ارزیابی ویژگی‌های پروبیوتیکی و ضدقارچی مخمر جدا شده از خمیرترش باکویت

مجله پژوهشهای علوم و صنایع غذایی ایران

دوره 18، شماره 5 - شماره پیاپی 77، آذر و دی 1401، صفحه 575-588   

نوع مقاله: مقاله پژوهشی فارسی

شناسه دیجیتال (DOI): 10.22067/ifstrj.2022.74006.1119

نویسندگان

سارا شهریاری¹؛ علیرضا صادقی^{* 1}؛ مریم ابراهیمی²؛ علیرضا صادقی ماهونک¹؛ علی مویدی³

¹گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

²مرکز تحقیقات سلامت فراورده‌های غذایی، دارویی و طبیعی، دانشگاه علوم پزشکی گلستان، گرگان، ایران.

³دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

ارزیابی خصوصیات ضدمیکروبی و پروبیوتیکی فلورمیکروبی جدا شده از شبه غلات تخمیرشده جهت تامین کشت‌های میکروبی همراه و آغازگر از اهمیت به‌سزایی برخوردار است. در این پژوهش، مخمر غالب از خمیرترش باکویت، جداسازی و با استفاده از PCR شناسایی گردید. سپس ویژگی‌های پروبیوتیکی و همچنین اثر ضدقارچی این جدایه مخمری بر روی Aspergillus niger وAspergillus flavus مورد مطالعه قرارگرفت. توالی‌یابی محصولات PCR منجر به شناسایی مخمر Pichia kudriavzevii به‌عنوان جدایه مخمری خمیرترش باکویت شد. نرخ زنده‌مانی جدایه مذکور پس از تیمار متوالی اسید و صفرا در مقایسه با نمونه شاهد 26/79 درصد بود. همچنین اثر ضدباکتریایی آن در برابر Escherichia coli نسبت به سایر عوامل غذازاد مورد مطالعه به شکل معنی‌داری (05/0p<) بیشتر بود. با این حال، رشد Listeria monocytogenes در حضور جدایه مخمری 50/19 درصد کاهش یافت اما این جدایه، اثر بازدارنده‌ای بر Salmonella entrica نداشت. قابلیت‌های آبگریزی و خوداتصالی جدایه مذکور نیز به‌ترتیب، 07/64 و 40/67 درصد تعیین گردید. علاوه بر این، جدایه مخمری نسبت به تمامی آنتی‌بیوتیک‌های مورد بررسی، مقاومت نشان داد اما در برابر آنتی‌مایکوتیک‌های کتوکونازول و فلوکونازول، مقاوم و همچنین نسبت به ایتراکونازول دارای حساسیت نسبی بود. این جدایه مخمری، فاقد فعالیت همولیتیکی بود و اثر ضدقارچی آن در برابر A. niger و A. flavus نیز مورد تایید قرار گرفت. بر این اساس، جدایه P. kudriavzevii از قابلیت مناسبی برای استفاده به‌عنوان کشت پروبیوتیک و یا محافظت کننده در صنایع تخمیری برخوردار می‌باشد.

کلیدواژه‌ها

جدایه مخمری؛ خمیرترش باکویت؛ ویژگی‌های پروبیوتیکی؛ اثر ضدقارچی

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