بررسی گروه‏های عاملی ترکیبات زیست‏فعال، توانایی رادیکال گیرندگی و فعالیت ضدمیکروبی عصاره آبی شنبلیله (Trigonella foenum) در شرایط برون‌تنی

مجله پژوهشهای علوم و صنایع غذایی ایران

دوره 18، شماره 4 - شماره پیاپی 76، مهر و آبان 1401، صفحه 415-426   

نوع مقاله: مقاله پژوهشی فارسی

شناسه دیجیتال (DOI): 10.22067/ifstrj.2021.72348.1092

نویسندگان

هدیه یوسفی پور¹؛ محمدامین مهرنیا^{* 2}؛ بهروز علیزاده بهبهانی¹؛ حسین جوینده²؛ محمد حجتی¹

¹گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.

²گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران

چکیده

امروزه به دلیل اثرات سمی آنتی‌اکسیدان‌ها و ترکیبات ضدمیکروبی سنتزی تمایل به استفاده از عصاره‌ها و اسانس‌ها افزایش یافته است. از این‌رو در این پژوهش میزان فنول و فلاونوئید کل عصاره آبی گیاه شنبلیله، خواص آنتی‌اکسیدانی، ضدمیکروبی و برهمکنش عصاره با آنتی‌بیوتیک‌های کلرامفنیکل و آمفوتریسین B بررسی شد. همچنین گروه‌های عاملی عصاره آبی توسط طیف‌سنجی فروسرخ تبدیل فوریه مورد مطالعه قرار گرفت. میزان فنول کل عصاره برابر با 60/64 میلی‌گرم گالیک اسید در گرم عصاره و میزان فلاونوئید کل برابر با 57/37 میلی‌گرم کوئرستین در گرم عصاره بود. میزان فعالیت آنتی‌اکسیدانی عصاره در 3 روش مهار رادیکال آزاد 1 و 1- دیفنیل- 2-پیکریل- هیدرازیل، روش مهار رادیکال آزاد 2 و 2-آزینوبیس (3- اتیل بنزوتیازولین 6- سلفونیک اسید) و روش رنگ‌بری بتاکاروتن- لینولئیک اسید به‌ترتیب برابر با 55/60، 53/55 و 40/50 درصد بود. همچنین مشخص گردید که عصاره آبی گیاه شنبلیله دارای خاصیت ضدمیکروبی خوبی بوده و برهمکنش عصاره با آنتی‌بیوتیک‌های کلرامفنیکل و آمفوتریسین B اثر سینرژیستی را در برابر میکروارگانیسم­های Escherichia coli، Enterobacter aerogenes، Staphylococcus aureus، Bacillus cereus و Candida albicans نشان داد. ازاین‌رو، عصاره آبی گیاه شنبلیله می‌تواند یک جایگزین مناسبی برای ترکیبات ضدمیکروبی و آنتی‌اکسیدانی سنتزی به شمار رود.

کلیدواژه‌ها

عصاره آبی شنبلیله؛ فعالیت ضدمیکروبی؛ خاصیت آنتی‌اکسیدانی؛ فنول و فلاونوئید کل

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