استخراج آنزیمی پلی‌ساکارید سولفاته از پوست ماهی قزل‌آلای رنگین کمان (Oncorhynchus mykiss) و بررسی شیمیایی، خواص ضداکسیدانی و کارکردی آن

مجله پژوهشهای علوم و صنایع غذایی ایران

دوره 18، شماره 5 - شماره پیاپی 77، آذر و دی 1401، صفحه 725-738   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/ifstrj.2022.72999.1102

نویسندگان

شهاب نقدی¹؛ مسعود رضائی^{* 1}؛ مهدی عبدالهی²؛ مهدی طبرسا¹

¹گروه فرآوری محصولات شیلاتی، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس، نور، ایران.

²گروه زیست‌شناسی و مهندسی بیولوژیکی، علوم غذایی و تغذیه، دانشگاه صنعتی چالمرز، گوتنبرگ، سوئد.

چکیده

در مطالعه حاضر پلی‌ساکارید سولفاته از پوست ماهی قزل‌آلا توسط آنزیم پپسین استخراج شد و آنالیزهای FTIR، کربوهیدرات، سولفات، یورونیک اسید و پروتئین آن انجام شد. نتایج آنالیز شیمیایی پلی‌ساکاریدهای استخراج شده نشان داد که بازده استخراج 02/0 ±23/3% بود، همچنین درصد کربوهیدارت و پروتئین نمونه به‌دست آمده 56/2 ±03/57، 43/0 ±78/7 بود. همچنین مقدار سولفات و یورونیک اسید به‌ترتیب 77/0 ±54/6 و 43/0 ±86/3 بود. نتایج طیف‌سنجی مادون قرمز نشان داد یک پیک پهن در ناحیه 3350 تا cm^-1 3450 مربوط به گروه –OH و باند خمشی سولفات S=O در ناحیه cm^-1 1245 ظهور پیدا کرد. یک روند افزایشی و معنی‌دار در غلظت‌های مختلف مورد استفاده برای تست DPPH مشاهده شد (p<0.05) که بالاترین قدرت خنثی‌کنندگی (85/38%) در غلظت 2 میلی‌گرم/ میلی‌لیتر مشاهده شد. بالاترین درصد شلاته‌کنندگی رادیکال ABTS در غلظت 4 میلی‌گرم بر میلی‌لیتر آب مقطر با درصد (70/71%) مشاهده شد (p<0.05). نتایج شلاته‌کنندگی یون‌های فروزین پلی‌ساکارید استخراج شده نشان داد که بالاترین درصد شلاته‌کنندگی 43/98% بود (p<0.05). ظرفیت کف‌کنندگی، خواص پایداری کف و ظرفیت امولیسیون‌کنندگی نمونه مورد مطالعه حاکی از یک روند افزایشی با افزایش غلظت نمونه بود (p<0.05) و غلظت 10% پلی‌ساکارید سولفاته استفاده شده بالاترین درصد کف‌کنندگی (22/72%) و پایداری کف (22/62%) را از خود نشان داده است (p<0.05). خاصیت امولیسیون‌کنندگی پلی‌ساکارید سولفاته استخراج شده در برابر روغن سویا در تمامی غلظت‌های استفاده شده بیشتر از روغن آفتابگردان بود (p<0.05) و بالاترین مقدار این خاصیت مربوط به غلظت 10% با عدد 57/86% و 59/92% به‌ترتیب در برابر روغن آفتابگردان و روغن سویا بود (p<0.05).   

کلیدواژه‌ها

ماهی قزل‌آلای رنگین کمان (Oncorhynchus mykiss)؛ پلی‌ساکارید سولفاته؛ ضداکسیدانی؛ کف‌کنندگی؛ امولیسیون‌کنندگی

مراجع

1. Alboofetileh, M., Rezaei, M., & Tabarsa, M. (2018). Enzyme-assisted extraction of Nizamuddinia zanardinii for the recovery of sulfated polysaccharides with anticancer and immune-enhancing activities. Journal of Applied Phycology. https://doi.org/10.1007/s10811-018-1651-7
2. Alboofetileh, M., Rezaei, M., Tabarsa, M., & You, S. (2019). Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods. Journal of Food Science and Technology. https://doi.org/10.1007/s13197-019-03584-1
3. Arima, K., Fujita, H., Toita, R., Imazu-Okada, A., Tsutsumishita-Nakai, N., Takeda, N., Nakao, Y., Wang, H., Kawano, M., Matsushita, K., Tanaka, H., Morimoto, S., Nakamura, A., Kitagaki, M., Hieda, Y., Hatto, R., Watanabe, A., Yumura, T., Okuhara, T., … Tamura, J. I. (2013). Amounts and compositional analysis of glycosaminoglycans in the tissue of fish. Carbohydrate Research, 366, 25–32. https://doi.org/10.1016/j.carres.2012.11.010
4. Bitter, T., & Muir, H. M. (1962). A modified uronic acid carbazole reaction. In Analytical Biochemistry (Vol. 4, Issue 4, pp. 330–334). https://doi.org/10.1016/0003-2697(62)90095-7
5. Borazjani, N. J., Tabarsa, M., You, S. G., & Rezaei, M. (2017). Effects of extraction methods on molecular characteristics, antioxidant properties and immunomodulation of alginates from Sargassum angustifolium. International Journal of Biological Macromolecules, 101, 703–711. https://doi.org/10.1016/j.ijbiomac.2017.03.128
6. Chang, S. C., Hsu, B. Y., & Chen, B. H. (2010). Structural characterization of polysaccharides from Zizyphus jujuba and evaluation of antioxidant activity. International Journal of Biological Macromolecules, 47(4), 445–453. https://doi.org/10.1016/j.ijbiomac.2010.06.010
7. Chen, G., Fang, C., Chen, X., Wang, Z., Liu, M., & Kan, J. (2019). High-pressure ultrasonic-assisted extraction of polysaccharides from Mentha haplocalyx: Structure, functional and biological activities. Industrial Crops and Products, 130(October 2018), 273–284. https://doi.org/10.1016/j.indcrop.2018.12.086
8. Chew, K. K., Ng, S. Y., Thoo, Y. Y., Khoo, M. Z., Wan Aida, W. M., & Ho, C. W. (2011). Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Centella asiatica International Food Research Journal, 18(2), 571–578.
9. Cho, C. W., Han, C. ji, Rhee, Y. K., Lee, Y. C., Shin, K. S., Shin, J. S., Lee, K. T., & Hong, H. Do. (2015). Cheonggukjang polysaccharides enhance immune activities and prevent cyclophosphamide-induced immunosuppression. International Journal of Biological Macromolecules, 72, 519–525. https://doi.org/10.1016/j.ijbiomac.2014.09.010
10. Dong, X., Duan, X., Sun, Z., Zhang, X., Li, C., Yang, S., Ren, B., Zheng, S., & Dionysiou, D. D. (2019). Structural characterization and antioxidant activities of a water soluble polysaccharide isolated from Glycyrrhiza glabra. “Applied Catalysis B, Environmental,” 118214. https://doi.org/10.1016/j.apcatb.2019.118214
11. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350–356. https://doi.org/10.1021/ac60111a017
12. FAO (2018), Yearbook of Fishery Statistics, Statistics and Information Service, FAO Fisheries and Aquaculture Department. Viale delle Terme di Caracalla 00153, Rome: Food and Aquaculture Organization of the United Nations. http://www.fao.org/fishery/statistics/en.
13. Ghribi, A. M., Sila, A., Gafsi, I. M., Blecker, C., Danthine, S., Attia, H., ... & Besbes, S. (2015). Structural, functional, and ACE inhibitory properties of water-soluble polysaccharides from chickpea flours. International Journal of Biological Macromolecules, 75, 276-282. https://doi.org/10.1016/j.ijbiomac.2015.01.037
14. Gilar, M., Yu, Y. Q., Ahn, J., Xie, H., Han, H., Ying, W., & Qian, X. (2011). Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectrometry. Analytical Biochemistry, 417(1), 80–88. https://doi.org/10.1016/j.ab.2011.05.028
15. Gomaa, M., Hifney, A. F., Fawzy, M. A., & Abdel-Gawad, K. M. (2018). Use of seaweed and filamentous fungus derived polysaccharides in the development of alginate-chitosan edible films containing fucoidan: Study of moisture sorption, polyphenol release and antioxidant properties. In Food Hydrocolloids (Vol. 82). Elsevier Ltd. https://doi.org/10.1016/j.foodhyd.2018.03.056 
16. Grina, F., Ullah, Z., Kaplaner, E., Moujahid, A., Eddoha, R., Nasser, B., Terzioğlu, P., Yilmaz, M. A., Ertaş, A., Öztürk, M., & Essamadi, A. (2020). In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical fingerprints of five Moroccan seaweeds. South African Journal of Botany, 128, 152–160. https://doi.org/10.1016/j.sajb.2019.10.021 
17. Guo, L., Zhu, W., Xu, F., Liu, M., Xie, Y., & Zhang, J. (2014). Optimized ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis and evaluation of antioxidant activities in vitro. CYTA- Journal of Food, 12(1), 32–39. https://doi.org/10.1080/19476337.2013.785982 
18. Hamzaoui, A., Ghariani, M., Sellem, I., Hamdi, M., Feki, A., Jaballi, I., Nasri, M., & Amara, I. Ben. (2020). Extraction, characterization and biological properties of polysaccharide derived from green seaweed “Chaetomorpha linum” and its potential application in Tunisian beef sausages. International Journal of Biological Macromolecules, 148, 1156–1168. https://doi.org/10.1016/j.ijbiomac.2020.01.009
19. Joana Gil-Chávez, G., Villa, J. A., Fernando Ayala-Zavala, J., Basilio Heredia, J., Sepulveda, D., Yahia, E. M., & González-Aguilar, G. A. (2013). Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: An Overview. Comprehensive Reviews in Food Science and Food Safety, 12(1), 5–23. https://doi.org/10.1111/1541-4337.12005
20. Jridi, M., Mezhoudi, M., Abdelhedi, O., Boughriba, S., Elfalleh, W., Souissi, N., Nasri, R., & Nasri, M. (2018). Bioactive potential and structural characterization of sulfated polysaccharides from Bullet tuna (Auxis Rochei) by-products. Carbohydrate Polymers, 194(April), 319–327. https://doi.org/10.1016/j.carbpol.2018.04.038
21. Jridi, M., Nasri, R., Marzougui, Z., Abdelhedi, O., Hamdi, M., & Nasri, M. (2019). Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle. International Journal of Biological Macromolecules, 123, 1221–1228. https://doi.org/10.1016/j.ijbiomac.2018.11.170
22. Khan, B. M., Qiu, H. M., Wang, X. F., Liu, Z. Y., Zhang, J. Y., Guo, Y. J., Chen, W. Z., Liu, Y., & Cheong, K. L. (2019). Physicochemical characterization of Gracilaria chouae sulfated polysaccharides and their antioxidant potential. International Journal of Biological Macromolecules, 134, 255–261. https://doi.org/10.1016/j.ijbiomac.2019.05.055
23. Leroux, J., Langendorff, V., Schick, G., Vaishnav, V., & Mazoyer, J. (2003). Emulsion stabilizing properties of pectin. Food Hydrocolloids, 17(4), 455–462. https://doi.org/10.1016/S0268-005X(03)00027-4
24. Li, H., Tao, Y., Zhao, P., Zhi, D., Gao, X., Zhao, X., & Li, M. (2019). Effect of ultrasound-assisted extraction on physicochemical properties and TLR2-affinity binding of the polysaccharides from Pholiota nameko. International Journal of Biological Macromolecules, 135, 1020–1027. https://doi.org/10.1016/j.ijbiomac.2019.05.177
25. Li, X., & Wang, L. (2016). Effect of extraction method on structure and antioxidant activity of Hohenbuehelia serotina polysaccharides. International Journal of Biological Macromolecules, 83, 270–276. https://doi.org/10.1016/j.ijbiomac.2015.11.060
26. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 193(1), 265–275. https://doi.org/10.1016/0304-3894(92)87011-4
27. Loyd, A. G., Dogson, K. S., Price, R. G., & Rose, F. A. (1960). Polysaccharide Sulphates. Biochem Biophys Acta, 46(2961).
28. Odeleye, T., White, W. L., & Lu, J. (2019). Extraction techniques and potential health benefits of bioactive compounds from marine molluscs: a review. Food & Function. https://doi.org/10.1039/c9fo00172g
29. Bhuyar, M.H. Rahim, S. Sundararaju, G.P. Maniam, N. G. (2020). Antioxidant and antibacterial activity of red seaweed; Kappaphycus alvarezii against pathogenic bacteria. Global Journal of Environmental Science and Management, 5(1), 47–58. https://doi.org/10.22034/gjesm.2020.01.0
30. Pan, X. Y., Wang, Y. M., Li, L., Chi, C. F., & Wang, B. (2019). Four antioxidant peptides from protein hydrolysate of red stingray (dasyatis akajei) cartilages: Isolation, identification, and in vitro activity evaluation. Marine Drugs, 17(5). https://doi.org/10.3390/md17050263
31. Pomin, V. H., & Mulloy, B. (2018). Glycosaminoglycans and proteoglycans. Pharmaceuticals, 11(1), 1–9. https://doi.org/10.3390/ph11010027
32. Qi, H., Zhao, T., Zhang, Q., Li, Z., Zhao, Z., & Xing, R. (2005). Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta). Journal of Applied Phycology, 17(6), 527–534. https://doi.org/10.1007/s10811-005-9003-9
33. Rjeibi, I., Hentati, F., Feriani, A., Hfaiedh, N., Delattre, C., Michaud, P., & Pierre, G. (2019). Novel antioxidant, anti-α-amylase, anti-inflammatory and antinociceptive water-soluble polysaccharides from the aerial part of Nitraria retusa. Foods, 9(1), 28. https://doi.org/10.3390/foods9010028
34. Romdhane, M. Ben, Haddar, A., Ghazala, I., Jeddou, K. Ben, Helbert, C. B., & Ellouz-Chaabouni, S. (2017). Optimization of polysaccharides extraction from watermelon rinds: Structure, functional and biological activities. Food Chemistry, 216, 355–364. https://doi.org/10.1016/j.foodchem.2016.08.056
35. Saravana, P. S., Cho, Y. J., Park, Y. B., Woo, H. C., & Chun, B. S. (2016). Structural, antioxidant, and emulsifying activities of fucoidan from Saccharina japonica using pressurized liquid extraction. Carbohydrate Polymers, 153, 518–525. https://doi.org/10.1016/j.carbpol.2016.08.014
36. Sciarini, L. S., Maldonado, F., Ribotta, P. D., Pérez, G. T., & León, A. E. (2009). Chemical composition and functional properties of Gleditsia triacanthos Food Hydrocolloids, 23(2), 306–313. https://doi.org/10.1016/j.foodhyd.2008.02.011
37. Shahidi, F. (2012). Nutraceuticals, functional foods and dietary supplements in health and disease. Journal of Food and Drug Analysis, 20(SUPPL.1), 226–230.
38. Shen, Q., Zhang, C., Jia, W., Qin, X., Cui, Z., & Mo, H. (2019). Co-production of chondroitin sulfate and peptide from lique fied chicken sternal cartilage by hot-pressure. Carbohydrate Polymers, 222(April), 115015. https://doi.org/10.1016/j.carbpol.2019.115015
39. Soua, L., Koubaa, M., Barba, F. J., Fakhfakh, J., Ghamgui, H. K., & Chaabouni, S. E. (2020). Water-Soluble Polysaccharides from Ephedra alata Stems: Structural characterization, functional properties, and antioxidant activity. Molecules, 25(9), 1–18. https://doi.org/10.3390/molecules25092210
40. Souissi, N., Boughriba, S., Abdelhedi, O., Hamdi, M., Jridi, M., Li, S., & Nasri, M. (2019a). Extraction, structural characterization, and thermal and biomedical properties of sulfated polysaccharides from razor clam Solen marginatus. RSC Advances, 9(20), 11538–11551. https://doi.org/10.1039/C9RA00959K
41. Trigui, I., Yaich, H., Sila, A., Cheikh-Rouhou, S., Bougatef, A., Blecker, C., Attia, H., & Ayadi, M. A. (2018). Physicochemical properties of water-soluble polysaccharides from black cumin seeds. International Journal of Biological Macromolecules, 117, 937–946. https://doi.org/10.1016/j.ijbiomac.2018.05.202
42. Wang, Y., Ghosh, S., & Nickerson, M. T. (2019). Effect of pH on the formation of electrostatic complexes between lentil protein isolate and a range of anionic polysaccharides, and their resulting emulsifying properties. Food Chemistry, 298(June), 125023. https://doi.org/10.1016/j.foodchem.2019.125023
43. Xie, J. H., Tang, W., Jin, M. L., Li, J. E., & Xie, M. Y. (2016). Recent advances in bioactive polysaccharides from Lycium barbarum , Zizyphus jujuba Mill, Plantago spp., and Morus spp.: Structures and functionalities. Food Hydrocolloids, 60(235), 148–160. https://doi.org/10.1016/j.foodhyd.2016.03.030
44. Xu, S. Y., Huang, X., & Cheong, K. L. (2017a). Recent advances in marine algae polysaccharides: Isolation, structure, and activities. Marine Drugs, 15(12), 1–16. https://doi.org/10.3390/md15120388
45. Yang, L., & Zhang, L. (2009). Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources. Carbohydrate Polymers, 76(3), 349–361. https://doi.org/10.1016/j.carbpol.2008.12.015
46. Ye, Q., Georges, N., & Selomulya, C. (2018). Microencapsulation of active ingredients in functional foods: From research stage to commercial food products. Trends in Food Science and Technology, 78(January), 167–179. https://doi.org/10.1016/j.tifs.2018.05.025
47. Yu, Y., Shen, M., Song, Q., & Xie, J. (2018). Biological activities and pharmaceutical applications of polysaccharide from natural resources: A review. Carbohydrate Polymers, 183(235), 91–101. https://doi.org/10.1016/j.carbpol.2017.12.009
48. Zamora-Sillero, J., Gharsallaoui, A., & Prentice, C. (2018). Peptides from Fish By-product Protein Hydrolysates and Its Functional Properties: an Overview. Marine Biotechnology, 20(2), 118–130. https://doi.org/10.1007/s10126-018-9799-3
49. Zhu, D. Y., Ma, Y. L., Wang, C. H., Wang, H., Ren, Y. F., Zhang, J. G., Thakur, K., & Wei, Z. J. (2017). Insights into physicochemical and functional properties of polysaccharides sequentially extracted from onion (Allium cepa). International Journal of Biological Macromolecules, 105, 1192–1201. https://doi.org/10.1016/j.ijbiomac.2017.07.164