توسعه و ارزیابی خصوصیات لیپوزوم بهینه‌یابی شده جهت درون‌پوشانی کورکومین

مجله پژوهشهای علوم و صنایع غذایی ایران

مقاله 5، دوره 19، شماره 1 - شماره پیاپی 79، فروردین و اردیبهشت 1402، صفحه 57-78   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ifstrj.2021.73945.1118

نویسندگان

سیده فاطمه موسوی بایگی¹؛ آرش کوچکی^{* 1}؛ بهروز قرآنی²؛ محبت محبی¹

¹گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

²گروه نانوفناوری مواد غذایی، موسسه پژوهشی علوم و صنایع غذایی (RIFST)، کیلومتر 12 بزرگراه آسیایی مشهد-قوچان، صندوق پستی: 356-157-91895، مشهد، ایران

چکیده

کورکومین به‌عنوان یک ترکیب زیست فعال چربی‌دوست دارای خواص سلامتی‌بخش ویژه‌ای می‌باشد اما پایداری کم و حساسیت بالای آن موجب محدودیت دسترسی زیستی این ماده شده است. هدف از این پژوهش طراحی و توسعه سیستم حامل لیپوزومی جهت ریزپوشانی کورکومین است. درون پوشانی ترکیبات حساس از جمله کورکومین در ساختار لیپوزومی، می تواند روشی موثر بر حفاظت آنها طی شرایط هضم دستگاه گوارشی باشد. هدف از این پژوهش، ارزیابی تاثیر میزان لستین (08/0- 02/0 گرم)، نسبت لستین: کلسترول (4- 5/0)، میزان کورکومین (6- 5/1 میلی‌گرم) و زمان تیمار فراصوت (5- 1 دقیقه) بر تولید لیپوزوم‌های حاوی کورکومین بود. نمونه بهینه با آزمون‌های تعیین اندازه ذرات، توزیع اندازه ذرات، پتانسیل زتا و بازده درون پوشانی به روش تکنیک سطح پاسخ تعیین گردید و سپس ماهیت فیزیکی، ساختار مولکولی و همچنین رفتار رهایش کورکومین درون پوشانی شده در سه شرایط شبیه سازی دهان، معده و روده برای نمونه بهینه مورد ارزیابی قرار گرفت. نتایج نشان داد که همه متغیرهای مستقل بر اندازه ذرات لیپوزوم دارای اثر معنی‌دار (01/0>p) بود و افزایش نسبت لستین: کلسترول موجب یکنواختی هرچه بیشتر اندازه ذرات گردید. لستین تنها جزء اثرگذار بر پتانسیل ذرات تعیین شد و افزایش زمان امواج فراصوت موجب افزایش بازده درون پوشانی کورکومین در لیپوزوم گردید. نقطه بهینه تهیه لیپوزوم با بالاترین بازده درون پوشانی (0/1± 02/69 درصد) وکمترین اندازه ذرات (09/0± 174/214 نانومتر)، توزیع اندازه ذرات (02/0± 105/0) و پتانسیل زتا (31/0± 541/16- میلی‌ولت) در شرایط میزان لستین 08/0 گرم، نسبت لستین: کلسترول 4:1، میزان کورکومین 16/4 میلی‎‌گرم و تیمار فراصوت 5 دقیقه توسط نرم‌افزار دیزاین اکسپرت معرفی شد. کورکومین در ذرات کروی لیپوزوم بهینه به حالت آمورف بود. پایداری این لیپوزوم‌ها طی زمان تحت تاثیر دما بوده به‌طوری که در مقایسه با دمای 4درجه سانتی‌گراد، کورکومین بیشتری از لیپوزوم در دمای 25 درجه سانتی‌گراد رهایش یافته بود. همچنین رفتار رهایش کورکومین در شرایط دستگاه گوارش اساس مکانسیم انتشار بود.

کلیدواژه‌ها

بهینه‌یابی؛ رفتار رهایش؛ کورکومین؛ لیپوزوم

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