi E., Mehrafarin A., Naghdibadi H., Haji Aghaee R., and Khosravi M.T. 2012. The phytochemical response of Melissa officinalis L. to foliar application of hydro-alcoholic solutions (methanol and ethanol). Journal of Herbal Drugs 3(1): 21-25. (In Persian with English abstract)

19- Kim S.J., and Brandizzi F. 2016. The plant secretory pathway for the trafficking of cell wall polysaccharides and glycoproteins. Glycobiology, 26: 940-949. DOI: 10.1093/glycob/cww044.

20- Komarova T.V., Pozdyshev D.V., Petrunia I.V., Sheshukova E.V., and Dorokhov Y.L. 2014. Pectin methylesterase-generated methanol may be involved in tobacco leaf growth. Biochemistry (Moscow) 79: 102-110. DOI: 10.1134/S0006297914020035.

21- Kumar A., Samarth R.M., and Yasmeen S. 2004. Anticancer and radioprotective potentials of Mentha piperita L. BioFactors 22: 87-91. DOI: 10.1002/biof.5520220117.

22- Makhdum I.M., Nawaz A., Shabab M., Ahmad F., and Illahi F. 2002. Physiological response of cotton to methanol foliar application. Journal of Research, (Science) Bahauddin Zakariya University, Multan, Pakistan 13: 37-43.

23- Mehrafarin A., Naghdi Badi H., Poorhadi M., Hadavi E., Ghavami N., and Kadkhoda Z. 2011. Phytochemical and agronomic response of peppermint (Mentha piperita L.) to urea and biofertilizers application. Journal of Medicinal Plants 10(4): 107-118. (In Persian with English abstract)

24- Mehrafarin A., Naghdi Badi H., Qaderi A., Labbafi M.R., Zand E., Noormohammadi Gh., Qavami N., and Seif Sahandi M. 2015. Changes in seed yield and mucilage of Fenugreek (Trigonella foenum-graecum L.) in response to foliar application of methanol as a bio-stimulant. Journal of Medicinal Plants 2(54): 86-100. (In Persian with English abstract)

25- Moradi P., and Ebadati Esfahani R. 2016. Effect of foliar application methanol on the quality and quantity of Artemisia dracunculus L. Electronic Journal of Biology S1: 24-29.

26- Nadali I., Paknejad F., Moradi F., and Vazan S. 2010. Effect of methanol on yield and some quality characteristics of sugar beet (Beta vulgaris L.) cv. Rasoul in drought and non-drought stress conditions. Seed and Plant Production 26: 95-108. (In Persian with English abstract)

27- Nazari Moghadam R. 2014. Effect of foliar application of methanol and ethanol on essential oil content and vegetative growth of Anethum graveolens. MSc Thesis, Islamic Azad University of Miyaneh Branch, East Azerbaijan, Iran. (In Persian with English abstract)

28- Nonomura A.M., and Benson A.A. 1992. The path of carbon in photosynthesis: Improved crop yield with methanol. Proceedings of the National Academy of Sciences 89: 9794-9798. doi: 10.1073/pnas.89.20.9794.

29- Nourafkan H., and Kalantari Z. 2017. The effect of methanol and ethanol foliar application on peppermint morpho-physiological characteristics. Agroecology Journal 12(4): 1-9. (In Persian with English abstract)

30- Nourafkan H., and Pouyanfar M. 2017. Savory Morpho-physiological traits affected by methanol and ethanol foliar application. Agroecology Journal 3(1): 9-17. (In Persian with English abstract)

31- Omidbaigi R. 2005. Production and Processing of Medicinal Plants. Volume II, Astane Ghodse Razavi Publications. 438 Pp. (In Persian)

32- Pilehvari Khomami R., Safarzadeh Vishkaei M.N., Sajedi N.A., Rasooli M., and Moradi M. 2008. The effect of methanol and zinc on quantitative and qualitative parameters of peanuts in Guilan. New Finding in Agriculture 2(4): 339-351. (In Persian with English abstract)

33- Rajala A., Karkkainen J., Peltonen J., and Peltonen-Sainio P. 1998. Folia applications of alcohols failed to enhance growth and yield of C3 crops. Industrial Crops and Products 7: 129-137. DOI : 10.1016/S0926-6690(97)00041-1.

34- Ramadan T., and Omran Y.A.M. 2005. The effect of foliar application of methanol on productivity and fruit quality of grapevine cv. Flame Seedless. Vitis 44(1): 11-16. DOI: https://doi.org/10.5073/vitis.2005.44.11-16.

35- Ramirez I., Dorta F., Espinoza V., Jimenez E., Mercado A., and Pena-Cortes H. 2006. Effects of foliar and root applications of methanol on the growth of Arabidopsis, tobacco, and tomato plants. Journal of Plant Growth Regulation 25: 30-44. DOI: 10.1007/s00344-005-0027-9.

 36- Safarzadeh Vishkaee M.N. 2007. The effect of methanol on peanut growth and yield. PhD Thesis, Islamic Azad University of Science and Research of Tehran, 236 pp. (In Persian with English abstract)

37- Saffer A.M. 2018. Expanding roles for pectins in plant development. Journal of Integrative Plant Biology 60: 910-923. DOI: 10.1111/jipb.12662.

38- Sajedi Moghadam S., Mehrafarin A., Naghdi Badi H., Pazoki A.R., and Qavami N. 2012. Evaluation of phytochemical yield of Thyme (Thymus vulgaris L.) under foliar application of hydroalcohols. Journal of Medicinal Plants 11(4): 130-140. (In Persian with English abstract)

39- Shah P.P., and Mello P.M.D. 2004. A review of medicinal uses and pharmacological effects of Mentha piperita. Natural Product Radiance 3: 4.214-221.

40- Tarhan S., Telci I., Tuncay M.T. and Polatci H. 2010. Product quality and energy consumption when drying peppermint by rotary drum dryer. Industrial Crops and Products 32: 420-427. https://doi.org/10.1016/j.indcrop.2010.06.003.

41- Theodoridou A., Dornemann D., and Kotzabasis K. 2002. Light dependent induction of strongly increased microalgal growth by methanol. Biochimica et Biophysica Acta (BBA)-General Subjects 2: 189-198. DOI: 10.1016/s0304-4165(02)00438-5.

42- Yazdifar S.H., Moradi P., and Yousefi Rad M. 2015. Effect of foliar application of methanol and chelated zinc on the quantities and qualities yield of marigold (Calendula officinalis L). Journal of Applied Environmental and Biological Sciences 4: 170-176.