بررسی ویژگی‌های کیفی و ایمنی کلاژن پوست گونه‌های سرگنده (Hypophthalmichthys nobilis)، معمولی(Cyprinus carpio) ، علف‌خوار (Ctenopharyngodon idella) و نقره‌ای (Hypophthalmichthys molitrix) کپور ماهیان پرورشی

مجله پژوهشهای علوم و صنایع غذایی ایران

مقاله 3، دوره 19، شماره 3 - شماره پیاپی 81، مرداد و شهریور 1402، صفحه 33-47   

نوع مقاله: مقاله پژوهشی لاتین

شناسه دیجیتال (DOI): 10.22067/ifstrj.2023.81732.1245

نویسنده

مینا سیف زاده^*

مرکز ملی تحقیقات فرآوری آبزیان، پژوهشکده آبزی‌پروری آب‌های داخلی، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، انزلی، ایران

چکیده

تنوع زیاد کاربردهای کلاژن و نقش مهم آن در آینده جامعه، سبب شد که به پلیمر زیستی کلیدی برای سلامت و رفاه انسان تبدیل شود. بنابراین مطالعه حاضر با هدف استخراج کلاژن از پوست ماهی کپور پرورشی، تعیین خصوصیات کیفی کلاژن و مقایسه آنها انجام شد. کلاژن از پوست ماهی کپور به روش آنزیمی اسیدی با استفاده از اسید استیک 5/0مولار و پپسین طی مدت زمان 48 ساعت استخراج شد. تیمارهای کلاژن (5 تیمار) شامل کلاژن تهیه شده از پوست گونه‌های معمولی، علف‌خوار، سر گنده و نقره‌ای کپور ماهیان و گاو (شاهد) هستند. تیمارهای کلاژن قادر به همولیز خون نبودند و اثرات سمی روی سلول‌های فیبروبلاست انسانی نشان ندادند. فلزات سنگین (18/0–01/0 ppm) در کلاژن استخراج شده از گونه‌های کپور پرورشی در محدوده استاندارد قرار داشتند. رنگ (روشنایی) کلاژن آزمایشی (68/93–74/92) و شاهد (38/92) تفاوت معنی‌داری نشان ندادند (05/0 <P). اسیدهای آمینه سیستئین و تریپتوفان در کلاژن مشاهده نشد. اسیدهای آمینه گلیسین و هیدروکسی لیزین (به‌ترتیب 352 و 3 residues 1000g^-1) بیشترین و کمترین میزان کلاژن را داشتند. پروفایل اسیدهای آمینه و راندمان تولید کلاژن (59/10-51/10 درصد) تفاوت معنی‌داری را در گونه‌های ماهی کپور نشان ندادند (05/0 <P). بر اساس نتایج مطالعه حاضر، بین راندمان تولید، ایمنی و ویژگی‌های کیفی کلاژن در گونه‌های کپور پرورشی تفاوت معنی‌داری مشاهده نشد (05/0 <P). این ویژگی‌ها بین کلاژن آزمایشی و شاهد تفاوت معنی‌داری نشان ندادند (05/0 <P). از این رو پوست این گونه‌ها را می‌توان برای تولید کلاژن به کار گرفت و جایگزین کلاژن پستانداران کرد.

کلیدواژه‌ها

آنزیم پپسین؛ ایمنی؛ کپور ماهیان پرورشی؛ کلاژن؛ هیدرولیز اسیدی

مراجع

1. Ahmed, R., Haq, M., & Chun, B.S. (2019). Characterization of marine derived collagen extracted from the by-products of bigeye tuna (Thunnus obesus). International Journal Biological Macromoleculs, 135(1), 668–676. https://doi.org/10.1016/j.ijbiomac.2019.05.213
2. Andini, A., Prayekti, E., Wulandari, D.D., & Nidianti, E. (2020). Cytotoxicity assay using brine shrimp lethality test on collagen chitosan wound dressing sterilized by ultraviolet Light. Indonesian Journal of Medical Laboratory Science and Technology, 2(1), 21-26. https://doi.org/10.33086/ijmlst.v2i1.1467
3. Association of Official Analytical Chemists (AOAC). (2000). Fish and Marine Products Treatment and Preparation of Sample. AOAC Standard No. 07. Washington, DC: U.S. Patent and Trademark Office.
4. Arumugam, G.K.S., Sharma, D., Balakrishnan, R.M., Babu, J., & Ettiyappan, P. (2018). Extraction, optimization, and characterization of collagen from sole fish skin. Sustainable Chemistry and Pharmacy, 9(2018), 19–26. https://idr.nitk.ac.in/jspui/handle/123456789/11243
5. Blanco, M., Vázquez, J.A., Pérez-Martín, R.I., & Sotelo, C.G. (2017). Hydrolysates of fish skin collagen: an opportunity for valorizing fish industry byproducts. Marine Drugs, 15(5), https://doi.org/10.3390/md15050131
6. Carvalho, A.M., Marques, A.P., Silva, T.H., & Reis, R.L. (2018). Evaluation of the potential of collagen from Codfish skin as a biomaterial for biomedical applications. Marine Drugs, 16(12), https://doi.org/10.3390/md16120495
7. Cao, J., Duan, Q., Liu, X., Shen, X., & Li, C. (2019). Extraction and physicochemical characterization of pepsin soluble collagens from golden Pompano (Trachinotus blochii) skin and bone. Journal Aquatic Food Product Technology, 28(3), 837–847. https://doi.org/10.1080/10498850.2019.1652216
8. Chinh, N.T., Manh, V.Q., Trung, V.Q., Lam, T.D., Huynh, M.D., Tung, N.Q., Trinh, N.D., & Hoang, T. (2019). Characterization of collagen derived from tropical freshwater Carp fish scale Wastes and its amino acid sequence. Natural Product Communications, 14(7), 1–12. https://doi.org/1177/1934578X19866288
9. Chotphruethipong, L., Hutamekalin, P., Nilsuwan, K., Sukketsiri, W., Aluko, R.E., Abdul, N.R., & Benjakul, S. (2022). Combined effects of defatted hydrolyzed collagen from salmon skin and vitamin C on proliferation and migration of human fibroblast cell. Fishes, 7(5), https://doi.org/10.3390/ fishes7050265
10. Chuaychan, S. (2016). Production and characterization of collagen, gelatin, and gelatin hydrolysate powder from scales of spotted golden Goatfish. Feculty of Food Science and Technology, Prince of Songkla University, Songkhla, Thailand.
11. Cumming, M.H., Hall, B., & Hofman, K. (2019). Isolation and characterisation of major and minor collagens from Hyaline Cartilage of Hoki (Macruronus novaezelandiae). Marine Drugs, 17(4), https://doi.org/10.3390/md17040223
12. Devran, D., & Gulay, O.Y. (2023). Investigation of possible heavy metals and antibiotic residues in commercial collagen. Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi, 8(1), 31–36. https://doi.org/24880/maeuvfd.1218939
13. Junianto, J., Iskandar, I., Rizal, A., & Damayanti, W. (2018). The influence of concentration of acetic acid and pepsin enzyme in Nilem fish skin collagen extractionto the amount of rendement produced. World News Natural Science, 21(2018), 164–170.
14. Ferreira, A.C., Bomfim, M.R.Q., & da Costa Sobrinho, C.H.d.B.(2022). Characterization, antimicrobial and cytotoxic activity of polymer blends based on chitosan and fish collagen. AMB Express, 12(1), https://doi.org/10.1186/s13568-022-01433-7
15. García-Sifuentes, C.O., Lugo-Sánchez, M.E., Scheuren-Acevedo, S., Martínez-Porchas, M., & Peralta-Martínez, V. (2016). Amino acid profile of collagen fractions extracted from by-products of Ophistonemalibertate and Scomberjaponicus, CyTA. Journal of Food, 14(2), 296-301. https://doi.org/10.1080/19476337.2015.1101711
16. Gauza-Włodarczyk, M., Kubisz, , & Włodarczyk, D. (2017). Amino acid composition in determination of collagen origin and assessment of physical factors effects. International Journal Biology Macromolecule, 104(PtA), 987–991. https://doi.org/10.1016/j.ijbiomac.2017.07.013
17. Gilbert,W. (2014). Applying the hedonic method. National Institute of Standards and Technology Technical Note 1811. https://doi.org/10.6028/nist.tn.1811
18. Iranian National Standard No. 12014. (1999). Heavy metal measurement of cosmetic products. Iranian Standard and Industrial Research Institute.
19. Iranian National Standard No. 10699. (2015). Livestock, poultry, and aquatic feed - measuring the amount of amino acids. Iranian Standard and Industrial Research Institute.
20. Gómez‑Limia, L., Carballo, J., Rodríguez‑González, M., & Martínez, S. (2022). Proximate composition and amino acid profle of European eel skin: infuence of body weight. European Food Research and Technology, 248(1053–1063), 1437–1446. https://doi.org/10.1007/s00217-022-03978-0
21. Govindharaj, M., Roopavath, U.K., & Rath, S.N. (2019). Valorization of discarded Marine Eel fish skin for collagen extraction as a 3D printable blue biomaterial for tissue engineering. Journal of Cleaner Production, 230(2), 412–419. https://doi.org/1016/j.jclepro.2019.05.082
22. ISO 10993-6. (2016). Biological evaluation of medical devices. Part 6: tests for local effects after implantation.
23. Li, H., Cheng, W., & Liu, K. (2017). Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects. Carbohydr Polym,165(2), 30–38. https://doi.org/1016/j.carbpol.2017.02.023
24. Lin, X., Chen, Y., Jin, H., Zhao, Q., Liu, C., Li, R., Yu, F., Chen, Y., Huang, F., Yang, Z., Ding, G., & Tang, Y. (2019). Collagen extracted from bigeye Tuna skin by isoelectric precipitation: physicochemical properties, proliferation, and migration activities. Marine Drugs, 17(5), 261. https://doi.org/10.3390/md17050261
25. Menezes, M.D.L.L.R., Ribeiro, H.L., Flávia de Oliveira, M., & de Andrade Feitosa, J.P. (2020). Optimization of the collagen extraction from Nile tilapia skin (Oreochromis niloticus) and its hydrogel with hyaluronic acid. Colloids and Surfaces B: Biointerfaces, 189(2), https://doi.org/10.1016/j.colsurfb.2020.110852
26. Momtahan, N., Panahi, T., Poornejad, N.,  Stewart, M.G., Vance, B.R., Struk, J.A., Castleton, A.A., Roeder, B.L., Sukavaneshvar, S., & Cook, A.D. (2016). Using hemolysis as a novel method for assessment of cytotoxicity and blood compatibility of decellularized heart tissues. ASAIO Journal, 62(3), 340-8. https://doi.org/10.1097/MAT.0000000000000373
27. Moses, S., Agbaji, E.B., Ajibola, V.O., & Gimba, C.E. (2018). Amino acid composition and proximate analysis in Tilapia (Oreochromis mossambicus) Fish from dams and rivers in Zamfara state, Nigeria. Journal Applied Science Environment Management, 22(6), 8899–905. https://doi.org/4314/jasem.v22i6.10
28. Mullen, A., Bayon, Y., Pandit, A., & Raghunath, M. (2019). The collagen suprafamiliy: From biosynthesis to advanced biomaterial development. Advanced Materials, 31(1), 6–11. https://doi.org/10.1002/adma.201801651
29. Nalinanon, S., Benjakul, S., Visessanguan, W., & Kishimura, H. (2007). Use of pepsin for collagen extraction from the skin of bigeye snapper (Priacanthus tayenus). Food Chemistry, 104(2), 593-601. https://doi.org/10.1016/j.foodchem.2006.12.035.
30. Oslan, S.N.H., Shapawi, R., Mokhtar, R.A.M., Noordin,W.N.M., & Huda, N. (2022). Characterization of acid and pepsin-soluble collagen extracted from the skin of purple-Spotted Bigeye Snapper. Gels, 8(10), 665. https://doi.org/10.3390/gels8100665
31. Qiu, B., Wei, F., Sun, X., Wang, X., Duan, B., Shi, C., Zhang, J., Zhang, J., Qiu, W., & Mu, W. (2014). Measurement of hydroxyproline in collagen with three different methods. Molecular Medicine Reports, 10(2), 1157–1163. https://doi.org/10.3892/mmr.2014.2267
32. Raman, M., & Gopakumar, K. (2018). Fish collagen and its applications in food and pharmaceutical industry: A review. EC Nutrition, 13(2018), 752–767. https://api.semanticscholar.org/CorpusID:54176016
33. Rosmawati, Abustam, E., Tawali, A., & Said, M.I. (2018). Amino acid and collagen content of Snakehead (Channa striata) fish skin and bone. Scientific Research Journal, 6(1), https://api.semanticscholar.org/CorpusID:212409859
34. Ramle, S.Z., Oslan, S.N.H., Shapawi, R., Mokhtar, R.A.M., Noordin, W.N.M., & Huda, N. (2022). Biochemical characteristics of acid-soluble collagen from food processing by-products of Needlefish Skin (Tylosurus acus melanotus). Appliued Science, 12(24), 12695. https://doi.org/ 10.3390/app122412695
35. Seifzadeh, M., Golashahi, E., & Safiyari, Sh. (2018). Study the concentrations of lead and cadmium in farmed rainbow trout in Talesh of Guilan. Animal Science Research Journal, 28(2), 65–79. https://sid.ir/paper/365590/en
36. Song, W.K., Liu, D., Sun, L.L., Li, B.F., & Hou, H. (2019). Physicochemical and biocompatibility properties of type I collagen from the skin of Nile Tilapia (Oreochromis niloticus) for biomedical applications. Marine Drugs, 17(3), 137. https://doi.org/10.3390/md17030137
37. Sorushanova, A., Delgado, L., Zhuning, W., Shologu, N., Kshirsagar, A., Raghunath, R., Sousa, R.O., Alves, A. L., Carvalho, D.N., Martins, E., Oliveira, C., Silva, T.H., & Reis, R.L. (2020). Acid and enzymatic extraction of collagen from Atlantic cod (Gadus morhua) swim bladders envisaging health-related applications. Journal of Biomaterials Science, Polymer Edition, 31(3), 20–37. https://doi.org/10.1080/09205063.2019.1669313
38. Sukhikh, S., Noskova, S., Ivanova, S., Ulrikh, E., Izgaryshev, A., Larichev, T., Kozlova, O., Prosekov, A., & Babich, O. (2021). Comparative analysis of collagen-containing waste biodegradation, amino acid, peptide and carbohydrate composition of hydrolysis products. Applied Science, 11(23), 11511. https://doi.org/10.3390/app112311511
39. Tan, Y., & Chang, S.K.C. (2018). Isolation and characterization of collagen extracted from channel catfish (Ictalurus punctatus) skin. Food Chemistry, 242(1), 147–155. https://doi.org/10.1016/j.foodchem.2017.09.013
40. Xu, N., Peng, X.L, Li, H.R., Liu, J.X., Cheng, J.S.Y., Qi, X,Y., Ye, S.J, Gong, H.L, Zhao, X.H., Yu, J., Xu, G., & Wei, D.X. (2021). Marine-derived collagen as biomaterials for human health. Frontires Nutrition, 8(3), 702108. https://doi.org/10.3389/fnut.2021.702108
41. Zhang, W., Zheng, J., Tian, X., Tang, Y., Ding, G., Yang, Z., & Jin, H. (2019). Pepsin-soluble collagen from the skin of Lophius litulo: A preliminary study evaluating physicochemical, antioxidant, and wound healing properties. Marine Drugs, 17(12), 708. https://doi.org/10.3390/md17120708
42. Wang, T., Yang, L., Wang, G., Han, L., Chen, K., Liu, P., Xu, S., Li, D., Xie, Z., Mo, X., Wang, L., Liang, H., Liu, X., Zhang, S., & Gao, Y. (2021). Biocompatibility, hemostatic properties, and wound healing evaluation of tilapia skin collagen sponges. Journal of Bioactive and Compatible Polymers, 36(1), 44–58. https://doi.org/10.1177/0883911520981705
43. Weber, M., Steinle, H., Golombek, S., Hann, L., Schlensak, C., Wendel, H. P., & Avci-Adali, M. (2018). Blood-Contacting biomaterials: in vitro evaluation of the Hemocompatibility. Frontires Bioenginering Biotechnology, 6(3), 99. https://doi.org/10.3389/fbioe.2018.00099
44. Wei, P., Zheng, H., Shi, Z., Li, D., & Xiang, Y. (2019). Isolation and characterization of acid-soluble collagen and pepsin-soluble collagen from the skin of hybrid sturgeon. Journal of Wuhan University of Technology-Mater Science Edition, 34(2019), 950–959. https://doi.org/10.1007/s11595-019-2143-6
45. Wu, J., Kong, L., Zhang, J., & Chen,W. (2019). Extraction and properties of acid-soluble collagen and pepsin-soluble collagen from silver carp (Hypophthalmichthys molitrix) scales: Prerequisite information for fishery processing waste reuse. Polish Journal Environmental Studies, 28(4), 2923–2930. https://doi.org/10.15244/pjoes/93742